Effect of tilorone and its analogues on the change of mitochondrial potential of rat hepatocytes
Zholobak NM, Kavok NS, Bogorad-Kobelska OS, Borovoy IA, Malyukina MY, Spivak MY.
- D.K. Zabolotny Institute of Microbiology and Virology of theNational Academy of Sciences of Ukraine, Kyiv, Ukraine;
- Institute for Scintillation Materials of the National Academy of Sciencesof Ukraine, Kharkov, Ukraine
DOI: https://doi.org/10.15407/fz58.02.039
![](/images/cite.png)
Abstract
The influence of tilorone dihydrochloryde and its analogues – diphenyl derivatives on the changes of transmembrane potential of mitochondrial membranes of the isolated rat hepatocytes has been estimated. Authors have shown a significant increase in mitochondrial potential thirty minutes after the introduction of the test compounds to the cells using the fluorescent probe JC-1. These results indicate the rapid activation with tilorone and its analog – dihydrochloryde 4,4'-bis-[2-(diethylamino)ethoxy]diphenyl – of the RLR signaling pathway. The final stage of this pathway is the cell production of IFN type I. The authors concluded that there is an increasing of the organelles resistance to the extra/intracellular damaging agents under the influence of the test compounds.
Keywords:
тилорон, производные дифенила,митохондриальный потенциал, флуоресцентные зонды,гепатоциты крыс.
References
- Averchenko E.A., Kavok N.S., Stepanenko A.M., Borovoy I.A., Malyukina M.Yu. Assessment of mitochondrial potential of isolated hepatocytes when the oxidative status changes . Biophys. Herald. 2009. 22, N 1. P. 49-56.
- Bogatsky O.V., Gren A.I., Litvinova L.O., Lempart G.V. Synthesis of 2,7-bis-[2-(diethylamino) ethoxy] fluoren-9-on . Extras. AS URSR. Ser. B. 1976. N 7. P. 610-612.
- Bogorad-Kobelska O.S., Zholobak N.M., Dolga O.V., Maltzev G.V., Lyakhov S.A., Andronati S.A., Spivak M.Ya. Diphenyl derivatives: cytotoxicity, antiviral and IFN-inducing activities in vitro . Int. J. Biomed. 2011. 1,N 3. P. 153-157.
- Vladimirov Yu.A. Physical and chemical basics of cell pathology. Lecture 3. Impairment of the mitochondria functions in tissue hypoxia. M.: MSU, RSMU. 1998. P. 1-6.
- Kavok N.S., Borovoy I.A., Malyukina M.Yu. Effect of different DMSO concentrations on dynamics of hormone-stimulated changes in transmembrane potentials of isolated rat hepatocytes during assessment with fluorescent probes . Probl. Cryobiol. 2010. 20, N 4. P. 407-415.
- Kiprianov A. I., Pilyugin G. T. Synthesis of polyme-thine dyes . Scient. Notes of Kharkiv Univ. 1937. 10. P. 91-107.
- Lyakhov S.A., Litvinova L.A., Andronati S.A., Berezina L.K., Galkin B.N., Osetrov V.E., Philippova T.O., Golovenko N.Ya. Biochemical mechanisms of realization of the antiviral and interferon-inducing activity of amixine and its analogues . Ukr. Biohim. J. 2001. 73, N 4. P. 108-113.
- Petrenko A.Yu., Grishuk V.P., Sukach A.N., Roslya-kov A.D., Belous A.M. The energy state of rat hepatocytes isolated by use of EDTA and vibration . Biochem. 1989. 54, N (12). P. 1952-1955.
- Shay D.S., Zholobak N.M., Lyahov S.A., Maltzev G.V., Fernandes de Rivas S.O., Litvinova L.O., Andronati S.A., Spivak M.Ya. Interferon activity of amixin analogs and diphenyl derivatives . Microbiol. Zh. 2007. 69, N 5. P. 59-64.
- Koshiba T., Yasukawa K., Yanagi Y, Kawabata S. Mitochondrial membrane potential is required for MAVS-mediated antiviral signaling . Sci. Signal. 2011. 4, N 158. P. ra7.
CrossRef
PubMed
- Mancini M., Anderson B.O., Caldwell E., Sedghinasab M., Paty P.B., Hockenbery D.M. Mitochondrial proliferation and paradoxical membrane depolarization during terminal differentiation and apoptosis in a human colon carcinoma cell line . J. Cell Biol. 1997. 138, N 2. P. 449-469.
CrossRef
PubMed PubMedCentral
- Mancini M., Sedghinasab M., Knowlton K., Tam A., Hockenbery D., Anderson B.O. Flow cytometric measurement of mitochondrial mass and function: a novel method for assessing chemoresistance . Ann. Surg. Oncol. 1998. 5, N 3. P. 287-295.
CrossRef
PubMed
- Mayer G.D., Krueger R.F. Tilorone hydrochloride: mode of action . Science. 169. P. 1214-1215.
CrossRef
PubMed
- Mitochondria in pathogenesis . Eds. by John J. Lemasters and Anna-Liisa Nieminen. New York: Kluwer Acad.. Plenum Publish. 2001. 540 p.
- Reers M., Smith T.W., Chen L.B. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential . Biochem. 1991. 30, N 18. P. 4480-4486.
CrossRef
PubMed
- Seth R.B., Sun L., Chen Z.J. Antiviral innate immunity pathways . Cell Res. 2006. 16, N 2. P. 141-147.
CrossRef
PubMed
- Smiley S.T., Reers M., Mottola-Hartshorn C, Lin M., Chen A., Smith T.W., Steele G.D. Jr, Chen L.B. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1 . Proc. Natl. Acad. Sci. USA. 1991. 88, N 9. P. 3671-3675.
CrossRef
PubMed PubMedCentral
- Stringfellow D.A., Glasgow L.A. Tilorone hydrochloride: an oral interferon-inducing agent . Antimicrob. Agents Chemother. 1972. 2, N 2. P. 73-78.
CrossRef
PubMed PubMedCentral
- Yasukawa K., Oshiumi H., Takeda M., Ishihara N., Yanagi Y, Seya T., Kawabata S., Koshiba T. Mitofusin 2 inhibits mitochondrial antiviral signaling . Sci. Signal. 2009. 2, N 84. P. ra47.
CrossRef
PubMed
|