Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(2): 27-32

Expression of TNF-alpha mRNA, but not of TNF-alpha receptors mRNA, is detected in single murine oocyte and decreases during oocyte meiotic maturation: single-cell RT-PCR data

Shepel OA, Dosenko VE, Voznesenska TY, Yanchiy RI.

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


Background: The present study was conducted to investigate the expression of TNF-a and its receptors (types I and II) in both oocytes with germinal vesicle and the first polar body in mice. Methods: Oocytes with intact germinal vesicle were isolated from mouse ovaries and subjected to in vitro maturation to obtain oocytes forming the first polar body. A reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of TNF-a and its receptors at mRNA level. Results: mRNA TNF-a was expressed in single oocytes and its level was decreasing during transition from germinal vesicle to the first polar body stage. At the same time the expression of TNF-receptors was not observed in single oocyte. Conclusions: These data are the important link in understanding of the molecular mechanisms regulating oocyte maturation as well as follicle development.

Keywords: фактор некроза опухолей a, рецепторыфактор некроза опухолей a, ооциты, мейотическоесозревание ооцитов.


  1. Aoki F., Worrad D.M., Schultz R.M. Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo . Dev. Biol. 1997. 181. P. 296-307. CrossRef PubMed
  3. Davidson E.H., Hough B.R. High sequence diversity in the RNS synthesized at the lampbrush stage of oogenesis . Proc. Natl. Acad. Sci. USA. 1969. N 63. P. 342-349. CrossRef PubMed PubMedCentral
  5. Escobar M.L., Echeverria O.M., Sanchez-Sanchez L., Mendez C, Pedernera E., Vazquez-Nin GH. Analysis of different cell death processes of prepubertal rat oocytes in vitro . Apoptosis. 2010. 15, N 4. P. 511-526. CrossRef PubMed
  7. Fiorenza M.T., Mangia F. Quantitative RT-PCR amplification of RNA in single mouse oocytes and pre­implantation embryos . Biotechniques. 1998. 24, N 4. P. 618-623. CrossRef PubMed
  9. Goncalves J.S., Vannucchi C.I., Braga F.C., Paula-Lopes F.F., Milazzotto M.P., Assumpcao M.E., Visintin J.A. Oestrogen and progesterone receptor gene expression in canine oocytes and cumulus cells through­out the oestrous cycle . Reprod. Domest. Anim. 2009. 44 (Suppl 2). P. 239-242. CrossRef PubMed
  11. Greenfeld C.R., Roby K.F., Pepling M.E., Babus J.K., Terranova P.F., Flaws J.A. Tumor necrosis factor (TNF) receptor type 2 is an important mediator of TNF alpha function in the mouse ovary . Biol. Reprod. 2007. 76, N 2. P. 224-231. CrossRef PubMed
  13. Jones G.M., Song B., Cram D.S., Trounson A.O. Op­timization of a microarray based approach for deriving representative gene expression profiles from human oocytes . Mol. Reprod. Dev. 2007. 74, N 1. P. 8-17. CrossRef PubMed
  15. Li S.S., Liu Y.H., Tseng C.N., Singh S. Analysis of gene expression in single human oocytes and preimplanta­tion embryos . Biochem. Biophys. Res. Commun. 2006. 340, N 1. P. 48-53. CrossRef PubMed
  17. Livingston T., Rich K., MacKenzie S., Godkin J.D. Glutathione content and antioxidant enzyme expres­sion of in vivo matured sheep oocytes . Anim. Reprod. Sci. 2009. 116, N 3-4. P. 265-273. CrossRef PubMed
  19. Malcuit C, Trask M.C., Santiago L., Beaudoin E., Tremblay K.D., Mager J. Identification of novel oo­cyte and granulosa cell markers . Gene Expr. Patterns 2009. 9, N 6. P. 404-410. CrossRef PubMed
  21. Marcinkiewicz J.L., Balchak S.K., Morrison L.J. The involvement of tumor necrosis factor-alpha (TNF) as an intraovarian regulator of oocyte apoptosis in the neonatal rat . Front. Biosci. 2002. N 7. P. d1997-2005. CrossRef  
  22. Marcinkiewicz J.L., Krishna A., Cheung C.M., Terranova P.F. Oocytic tumor necrosis factor alpha: localization in the neonatal ovary and throughout fol­licular development in the adult rat . Biol. Reprod. 1994. 50, N 6. P. 1251-1260. CrossRef PubMed
  24. Monti M., Redi C. Oogenesis specific genes (Nobox, Oct4, Bmp15, Gdf9, Oogenesin1 and Oogenesin2) are differentially expressed during natural and gonadotro-pin-induced mouse follicular development . Mol. Reprod. Dev. 2009. 76, N 10. P. 994-1003. CrossRef PubMed
  26. Naz R.K., Zhu X., Menge A.C. Expression of tumor necrosis factor-alpha and its receptors type I and type II in human oocytes . Mol. Reprod. Dev. 1997. 47, N 2. P. 127-133. CrossRef  
  27. Pandey A., Gupta S.C., Gupta N. Effect of FSH and LH hormones on oocyte maturation of buffalo and gene expression analysis of their receptors and Cx43 in maturing oocytes . Zygote. 2010. N 4. P. 1-4. CrossRef PubMed
  29. Rappolee D.A., Brenner C.A., Schultz R., Werb Z. Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos . Science. 1988. 241. P. 1823-1825. CrossRef PubMed
  31. Shepel O.A., Yanchiy R.I. Reactivating action of a small dose of antiovarian antibodies on oocyte meiotic maturation of mice in conditions of oppressing influence of interleukin-2 and tumor necrosis factor-a . Top. Probl. Pharmaceut. and Med. Sci. and Pract. 2007. N 2. P. 245-251.
  33. Shimada M., Ito J., Yamashita Y., Okazaki T., Isobe N. Phosphatidylinositol 3-kinase in cumulus cells is responsible for both suppression of spontaneous matu­ration and induction of gonadotropin-stimulated matu­ration of porcine oocytes . J. Endocrinol. 2003. 179, N 1. P. 25-34. CrossRef PubMed
  35. Spaczynski R.Z., Arici A., Duleba A.J. Tumor necro­sis factor-alpha stimulates proliferation of rat ovarian theca-interstitial cells . Biol. Reprod. 1999. 61, N 4. P. 993-998. CrossRef PubMed
  37. Taft R.A., Denegre J.M., Pendola F.L., Eppig J.J. Iden­tification of genes encoding mouse oocyte secretory and transmembrane proteins by a signal sequence trap . Biol. Reprod. 2002. 67, N 3. P. 953-960. CrossRef PubMed
  39. Terranova P.F. Potential roles of tumor necrosis fac-tor-alpha in follicular development, ovulation, and the life span of the corpus luteum . Domest. Anim. Endocrinol. 1997. 14, N 1. P. 1-15. CrossRef  
  40. Thomas P.S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose . Proc. Natl. Acad. Sci. USA. 1980. N 77. P. 5201-5205. CrossRef PubMed PubMedCentral
  42. White B.A., Bancroft F.C. Cytoplasmic dot hybridiza­tion . J. Biol. Chem. 1982. 257. P. 8569-8572.
  44. Worrad D.M., Ram P.T., Schultz R.M. Regulation of gene expression in the mouse oocyte and early preim­plantation embryo: developmental changes in Sp1 and TATA box-binding protein, TBP . Development. 1994. 120. P. 2347-2357.
  46. Zeng F., Schultz R.M. Gene expression in mouse oo­cytes and preimplantation embryos: use of suppres­sion subtractive hybridization to identify oocyte- and embryo-specific genes . Biol. Reprod. 2003. 68, N 1. P. 31-39. CrossRef PubMed
  48. Zuccotti M., Merico V., Sacchi L., Bellone M., Brink T.C., Stefanelli M., Redi C.A., Bellazzi R., Adjaye J., Garagna S. Oct-4 regulates the expression of Stella and Foxj2 at the Nanog locus: implications for the devel­opmental competence of mouse oocytes . Hum. Reprod. 2009. 24, N 9. P. 2225-2237. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.