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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(3): 19-26

The condition of lipid peroxidation in mice and the effect of fullerene C60 during immune response

Vesnina LE, Mamontova TV, Mykytiuk MV, Kutsenko LO, Bobrova NO, Kutsenko NL, Kaĭdashev IP.

    Research Institute for Genetic and Immunological Grounds of Pathology and Pharmacogenetics, Ukrainian Medical Stomatological Academy, Poltava, Ukraine
DOI: https://doi.org/10.15407/fz58.03.019


Abstract

The aim of this study was to assess the infuence of fullerene C60 on lipid peroxidation (POL) and antioxidant protection during the induction of the immune response to heteroantigen. Balb/c mice were immunized intraperitoneal (i.p.) with sheep erythrocytes for the primary immunization. Water dispersion of fullerene C60 was injected i.p. once at the dose 50 ng to mice on frst, third and sixth days after immunization. During immune response, the increment of malonic dialdehide (MDA) was enhanced in liver, kidneys and heart tissues. Fullerene C60 induced POL during the latent phase of immune response, but inhibited this process during progression of immune response. Activities of superoxide dismutase (SOD) and catalase in liver and spleen tissues were induced after injection of fullerene C60 to intact mice. After immunization, high level of activity of antioxidant enzymes and low level of organs mass factor were determined. Injection of fullerene C60 reduced the activities of SOD and catalase in spleen tissues. The results of our study indicate that fullerene C60 can display positive effect on POL processes and antioxidant enzymes activity which is prob­ably due to membrane’s stabilization action or the ability of fullerene C60 to bind free radicals independently.

Keywords: fullerene C60, lipid peroxidation, antioxidant protection states, immune response to heteroantigen.

Full text: (PDF, in Ukrainian)

References

  1. Berkalo L.V., Bobovich O.V., Bobrova N.A., Geiko O.O., Kaydashev I.P., Kutsenko L.O., Nozhinova O.A., Ryabenko V.V., Sokolenko V. V., Shinkevich V.I. Methods of clinical and experimental research in medicine . Ed. I.P. Kaidasheva. Poltava: Polimet, 2003 . 320 p.
    2.  
  2. Kokhan S.T., Krivosheva E.M. An experimental study of the antioxidant properties of plant adaptogens . Tomsk State University Journal. pharmacy. 2010. No. 4 (50). P. 29-33.
    3.  
  3. Lankin V.Z. Tikhadze A.K., Belenkov Yu.N. Free radical processes are normal and in pathological conditions. M.: Medicine, 2001 .-- 78 p.
    4.  
  4. Makhanova R.S. On the study of lipid peroxidation . Izv. Orenburg state agrarian. un-that. 2011. No. 1 (29), part 2. P. 231-234.
    5.  
  5. Pasteur E.U., Gadfly V.V., Pozur V.K., Vikhot N.E. Immunology: Workshop. K .: Vishka school, 1989 .- 326 p.
    6.  
  6. Piotrovsky L.B. Fullerenes in the design of medicinal substances . Ros. nanotechnology. 2007. 2, No. 7-8. P. 6-18.
    7.  
  7. Roy A., Brostoff J., Mail D. Immunology. M.: Mir, 2000 .- 592 p.
    8.  
  8. Dhavan A., Taurozzi J.S., Pandey A.K. Shan W., Miller S.M, Hasham S.A., Tarabara V.V. Stable colloidal disper­sion of C60 fullerenes in water: evidence for genotoxicity . Environ. Sci. Technol. 2006. 40. P. 7394-7401. CrossRef PubMed
    9.  
  9. Dugan L.L., Gabrielsen J.K., Yu S.P., Lin T.S., Choi D.W. Buckminsterfullerenol free radical scavengers reduce ex-citotoxic and apoptotic death of cultured cortical neurons . Neurol. Dis. 1996. 3(2). P. 129-135. CrossRef PubMed
    10.  
  10. Gharbi N., Pressac M., Hadchouel M., Moussa F. C60 Fullerene Prevents Acute Liver Injury Induced by Carbon Tetrachloride in Rats. [Electronic resource] . F. The Electrochemical Society Interface (205-th Meeting, May 9-14, 2004, San Antonio, TX, USA), Spring 2004, #581 http:.www.electrochem.org. meetings.
    11.  
  11. Krustic P.J., Wasserman E., Kaiser P.N. Radical reaction of C60 .Science. 1991. 254. P. 1183-1185. CrossRef PubMed
    12.  
  12. Meydani S.N., Wu D., Santos M.S., Hayek M.G. Antioxidants and immune response in aged persons: overview of present evidence . Amer. J. Clin. Nutr. 1995. 62. P. 1462-1476. CrossRef PubMed
    13.  
  13. Moussa F., Pressac M., Genin E., Roux S., Trivin F., Rassat A., Ceolin R., Szwarc H.H. Quantitative analysis of C-60 fullerene in blood and tissues by high-performance liquid chromatography with photodiode-array and mass spectro-metric detection . J. Chromatog. B-Analyt. Technolog. in the Biomed. and Life Sci. 1997. 696(1). P. 153-159. CrossRef  
  14. Ryan J.J., Bateman H.R., Stover A., Gomez G., Norton S. K., Zhao W., Schwartz L.B., Lenk R., Kepley C.L. Fullerene nanomaterials inhibit the allergic response . J. Immunol. 2007. 179. P. 665-672. CrossRef PubMed
    15.  
  15. Ros T. Twenty Years of Promises: Fullerene in Medicinal Chemistry . Med. Chem. and Pharmacol. Potential Fulleren. and Carbon Nanotubes. 2008. 1. P. 1-21. CrossRef  
  16. Sameh S.Ali, Hardt J. I., Quick K. L., Kim-Han J. S., Erlanger B. F., Huang T., Epstein C. J., Dugan L. L. A viologicalli effective fullerene (C60) derivative with su-peroxide dismutase mimetic properties . Free Rad. Biol. and Med. 2004. 37(8). R. 1191-1202. CrossRef PubMed
    17.  
  17. Torres V.M., Posa M., Srdjenovic B., Simplicio A.L. Solubilization of fullerene C60 in micellar solutions of different solubilizers .Colloids and Surfaces B: Bioin-terfaces. 2011. 82(1). P. 46-53. CrossRef PubMed
    18.  
  18. Wang I.C., Tai L.A., Lee D.D., Kanakamma P.P., Shen C.K.-F., Luh T.-Y., Cheng C.H., Hwang K.C. C60 and water-soluble fullerene derivatives as antioxidants against radical-initiated lipid peroxidation . J. Med. Chem. 1999. 42, Is. 22. P. 4614-4620. CrossRef PubMed
    19.  
  19. Scrivens W.A., Tour J.M., Creek K.E., Pirisi L. Synthesis of 14C-labeled C60, its suspension in water, and its uptake by human keratinocytes . J. Amer. Chem. Soc. 1994. 116. P. 4517-4518. CrossRef  

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