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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(4): 46-56


The endothelium . derived hyperpolarizing factor:a reserve defence mechanism of vasodilatation under radiation impact

I.V.ivanova, O.V.kislova, A.I. Soloviev.

    Ін-т фармакології та токсикології АМН України


Abstract

The goal of this study was to determine the cellular mecha­nisms of vascular endothelial dysfunction in rats irradiated with Y-rays. Acetylcholine (Ach)-induced relaxation of rat tho­racic aorta rings was measured as a test of endothelial integrity and function. The data obtained allow suggest that endothelial function is impaired in aorta from g-irradiated rats mainly due to the loss of EDRF/NO-dependent, but not EDHF-dependent relaxation. It has been shown that г-irradiation reduced the Ach-induced NO-release measured as nitrite anion content. Experiments on isolated rat aortic smooth muscle cells using whole-cell patch clamp technique demonstrated that irradiation led to a significant decrease in outward potassium currents. However, Y-ray irradiation was without effect on K+-current carried through apamine-sensitive channels while the current through charybdotoxin-sensitive channels was increased as compared to cells from control animals. The data suggest that EDHF is resistant to ionized radiation and may constitute a crucial reserve mechanism for maintenance of blood flow un­der radiation. Therefore, it is likely that the subsequent stud­ies related to EDHF identification will be important for new drugs development and targeted pharmacological intervention at endothelium dysfunction in case of radiation impact.

Keywords: endothelium-derived hyperpolarizing factor, ni-Ендотелійзалежний фактор гіперполяризаціїISSN 0201-8489 Фізіол. журн., 2011, Т. 57, № 4 55tric oxide, endothelium, potassium channels, smooth musclecells, irradiation

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