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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(1): 13-26


The changes of metabolism in myocardium at ischemia-reperfusion and activating of the ATP-sensitive potassium channels

Strutyns'kyĭ RB, Kotsiuruba AV, Neshcheret OP, Rovenets' RA, Moĭbenko OO.

    O.O.Bogomoletz Institute of Physiology National Academy of Science of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz58.01.013

Abstract

In experiments on the anaesthetized dogs with modeling of experimental ischemia (90 min) and reperfusion (180 min) it was investigated the changes of biochemical processes in the different areas of heart ( intact, risk and necrotic zone) during intragastric introduction of medicinal form (tablets) of flocalin ( the fluorine-containing opener of ATP-sensitive potassium channels) in a dose 2,2 mg/kg. The data analysis allowed to define a few possible cardioprotective mechanisms of flocalin action at ischemia-reperfusion conditions: the preservation of sufficient levels of de novo (by cNOS) NO synthesis, an inhi­bition of de novo (by iNOS) and salvage (by NADH-depen-dent nitratreductase) NO synthesis, an inhibition of L-arginine degradation by arginase, an inhibition of oxidizing metabolism due to limitation of ROS and RNS generation, inhibition of free arachidonic acid and eicosanoids synthesis, inhibition of AT P and GTP degradations and, possibly, stimulation of pro­tective haem degradation. These changes may prevent forma­tion of toxic peroxynitrite and suggest the possibility of par­ticipating in flocalin–mediated cardioprotective effects of warn­ing a mitochondrial permeability transition pore (MPTP) open­ing and inhibition of apoptosis and/or necrosis of cardiomyo-cytes induced by it.

Keywords: аденозинтрифосфатчувствительныекалиевые каналы, флокалин, ишемия–реперфузия,оксидативный стресс, нитрозативный стресс, перекисноеокисление липидов, de novo синтез NO, реутилизационныйсинтез NO.

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