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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(3): 62-68

Altered collagene properties and lysyl oxidase activity during lathyrism

T.T. Volodina, N.D. Dzvonkevich, L.M. Petrun, I.P. Krisuk, N.M. Popova, S.G. Shandrenko,M.P. Dmytrenko.

    Ін-т біохімії ім. А.В. Палладіна НАН України, Київ


Experiments were carried out on rats with lathyrism, which was induced by adding semicarbazide (0.075%) into drinking water for 45 days. The data obtained show a 30% reduction in the body weight and an increase in.organ weight coefficients. Semicarbazide intake led to the pelvic limb paralysis, scolio-sis, bone tissue degradation, cartilage growth, 46% decrease of the calcium level in the femur. It has been detected essential structural changes in extracellular matrix based on the collagen cross-links reduction. The activity of lysyl oxidase, a key enzyme for the collagen development, showed 5-fold decrease in the aorta tissues. The level of formaldehyde, a nonenzymic cross-links developer, has been measured in the liver tissue by the aldehyde trap (5,5-dimethylcyclohexane-1,3-dione) admin­istration and then fluorimetric determination of formal-dimedone. Under semicarbazide load, the formaldehyde level in the liver tissue was reduced by 47%. Therefore, semicarbazide influences not only the enzymic development of aldehyde groups in collagen, but the level of other alde­hydes, which can cause cross-links. This experimental model of lathyrism is appropriate for investigation of the lysyl oxi-dase inhibitors effect on extracellular matrix.

Keywords: lathyrism, semicarbazide, collagen, lysyl oxidase,cross-links, formaldehyde


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