Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(1): 86-92

Effects of diazoxide on the mitochondrial membrane potential and ROS generation in rat uterus cells

Vadziuk OB.

    Palladin Institute of Biochemistry of the NAS of Ukraine, Ukraine
DOI: https://doi.org/10.15407/fz58.01.086


Abstract

In the present study we demonstrate partial depolarization of the mitochondrial inner membrane from the rat uterus cells upon activation of mitochondrial ATP-sensitive K+-channel (mitoKATP) with diazoxide. The estimated affinity constant of diazoxide to mitoKATP from rat uterus cells is (5.01 ± 1.47)-10-6 M. We also observed an enhanced generation of reactive oxy­gen species after addition of diazoxide. Both effects were ef­fectively eliminated by glybenclamide, blocker of the ATP-sensitive K+ channel. Our results indicate that activation of mitoK in rat uterus cells leads to a partial depolarization of mitochondrial membrane and an increase in ROS concentration. Key words: diazoxide, mitochondrial ATP-sensitive K+-channel, reactive oxygen species, membrane potential, uterus.

Keywords: diazoxide, mitochondrial АТP-sensitive К+-channel, reactive oxygen species, membrane potential, uterus.

Full text: (PDF, in Ukrainian)

References

  1. Akao M., Ohler A., O'Rourke B. Marban E. Mito­chondrial ATP-sensitive Potassium Channels inhibit apoptosis induced by oxidative stress in cardiac cells . Circulat. Res. 2001. 88. P. 1267- 1275. CrossRef PubMed
    2.  
  2. Busija D., Katakam P., Rajapakse N.C., Kis B., Grover G., Domoci F., Bari F. Effects of ATP-sensitive potas­sium channel activators diazoxide and BMS-191095 on membrane potential and reactive oxigen species pro­duction in isolated piglet mitochondria . Brain Res. Bull. 2005. 66. P. 85- 90. CrossRef PubMed
    3.  
  3. Cancherini D., Trabuco L., Rebousac N., Kowaltowski A. ATP-sensitive K+-channels in renal mitochondria . Amer. J. Physiol. Renal. Physiol. 2003. 285. P. F1291-F1296. CrossRef PubMed
    4.  
  4. Chien E.K., Zhang Y.Z., Furuta H., Hara M. Expression of adenosine triphosphate-sensitive potassium channel subunits in female rat reproductive tissues: overlapping distribution of messender ribonucleic acid for weak inwardly rectifying potassium channel subunit 6.1 and sulfonylurea-binding regulatory subunit 2 . Amer. J. Obstet. Gynecol. 1999. 180. P. 1121-1126. CrossRef  
  5. Costa A., Garlid K. Intramitochondrial signalling: in­teractions among mitoKatp, PKCe, ROS, and MPT . Amer. J. Physiol. Heart Circulat. Physiol. 2008. 295. P. H874-H882. CrossRef PubMed PubMedCentral
    6.  
  6. Costa A., Jacob R., Costa C, Andrukhiv K., West I., Garlid K. The mechanism by which the mitochondrial ATP-sensitive K+-channel opening and H2O2 inhibit the mitochondrial permeability transition . J. Biol. Chem. 2006. 281, N 30. P. 20801-20808. CrossRef PubMed
    7.  
  7. Curley M., Cairns M.T., Freil A.M. Expression of mRNA transcripts for ATP-sensitive potassium channels in human myometrium . Mol. Hum. Reprod. 2002. 8, N 10. P. 941- 945. CrossRef PubMed
    8.  
  8. Facundo H.T.F., Paula J.G., Kowaltowski A.J. Mito­chondrial ATP-sensitive K+-channels are redox sensi­tive pathways that control reactive oxygen species production . Free Radical Biol. and Med. 2007. 42. P. 1039-1048. CrossRef PubMed
    9.  
  9. Garlid K., Paucek P., Yarov-Yarovoy V. The mitochon­drial Kat-channel as a receptor for potassium channel openers . J. Biol. Chem. 1996. 271. N 15. P. 8796-8799. CrossRef PubMed
    10.  
  10. Garlid K. Paucek P., Yarov-Yarovoi V. Cardioprotec­tive effect of diazoxide and its interaction with mito­chondrial ATP-sensitive K+-channels . Circul. Res. 1997. 81. P.1072-1082. CrossRef PubMed
    11.  
  11. Hanley P. J., Daut J. Katp-channels and precondition­ing: A re-examination of the role of mitochondrial K channels and an overview of alternative mechanisms . J. Mol. Cell Cardiol. 2005. 39. P. 17- 50. CrossRef PubMed
    12.  
  12. Hassouna A., Matata B. M., Galinanes M. PKCe is upstream and PKCa ia downstream of mitoKATP-channels in the signal transduction pathway of ischemic preconditioning of human myocardium . Amer. J. Physiol. Cell Physiol. 2004. 287. P C1418- C1425. CrossRef PubMed
    13.  
  13. Holmuhamedov E., Wang L., Terzic A. ATP-sensitive K+-channel openers prevent Ca2+ overload in rat cardiac mitochondria . J. Physiol. 1999. 519. P. 347- 360. CrossRef PubMed PubMedCentral
    14.  
  14. Lawrence C.L., Billups B. B., Rodrigo G.C., Standen N.B. The KatP-channel opener diazoxide protects cardiac myocytes during metabolic inhibition without causing mitochondrial depolarization or flavoprotein oxidation . Brit. J. Pharmacol. 2001. 134. P. 535-542. CrossRef PubMed PubMedCentral
    15.  
  15. Lebuffe G., Schumacker P.T., Shao Z.H., Anderson T., Iwase H., Vanden Hoek T.L. ROS and NO trigger early preconditioning: relationship to mitochondrial K channel . Amer. J. Physiol. Heart Circulat. PhysioAlT. 2003. 284. P. H299-H308. CrossRef PubMed
    16.  
  16. Mollard P., Mironneau J., Amedee T. Electrophysi­ological characterization of single pregnant rat myometrial cells in short-term primary culture . Amer. J. Physiol. 1986. 19, N 1. P.C. 47- 54. CrossRef PubMed
    17.  
  17. Shien C.C., Coghlan M., Sullivan J. Gopalakrishnan M. Potassium channels: molecular defects, diseases, therapeutic opportunities . Pharmacol. Rev. 2000. 52, N 4. P. 557-593
    18.  
  18. Signal transduction by reactive oxygen and nitrogen spe­cies. Pathways and chemical principles. Eds by Forman H. J., Fucuto J., M. Torres. New York, Boston, Dordrecht, London, Moscow: Kluwer Acad. Publish., 2004. P. 411.
    19.  
  19. Teramoto N. Physiological roles of ATP-sensitive K+-channels in smooth muscle . J. Physiol. 2006. 572, N 3. P. 617- 624. CrossRef PubMed PubMedCentral
    20.  
  20. Warren A.Y, Matharoo-Balls B., Shaw R.W. Hydro­gen peroxide and superoxide anion modulate pregnant human myometrial contractility. Reproduction. 2005. 130. P. 539-544. CrossRef PubMed
    21.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.