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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(1): 86-92


Effects of diazoxide on the mitochondrial membrane potential and ROS generation in rat uterus cells

Vadziuk OB.

    Palladin Institute of Biochemistry of the NAS of Ukraine, Ukraine
DOI: https://doi.org/10.15407/fz58.01.086

Abstract

In the present study we demonstrate partial depolarization of the mitochondrial inner membrane from the rat uterus cells upon activation of mitochondrial ATP-sensitive K+-channel (mitoKATP) with diazoxide. The estimated affinity constant of diazoxide to mitoKATP from rat uterus cells is (5.01 ± 1.47)-10-6 M. We also observed an enhanced generation of reactive oxy­gen species after addition of diazoxide. Both effects were ef­fectively eliminated by glybenclamide, blocker of the ATP-sensitive K+ channel. Our results indicate that activation of mitoK in rat uterus cells leads to a partial depolarization of mitochondrial membrane and an increase in ROS concentration. Key words: diazoxide, mitochondrial ATP-sensitive K+-channel, reactive oxygen species, membrane potential, uterus.

Keywords: diazoxide, mitochondrial АТP-sensitive К+-channel, reactive oxygen species, membrane potential, uterus.

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