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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(4): 57-67

Study of changes in the parametres of oxidative stress, anti-oxidative defence and endothelial dysfunction in acute ischemia-reperfusion of the lower extremities in humans

Kakhanovs'kyĭ EF, Sahach VF

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


We carried out a study on elderly people (70 ± 2,2 years old) operated on as an acute occlusion of the femoral artery at its different anatomical levels. In those people, development of oxidative stress has been shown in injured lower extremity in the process of restoring of circulation. Marked increase in catalase activity in the blood gives evidence for work of the anti-oxidative defence system with tense. We determined an increase in aspartataminotransferase, alaninaminotransferase and creatininkinase in ischemia/reperfusion which are widely used to identify injures in muscular tissues. It has been shown that NO2- content in the blood at reperfusion depended on the level of nitrites and the duration of ischemia. Significant changes in NO metabolite content in the blood of the patients suffering from acute occlusion injures of the arteries in the lower ex­tremities could be related with endothelial dysfunction.

Keywords: оксидативный стресс, антиоксидантнаязащита, ишемия–реперфузия, эндотелиальная дисфункция.


  1. Akopova OV, Kotsiuruba AV, Tkachenko IuP, Sahach VF.  Nitric oxide suppresses permeability transition pore opening and enhances calcium uptake in mitochondria in vivo. . Fiziol Zh. 2005. 51, N 3. P. 3-11.
  3. Beridze M.S., Urusadze I.T., Shakarishvili P.P. (2001) Mechanisms of delayed neuronal death in acute cerebral ischemia in an experiment . Journal. neurology and psychiatry. S.S. Korsakova. Stroke (app). 3. P. 35-40.
  5. Gavrilov VB, Mishkorudnaya MI Spectrophotometric determination of lipid hydroperoxide content in blood plasma . Lab. business. 1983. N 3. P. 34-37.
  7. Kamyshnikov B.C. What the medical tests say. Reference manual. Minsk: Belarus. Science, 1997. 197 p.
  9. Kataev SS, Chukhustova B.C., Akimov PA, Obutana LA Spectrophotometric method for determination of nitrites in corpse blood . Current problems of pathological anatomy and forensic medicine: Interuniversity. Sat. scientific works. 2001. P. 158-161.
  11. Kovalenko VN, Gulaya NM, Semikopnaya TV, Kotsiuruba AV, Kornienko TM Disruption of endothelial function in patients with coronary heart disease in combination with hypertension . Ukr. cardiol. . 2002. 3. C. 5-8.
  13. Korobeinikova EN Modification of determination of lipid peroxidation products in reaction with thiobarbituric acid . Lab. business. 1989. N 7. WITH. 8-9.
  15. Koroleuk MA, Ivanova LI, Mayorova IG, Tokarev VE A method for determining the activity of catalase . Lab. business. 1988. N 1. P. 16-19.
  17. Koshkin VM Conservative treatment of chronic arterial insufficiency of the lower extremities . Ed. Savelyeva B.C. M .: Media Medica, 2003, pp. 84-90.
  19. Kulchitsky O.K. Endothelial function and the process of aging . Treatment and diagnosis. 2002. 4. P. 6-9.
  21. Lapshina LA, Kravchuk PG, Titova A.Yu., Glebova OV The importance of determination of nitrite-nitrates as markers of endothelial dysfunction in cardiovascular pathology . Ukr. medical magazine. 2009 6 (74). Pp. 49-52.
  23. Lapshina LA, Titova A.Yu., Gorbach TV, The level of S-nitrosothiol and nitrite-nitrates in serum in chronic heart failure in elderly and elderly patients . Experim. and clinical medicine. 2005. 2. P. 61-64.
  25. Mala LT, Korzh AN, Balkova LB Endothelial dysfunction in pathology of the cardiovascular system. Kharkiv: Torsing, 1999. 432 p.
  27. Malyshev IY, Manukhina EB Stress, adaptation and nitric oxide . Biochemistry. 1998. 63. P. 992-1006.
  29. Manukhina EB, Malyshev I.Yu. The role of nitric oxide in cardiovascular pathology: the view of the pathophysiologist . Ross. cardiol. . 2000. 5. P. 55-63.
  31. Menshchikova EB, Zenkov NK, Reutov VP Nitrogen oxide and NO synthase in mammals under different conditions . Biochemistry. 2000. 65. N 4. p. 485-503.
  33. Motavkin PA, Gelzer BI Clinical and experimental pathophysiology of the lungs. M .: Science, 1998. 336 p.
  35. Palivoda SN, Cherepok AA The role of oxidative stress and impaired nitric oxide metabolism in hypertension . Heart and blood vessels. 2004. 5 (1). C. 39-44.
  37. Utility Model Patent No. 23948 Ukraine. A method for evaluating the development of oxidative stress in the ischemic lower extremity . Kakhanovsky EF, Sagach VF . Bul.N 8, 2007.
  39. Saveliev B.C., Koshkin V.M. Critical lower limb ischemia. M .: Medicine, 1997. 160 p.
  41. Sahach VF, Shymans'ka TV, Nadtochiĭ SM. Factor released during myocardial ischemia reperfusion may become a marker of opening of the mitochondrial permeability transition pore. . Fiziol Zh.- 2003.  49, N 4. p. 6-12.
  43. Sarkisov KG, Korkushko OV, Stupina AS Duzhak GV, Lishnevskaya V.Yu. Microcirculation and hemorreology in human aging . Problems of aging and longevity. 1998. 7, N 3. P. 269-278.
  45. Sokolovich AG, Sokolovich GE, Dambayev G.S. New aspects of pathogenesis, diagnosis and treatment of critical lower extremity ischemia. Tomsk, 2001. 485 p.
  47. Yakovleva LN, Savchenko NP, Stoginchuk OV Antioxidant correction of endothelial dysfunction in patients with ischemic heart disease . Medicine of the world. 2004. Appendix. 2. P. 4.
  49. Bredt D.S., Hwang P.M., Glatt C.E., Lowenstein C.L., Reed R.R., Snyder S.H. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase . Nature. 1991. 351. P.714-718. CrossRef PubMed
  51. Brookes P.S., Darley-Usmar V.M. Role of calcium and superoxide dismutase in sensitizing mitochondria to peroxynitrite-induced permeability transition . Amer. J. Physiol. Heart Circ.Physiol. 2004. 286, N 1. P.H39-H46. CrossRef PubMed
  53. Chartrain N.A., Geller D.A., Koty P.P., Sitrin N.F., Nussler A.K., Hoffman E.P., Billiar T.R., Hutchinson N.I., Mudgett J.S. Molecular cloning, structure, and chromosomal localization of the human inducible ni­tric oxide synthase gene . J. Biol. Chem.. 1994. 269. P. 6765-6772.
  55. Chou T.C., Ven M.H., Li C.V., Ding Y.A. Alterations of nitric oxide synthase expression with aging and hy­pertension in rats . Hypertension. 1998. 31, 2. P.643-648. CrossRef PubMed
  57. Heermeier K., Galle J. Oxidative stress mediated apoptosis induced by oxLDL . Kidney Int. 1999. 56. P. 1310-1312. CrossRef PubMed
  59. Hunter P., Haworth R., Sousard J. Relationship be­tween configuration, function and permeability in calcium treated mitochondria . J. Biol. Chem. 1976. 251. P.5069-5077.
  61. Ji L.L. Antioxidants and oxidative stress in exercise . Proc. Soc. Exp. Biol. Med. 1999. 222. P. 283-292. CrossRef PubMed
  63. John S., Schmieder R.E. Impraired endothelial func­tion in arterial hypertension and hypercholesterolemia:potential mechanisms and differences . J. Hypertens. 2000. 18. P. 363-374. CrossRef PubMed
  65. Kannel W. Prospects for prevention of coronary dis­ease in the elderly. Amer. J. Geriatric Cardiol. 2002. 9, N 2. P. 83-86. CrossRef PubMed
  67. Kim J.S., Ohshima S., Pediaditakis P., Lemasters J.J. Nitric oxide protects rat hepatocytes against reperfusion injury mediated by the mitochondrial per­meability transition . Hepatology. 2004. 39, N 6. P.1533-1543. CrossRef PubMed
  69. Kowaltowski A., Castilho R.F., Vercesi A.E. Mitochon­drial permeability transition and oxidative stress . FEBS Lett. 2001. 495. P.12-15. CrossRef  
  70. Kroemer G. Mitochondrial control of apoptosis: an introduction . Biochem. Biophys. Res. Commun. 2003. 304, N 3. P. 433-435. CrossRef  
  71. Lyons D., Roy S., Patel M. Impaired nitric oxide-me­diated vasodilatation and total body nitric oxide pro­duction in healthy old age . Clin.Sci.(Lond). 1997. 93, N 6. P. 519-526. CrossRef PubMed
  73. Marzinzig M., Nussler A., Sradler J. Improved methods to measure end products of nitric oxide in biological fluids: nitrite, nitrate, and S-nitrosothiols . Nitric. Oxide. 1997. N 1, 2. P.177-189. CrossRef PubMed
  75. Moshage H, Kok B, Huizenga J.R. Nitrite and nitrate determinations in plasma :a critical evaluation . Clin. Chem. 1995. 41, N 6, Pt1. P.892-896.
  77. Rosenfield M.E. Inflammation, lipids and free radicals: Lessons learned from the atherogenic process . Semin. Reprod. Endocrinol. 1999. 16. P. 249-261. CrossRef PubMed
  79. Tanaka S, Yashiro A, Nakashima Y. Plasma nitrite. ni­trate level is inversely connected with plasma low-density lipoprotein cholesterol level. Clin.Cardiol. 1997. 20,N 4. P.361-365. CrossRef PubMed PubMedCentral
  81. Tavaf-Motamen H., Miner T.J., Starnes B.W. Nitric oxide mediates acute injury by modulation of inflam­mation . J. Surg. Res. 1998. 78, N 2. P.137-142. CrossRef PubMed
  83. Tsikas D. Methods og quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological liquids . Free Radic. Res. 2005. 39, N 8. P.797-815. CrossRef PubMed
  85. Vinikka L. Nitric oxide as a challenge of laboratory-clinical chemistry . Scand. J.Clin. Lab.Invwst. 1996. 56, N 7. P. 577-581. CrossRef PubMed

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