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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(2): 27-32


Expression of TNF-a mRNA, butnot of TNF-a receptors mRNA, is detected in single murine oocyte and decreases duringoocyte meiotic maturation: single-cell RT-PCR data

O.A. Shepel, V.E. Dosenko, T.Yu. Voznesenska, R.I. Yanchiy

  1. Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine;


Abstract

Background: The present study was conducted to investigate the expression of TNF-a and its receptors (types I and II) in both oocytes with germinal vesicle and the first polar body in mice. Methods: Oocytes with intact germinal vesicle were isolated from mouse ovaries and subjected to in vitro maturation to obtain oocytes forming the first polar body. A reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of TNF-a and its receptors at mRNA level. Results: mRNA TNF-a was expressed in single oocytes and its level was decreasing during transition from germinal vesicle to the first polar body stage. At the same time the expression of TNF-receptors was not observed in single oocyte. Conclusions: These data are the important link in understanding of the molecular mechanisms regulating oocyte maturation as well as follicle development.

Keywords: фактор некроза опухолей a, рецепторыфактор некроза опухолей a, ооциты, мейотическоесозревание ооцитов.

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