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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(6): 15-22

Urothelium-dependent modulation of urinary bladder smooth muscle contractions by menthol

Paduraru OM, Filippov IB, Boldyriev OI, Vladymyrova IA, Naĭd'onov VH, Shuba IaM

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


TRPM8 cold receptor/channel is considered amongst the vari­ety of receptors that support and modulate sensory function of urothelium, although the information regarding this is still quite contradictory. Here we have studied the effects of non­specific TRPM8 activator menthol on the contractions of the smooth muscle strips of the rat bladder with intact and removed urothelium, and assessed the expression in them of TRPM8 mRNA using semi-quantitative RT-PCR. Menthol (100 uМ) decreased the basal tone and the amplitude of spontaneous contractions only in the strips with intact urothelium.Irrespective of the presence of urothelium it similarly inhib­ited (by ~45 %) the contractions evoked by high-potassium depolarization. Contractions induced by muscarinic agonist carbachol (1 uМ) were inhibited by menthol much stronger (by ~63 %) if the urothelium was present than without it (by ~12 %). Expression of TRPM8 mRNA in urothelium was not detected, whilst in detrusor smooth muscle it was found very low. We conclude that modulation of contractile responses by menthol is most likely explained by its blocking action on voltage-gated calcium channels of detrusor smooth muscle cells (SMC) and by menthol-stimulated release from urothelium of some factor(s) with relaxant effects on SMCs. Stimulation of the secretion of these factors from urothelial cells most likely involves menthol-induced, TRPM8-independent mobilization of calcium.

Keywords: bladder, urothelium, detrusor, smooth muscles,menthol, cold receptor TRPM8.


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