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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(1): 86-92

Effects of diazoxide on the mitochondrial membrane potential and ROS generation in rat uterus cells

Vadziuk OB.

    Palladin Institute of Biochemistry of the NAS of Ukraine, Ukraine


In the present study we demonstrate partial depolarization of the mitochondrial inner membrane from the rat uterus cells upon activation of mitochondrial ATP-sensitive K+-channel (mitoKATP) with diazoxide. The estimated affinity constant of diazoxide to mitoKATP from rat uterus cells is (5.01 ± 1.47)-10-6 M. We also observed an enhanced generation of reactive oxy­gen species after addition of diazoxide. Both effects were ef­fectively eliminated by glybenclamide, blocker of the ATP-sensitive K+ channel. Our results indicate that activation of mitoK in rat uterus cells leads to a partial depolarization of mitochondrial membrane and an increase in ROS concentration. Key words: diazoxide, mitochondrial ATP-sensitive K+-channel, reactive oxygen species, membrane potential, uterus.

Keywords: diazoxide, mitochondrial АТP-sensitive К+-channel, reactive oxygen species, membrane potential, uterus.


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