The effect of hydrogen sulfide on contractile activity of the vascular smooth muscles in rats
Semenykhina OM, Baziliuk OV, Korkach IuP, Sahach VF
Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz57.04.003
Abstract
The effect of endogenous and exogenous hydrogen sulfide (H2S) on contractile activity of vascular smooth muscle (VSM) was studied. The introduction of substrate synthesis H2S L-cys-teine and its donor NaHS in vitro caused concentration-dependent relaxation of VSM of aorta and portal vein. Low concentrations of hydrogen sulfide donor (10-5 mol/L) caused vasoconstriction of both types of the vessels. It was shown that the reaction of relaxation of VSM in response to NaHS is independent from endothelium. It was revealed that VSM of portal vein are more sensitive to the effects of H2S than VSM of aorta. Removing of aorta periadventitial adipose tissue showed no relaxation reply to the hydrogen sulfide donor NaHS in 70% of experiments. Some of the cellular mechanisms of hydrogen sulfide action were established, namely relaxation of aorta is depended on KATP channel activation. This is manifested by a lack of relaxation of the aortic VSM due to KATP channel inhibitor glibenclamide.
Keywords:
сероводород, L-цистеин, NaHS, глибен-кламид, аорта, воротная вена, эндотелий, адвентиция.
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