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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(4): 68-76

Change in cryolability of tumor stem cells depending on growth phase of Ehrlich adenocarcinoma in vivo

Hol'tsev AM, Safranchuk OV, Bondarovych MO, Ostankov MV

    Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine


Cancer stem cells (CSC) are the main structural unit in initia­tion, support of in situ growth, as well as tumor metastasis during malignant development of various origin. Observed chemo- and radio-resistance of CSC make them a potential target cell to perform cryoeradication. We have performed a comparative study of changes in functional activity after cryo-preservation of CSC of two differentiation levels from Erlich adenocarcinoma (EAC) of various culture terms (EAC-7, EAC-14). It was found that in the case of EAC-7 the expression of cryosuppression in terms of cell functional state at the initial stages of development was bigger in high potency CD44hi cells comparing to more differentiated cells, forming general pool of cells in peritoneal cavity. And vice versa, in the “eldering” EAC-14 this effect was bigger in more differentiated cells comparing to less differentiated ones, even causing in latter (CD44hi) the growth stimulation. Moreover, the minimal pro-liferative activity of general population cells cEAC-14 during the first weeks was alternated at the next stage to its maximal expression among all the studied types of EAC cells of both culture terms. These data confirm again the fact, that during assessment of bioobject cryolability (cryostability) it is sig­nificant to determine not only cryopreservation conditions, but the initial state of the bioobject as well.

Keywords: cancer stem cells, cryopreservation, Erlich adeno-carcinoma.


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