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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(2): 33-38


Pioglitazone, an activator of PPAR-g, reduces theexpression of kB nuclear factor and inhibits apoptosis in mononuclear cells of peripheralblood in vitro

N.L. Kutsenko, L.E. Vesnina, I.P. Kaidashev

    Research Institute for Genetics and Immunological Groundsof Pathology and Pharmacogenetics, Higher State Educa-tional Establishment of Ukraine “UMSA”, Poltava.


Abstract

In order to examine the impact of PPAR-Y activator on the expression of kB nuclear transcription factor and apoptosis, the peripheral blood mononuclear cells (PBMC) were incu­bated with pioglitazone at concentrations 10, 30 and 100 мM. Expression of NF-kB in CD64+ cells was evaluated by flow cytometry using monoclonal antibodies. The development of apoptosis was evaluated using annexin V and propidium io­dide. Activation of PPAR-Yresulted in a reduction of the number of CD64+ cells and expression of the maximum levels of these receptors. We also obesrved a dose-dependent decrease of NF-kB concentration in PBMC suspension and in CD64+ cells. While analyzing the apoptosis, the reduction of apoptotic cells (reduction of AnV+PI+ cells) has been observed. The re­sults obtained allow to prove that PPAR-Y activation partici­pates in the PBMC apoptosis regulation and in the expression of transcriptional factor NF-kB. Our results indicate for the involvement of PPAR-y in the regulation of NF-kB activity which plays one of the most significant roles in the inflamma­tion realization, oncogenesis, and so on.

Keywords: Peroxisome proliferator-activated receptors-y,pioglitazone, NF-kB nuclear transcription factor, СD64+ cells,apoptosis.

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