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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(6): 38-45

Genipin--uncoupling protein inhibitor--reduces the protective effect of ischemic preconditioning

Hoshovs'ka IuV, Shymans'ka TV, Sahach VF

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


The functional significance of uncoupling proteins UCP2 and UCP3 in the cell and the organism remains unknown. There have been reports about their involvement in cellular protec­tion mechanisms underlying the phenomenon of ischemic pre­conditioning. The purpose of this study was to elucidate the role of uncoupling proteins UCP2 and UCP3 in the formation of cardioprotective effect of ischemic preconditioning. In ex­periments on isolated rat hearts we show here an increase in the level of UCP2 and UCP3 gene expression in the heart tissue under the influence of ischemic preconditioning with three episodes of 5 min stopping the flow perfusion. Similar effects were induced by a prolonged ischemia-reperfusion of myocardium. The blockade of the UCP2 activity by genipin (Wako Inc, USA, 10-5 mol/L, isolated heart perfusion for 15 minutes) abolished the protective effect of adaptation to is­chemia. It was concluded that uncoupling proteins take part in the cardioprotective effect of ischemic preconditioning.

Keywords: изолированное сердце, разобщающиебелки UCP2 и UCP3, генипин, ишемическое преконди-ционирование.


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