Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(4): 77-81


Effect of vasopressin on the membrane potentialof rat hepatocytes

P.K. Tsapenko, T.P. Liashchenko.

    Київ. нац. ун-т імені Тараса Шевченка


Abstract

The current-voltage relationship of rat hepatocytes was investigated and its linear dependence was shown. On liver slices, we have investigated the effects of vasopressin on membrane potential of rat hepatocytes using microelectrode technique. Vasopressin evoked membrane hyperpolarization to -50 m V, suggesting that vasopressin activated synthetic processes in hepatocytes. Vasopressin application together with the inhibitor of protein kinase C chelerytrine also caused hyperpolarization of hepatocytes, but membrane potential changes were of smaller amplitude. This fact suggests that although protein kinase C plays an important role in the realization of effects of vasopressin, there is, however, another signal transduction pathway of vasopressin in rat hepato-cytes. If that pathway activates adenylatecyclase, vasopressin can stimulate bile formation in hepatocytes. This assumption should be verified.

Keywords: vasopressin, hepatocytes, membrane potential,protein kinase C.

References

  1. Фролькис В.В. Влияние активации биосинтеза белка на уровень мембранного потенциала клеток // Биофизика. - 1974. - 19. - №3. - С.470-473.
  2. Aromataris E.D., Roberts M.L., Barritt G.J., Rychkov G.Y. Glucagon activates Ca2+ and Cl- channels in rat hepatocytes // J. Physiol. - 2006. - 573. - Issue 3. -P.611-625.
  3. Boyer J.L., Nathanson M.H. Bile formation // Shif. Des. liver. - Philadelphia. - 1999. - P.1 19-142.
  4. Cohen A.J., Burczynski F.J., Rosser B.G., Lipschitz J., Minuk G.Y. The effects of various organ preserva­tion solutions on hepatocyte membrane potentials, intracellular calcium concentrations, and outcome fol­lowing liver transplantation // Amer. J.Surg. - 2000. -179(2). - P.154-160.
  5. Dupont G., Tordjmann T., Clair C, Swillens S., Claret M., Combettes L. Propagation mechanisms of receptor-oriented intercellular calcium waves in hepa­tocytes // FASEB J. - 2000. - 14. - P.279-289.
  6. Ferguson J.W., Therapondos G., Newby D.E., Hayes P.C. Therapeutic role of vasopressin receptor antago­nism in patients with liver cirrhosis // Clin. Sci. - 2003. -105. - P.1-8.
  7. Field A.C., Jenkinson J.H. The effect of noradrenaline on the ion permeability of isolated mammalian hepato­cytes, studied by intracellular recording // J. Physiol. -1987. - 392. - P.493-512.
  8. Kingler C, Ancellin N., Barrault M.B., Morel A., Corman B. Potentiation of receptor-mediated cAMP production: role in the cross-talk between vasopressin V1a and V2 receptor transduction pathways // Biochem.J. - 1998. - 330. - P.1023-1028.
  9. 9. Kuhn W.F., Gewirtz D.A. Stimulation of taurocholate and glycocholate efflux from the rat hepatocyte by arginine vasopressin // Amer. J.Physiol. Gastrointest. Liver Physiol. - 1990. - 254, № 17. - P.732-740.
  10. 10. Li A.P. Human hepatocytes: isolation, cryopreserva-tion and applications in drug development // Chem.-Biol. Interact. - 2007. - 168(1). - P.16-29.
  11. Lyall V., Croxton T.L., Armstrong W.M. Measurment of intracellular chloride activity in mouse liver slices with microelectrodes // Biochim. and Biophys. Acta. -1987. - 903(1). - P.56-67.
  12. Nicou A., Serrier V., Prigent S., Boucherie S., Combettes L., Gillon G., Allonso G., Tordjmann Th. Hypothalamic vasopressin release and hepatocyte Ca2+ signaling during liver regeneration: an interplay stimulating liver growth and bile flow // FASEB J. - 2003. - 17. - P.1901-1903.
  13. Poisner R.H. The secretory process. Vol.2. The elec-trophysiology of the secretory cell. - Amsterdam: Elsevier, 1985. - 216 p.
  14. SawanaboriT., Takanashi H., Hiraoka M., Iida Y., Kamisaka K., Maezawa H. Electrophysiological prop­erties of isolated rat liver cells // J. Cell. Physiol. -1989. – 139. -№3. - P.580-585.
  15. Schmeisch A.P., de Oliveira D.S., Ide L.T., Suzuki-Kemmelmeier F., Bracht A. Zonation of the metabolic action of vasopressin in the bivascularly perfused rat liver // Regul. Pept. - 2005. - 129. - P.233-243.
  16. Serriere V, Berthon B., Boucherie S. Vasopressin receptor distribution in the liver controls calcium wave propagation and bile flow // FASEB J. - 2001. - 15. -P.1484-1486.
  17. Spruce B.A., McCulloch A.J., Burd J. The effect of vasopressin infusion on glucose metabolism in man // Clin.Endocrinol. - 1985. - 22. - P.463-468.
  18. Takanashi H., Sawanobori T., Kamisaka K., Maezawa H., Hiraoka M. Properties of single potassium channels in guinea pig hepatocytes // J. Cell Physiol. - 1994. -161(3). -P.537-543.
  19. 19. Tsuda A., Tanaka K.A., Huraux C, Szlam F., Sato N., Yamaguchi K., Levy J.H. The in vitro reversal of hista-mine-induced vasodilation in the human internal mammary artery // Anest. Analg. - 2001. - № 93. -P.1453-1459.
  20. 20. Yacoub D., Theoret J.-F., Villeneuve L., Abou-Saleh H. Mourad W., Allen B.G., Merhi Y Essential Role of proteine kinase C in platelet signaling, IIb3 activation and thromboxane A2 release // J. Biol. Chem. - 2006. -281. - Issue 40. - P.30024-30035.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.