Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(1): 56-60

Correction of age-related changes in sphingolipid content in rat tissues by acid sphingomyelinase inhibition

Har'kavenko VV, Storozhenko GB, Krasnikova OM, Babenko NO.

    Institute of Biology, Kharkiv Karazin National University, Ukraine


Because reduction of sphingomyelin and ceramide accumula­tion in aging are associated with the development of a wide variety of pathologies, finding the ways of normalizing the sphingolipid exchange is becoming actual. As the ASM is a key enzyme of the sphingolipid exchange, in the present study we have investigated the effect of an ASM inhibitor imipramine on the ceramide and sphingomyelin content in the tissues of old rats. The introduction of imipramine elevates the sphingo-myelin level and reduces the ceramide/sphingomyelin ratio in the liver, heart and brain of old rats. The hippocampus is most sensitive to the imipramine effects. These data suggest that imipramine is an effective modulator of sphingolipid content in the body of old rats. The age-related changes in the ceramide and sphingomyelin content in the tissues are higly determined by the activation of acid sphingomyelinase in the process of aging. Keywords: sphingomyelin, ceramide, sphingomyelinase, ag­ing, imipramine.

Keywords: sphingomyelin, ceramide, sphingomyelinase, ag-ing, imipramine.


  1. Ipatova O.M., Torkhovskaya T.I., Zakharova T.S. Sphingolipids and cell signaling: participation in apoptosis and atherogenesis . Biochemistry. 2006. 71, no. 7. C. 882-893. CrossRef PubMed
  3. Findlay J.B., Evans W.G. Biological membranes. Methods: Per. from English M .: Mir, 1990 . 424
  5. Babenko N., Shakhova E. Effects of Chamomilla recutita flavonoids on age-related liver sphingolipid turnover in rats . Exp. Gerontol. 2006. 41, N 1. P. 32-39. CrossRef PubMed
  7. Babenko N.A., Semenova Y.A. Effects of long-term fish oil-enriched diet on the sphingolipid metabolism in brain of old rats . Exp. Gerontol. 2010 45, N 5. P. 375-380. CrossRef PubMed
  9. Bartlett G.R. Phosphorus assay in column chromatography . J.Biol. Chem. 1959. 234, N 3. P. 466-468.
  11. Bligh E.G., Dyer W.J. A rapid metod of total lipid extraction and purification . Can. J. Biochem. Physiol. 1959.- 37, N 8.- C. 911-917. CrossRef PubMed
  13. Deupree J.D., Reed A.L., Bylund D.B. Differential effects of the tricyclic antidepressant, desipramine, on the den­sity of adrenergic receptors in juvenile and adult rats . J. Pharmacol. Exp. Ther. 2007. 321, N 2. P. 770-776. CrossRef PubMed
  15. Hannun Y.A., Obeid L.M. The ceramide-centric universe of lipid-mediated cell regulation: stress en­counters of the lipid kind . J. Biol. Chem. 2002. 277, N 29. P. 25847-25850. CrossRef PubMed
  17. Jenkins R.W., Canals D., Idkowiak-Baldys J., Simbari F., Roddy P., Perry D.M., Kitatani K., Luberto C, Hannun Y.A. Regulated secretion of acid sphingo­myelinase implications for selectivity of ceramide formation . Ibid. 2010. 285,N 46. P. 35706-35718. CrossRef PubMed PubMedCentral
  19. Kirschnek S., Paris F., Weller M., Grassme H. , Ferlinz K., Riehle A., Fuks Z., Kolesnick R., Gulbins E. CD95-mediated apoptosis in vivo involves acid sphingo­myelinase . Ibid. 2000. 275, N 35. P. 27316- 7323. CrossRef PubMed
  21. Kornhuber J, Medlin A, Bleich S, Jendrossek V, Henkel AW, Wiltfang J, Gulbins E. High activity of acid sphingomycelinase in major depression . J. Neural. Transm. 2005. 112. P. 1583-1590. CrossRef PubMed
  23. Lowry O.N., Rosebrough N.J., Farr A.L., Randal R.J. Protein measurement with the folin phenol reagent . J. Biol. Chem. 1951. 193. P. 365-375.
  25. March J.B. Weinstein D.B. Simple charring method for determination of lipids . J. Lipid Res. 1966. 7, N 4. P. 574-580.
  27. Osawa Y, Uchinami H., Bielawski J., Schwabe R.F., Hannun Y.A., Brenner D. A. Roles for C16-ceramide and sphingosine 1-phosphate in regulating hepatocyte apoptosis in response to tumor necrosis factor b .J. Biol. Chem. 2005. 280, N 30. P. 27879-27887. CrossRef PubMed
  29. Siskind L.J., Colombini M. The lipids C2- and C16-ceramide form large stable channels. Implications for apoptosis .J. Biol. Chem. 2000. 275, N 49. P. 38640-38644. CrossRef PubMed PubMedCentral
  31. Zhang D. X., Fu-Xian Yi, AI-Ping Zou, Pin-Lan LI Role of ceramide in TNF-6-induced impairment of en-dothelium-dependent vasorelaxation in coronary arteries . Amer. J. Physiol. Heart Circ. Physiol. 2002. 283. P. H1785-H1794. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.