Comparative effects of menthol and icilin on the induced contraction of the smooth muscles of the vas deferens of normal and castrated rats
Vladymyrova IA, Filippov IB, Kuliieva IeM, Iurkevych A, Skryma R, Prevarskaia N, Shuba IaM
- O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
- International Center of Molecular Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
- Department of Pharmacology, Escola Paulisata de Medicina, Universiade Federal de Sao Paulo, Brazil
- Laboratoire de Physiologie Cellulaire, Universite des Sciences et Technologies de Lille, France
DOI: https://doi.org/10.15407/fz57.04.021

Abstract
Non-specific TRPM8 agonist menthol was shown to inhibit voltage- and agonist-evoked contractions of the smooth muscle (SM) of rat vas deferens. Here we compared the action of menthol with the action of more specific TRPM8 agonist icilin on depolarization-(60 mM KC1), carbachol-(CCh) and noradrenalin-(Nor)-evoked contractions of the SM strips from the prostatic and epididymal portions of the vas deferens of normal and castrated (60-137 days) rats. Inhibitory action of menthol (100 uM) and icilin (10 uM) on the amplitude of KCl-, CCh- and Nor-induced contractions of normal as well as castrated rats was similar consisting about 50%, despite castration per se strongly potentiated CCh- and Nor-evoked contractions compared to the control animals. In the epididymal portion of the control animals menthol suppressed KCl- and CCh-evoked contractions by 46±5% and 32±3% and icilin by only 14±4% and 6±7%, respectively, whilst after castration both compounds became virtually ineffective. Considering that TRPM8 may localize in the sarcolemma and sarcoplasmic reticulum (SR) membrane and that menthol can also block voltage-gated calcium channels (VGCCs), our data indicate that in the prostatic portion TRPM8 modulates contractility by primarily decreasing the SR Ca2+ stores content, whilst in the epididymal one by both decreasing the SR filling and supporting Ca2+ entry. Drop in the circulation androgens as a result of castration changes the menthol- and icilin-mediated modulation of the rat vas deferens SM contractility via the decrease of the expression of L-type VGCCs and increase of the expression of TRPM8.
Keywords:
vas deferens, гладкие мышцы, TRPM8,ментол, исилин, норадреналин, карбахолин, кастрация.
References
- Boldyrev OI, Sotkis HV, Kuliieva IeM, Vladymyrova IA, Filippov IB, Skryma R, Prevars'ka N, Shuba IaM. Expression of the cold receptor TRPM8 in the smooth muscles of the seminal ejaculatory ducts in rats. . Fiziol Zh. 2009. 55, N 5. P. 17-27.
- Filippov IB, Vladymyrova IA, Kuliieva IeM, Skryma R, Prevarskaia N, Shuba IaM. [Modulation of the smooth muscle contractions of the rat vas deferens by TRPM8 channel agonist menthol]. . Fiziol Zh.. 2009. 55, N 6. P. 30-40.
- Amobi N.I., Smith I.C. Different actions in the rat prostatic and epididimal vas deferens of cyclopiazonic acid or ryanodine on noradrenaline-induced contractions . Gen. Pharmacol. 1999. 32, N 2. P. 271-278.
CrossRef
- Bidaux G., Flourakis M., Thebault S., Zholos A., Bech B., Gkika D., Roudbaraki M., Bonnal Y-J., Mauroy B., Shuba Ya., Skryma R., Prevarskaya N. Prostate cell differentiation status determines transient receptor potential melastatin member 8 channel subcellular localization and function . J. Clin. Invest. 2007. 117, N 6. P. 1647-1657.
CrossRef
PubMed PubMedCentral
- Castillo C.J., Lafayette S., Caricati-Neto A., Sette M., Jurkiewicz N.H., Garcia A.G., Jurkiewicz A. Low digidropyridine receptor density in vas deferentia of castrated rats . Brit. J. Pharmaccol. 1992. 105, N 2. P. 257-258.
CrossRef
PubMed PubMedCentral
- Docherty R.J., Yeats J.C., Piper A.S. Capsazepine block of voltage-activated calcium channels in adult rat dorsal root ganglion neurones in culture . Ibid. 1997. 121, N 7. P. 1461-1467.
CrossRef
PubMed PubMedCentral
- McKemy D.D., Neuhausser W.M., Julius D. Identification of a cold receptor reveals a general role for TRP channels in thermosensation . Nature. 2002. 416, N 6876. P. 52-58.
CrossRef
PubMed
- Miranda H.F., Duran E., Fernandez E., Pinardi G. Muscarinic receptor subtypes in the bisected vas deferens of the rat . Gen. Pharmacol. 1995. 26, N 2. P. 387-391.
CrossRef
- Rohacs T., Nilius B. Regulation of transient potential (TRP) channels by phosphoinositides . Pflug. Arch. 2007. 455, N 1. P. 157-168.
CrossRef
PubMed
- Starling A.P., Hughes G., East J.M., Lee A.G. Mechanism of stimulation of the calcium adenosinet-riphosphatase by jasmone . Biochemistry. 1994 Mar 15; 33(10). P. 3023-3031.
CrossRef
PubMed
- Swandulla D., Carbone E., Schafer K., Lux H.D. Effect of menthol on two types of Ca currents in cultured sensory neurons of vertebrates . Pflug. Arch. 1987. 409. P. 52-59.
CrossRef
PubMed
- Thebault S., Lemonnier L., Bidaux G., Flourahis M., Bavancoffe A., Gordienko D., Roudbaraki M., Delcourt Ph., Panchin Yu., Shuba Ya., Skryma R., Prevarskaya N. Novel role of cold. menthol-sensitive transient receptor potential melastatine family member 8 (TRPM8) in the activation of store-operated channels in LNCaP human prostate cancer epithelial cells . J. Biol. Chem. 2005. 280. P. 39423-39435.
CrossRef
PubMed
- Tsuzuki K., Xing H., Ling J., Gu L.G. Menthol-induced Ca2+ release from presynaptic Ca2+ stores potentiates sensory synaptic transmission . J Neurosci. 2004. 24. P. 762-771.
CrossRef
PubMed PubMedCentral
- Vladimirova I.V., Hirata H., Jurkiewicz N.H., Jurkiewicz A. Interconversion of agonist-induced responses of prostatic and epididymal portion of the rat vas deferens induced by castration . Neurophisiology (Kiev). 2003. 35, N 3. 4. P. 302-310.
CrossRef
- Voets T., Owsianik G., Nilius B. TRPM8 . Handb. Exp. Pharmacol. 2007. 179. P. 329-344.
CrossRef
PubMed
- Zholos A.V. Regulation of TRP-like muscarinic cation current in gastrointestinal smooth muscle with special reference to PLC. Ins3. Ca2+ system . Acta Pharmacol. Sin. 2006. 27, N 7. P. 833-842.
CrossRef
PubMed
|