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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(1): 3-12

Cytochrome c as an amplifier of ROS release in mitochondria

O.V. Akopova, L.I. Kolchinskaya, Nosar V.I., Bouryi V.A., Mankovska I.N., V.F. Sagach

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


The influence of exogenous cytochrome c on reactive oxygen species (ROS) formation and its depen­dence on mitochondrial permeability transition pore (MPTP) opening is studied in rat liver mito­chondria. Fluorescent probe dichlorofluorescein (DCF) was used. It was shown that MPTP activa­tion by increasing concentrations of Ca2+ in the medium results in the increase in mitochondrial ROS production and oxygen consumption, but the decrease in matrix calcium retention, dependent on the amount of added Ca2+. Cytochrome c in the incubation medium does not much influence ROS formation when MPTP opening is blocked by cyclosporine A. However, in the presence of cyto-chrome c MPTP opening is accompanied by dramatic increase in ROS production. Steep rise in DCF fluorescence because of matrix ROS formation is sensitive to MPTP opening and is not resulted from the direct interaction between the probe and cytochrome c outside the mitochondria. To explain obtained data the hypothesis is put forward that MPTP could serve for ROS exchange between the matrix and the medium where heme iron of cytochrome c would act as a catalytic center to enhance ROS production. We suppose that apart of its conventional function, cytochrome c which is not involved in electron transport, could serve in such way as the amplifier of ROS production which in turn would provide a background for the development of apoptosis due to MPTP opening. Key words: mitochondria, Ca2+, mitochondrial permeability transition pore, ROS, cytochrome c.

Keywords: мітохондрії, Ca2+, мітохондріальна пора,активні форми кисню, цитохром c.


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