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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(3): 62-68

Altered collagene characteristics and lysyl oxidase activity in lathyrism

Volodina TT, Dzvonkevych ND, Petrun' LM, Krysiuk IP, Popova NM, Shandrenko SH, Dmytrenko MP

    Palladin’s institute of biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine


Experiments were carried out on rats with lathyrism, which was induced by adding semicarbazide (0.075%) into drinking water for 45 days. The data obtained show a 30% reduction in the body weight and an increase in.organ weight coefficients. Semicarbazide intake led to the pelvic limb paralysis, scolio-sis, bone tissue degradation, cartilage growth, 46% decrease of the calcium level in the femur. It has been detected essential structural changes in extracellular matrix based on the collagen cross-links reduction. The activity of lysyl oxidase, a key enzyme for the collagen development, showed 5-fold decrease in the aorta tissues. The level of formaldehyde, a nonenzymic cross-links developer, has been measured in the liver tissue by the aldehyde trap (5,5-dimethylcyclohexane-1,3-dione) admin­istration and then fluorimetric determination of formal-dimedone. Under semicarbazide load, the formaldehyde level in the liver tissue was reduced by 47%. Therefore, semicarbazide influences not only the enzymic development of aldehyde groups in collagen, but the level of other alde­hydes, which can cause cross-links. This experimental model of lathyrism is appropriate for investigation of the lysyl oxi-dase inhibitors effect on extracellular matrix.

Keywords: lathyrism, semicarbazide, collagen, lysyl oxidase,cross-links, formaldehyde


  1. Volodina TT, Pechenova TM, Dzvonkevich ND, Popova NM, Silonova NV, Astakhova B.C., Panchenko LM, Mikhailovsky VA, Guliy MF Modifying effect of low molecular weight metabolites on the extracellular matrix of animals. . Ukrainian. biochem. . 2007. 79, N 6. P. 52-65.
  3. Dmitrenko MP, Shandrenko SG, Petrun LM, Kishko TO, Silonova NV, Latishko NV, Gudkova OO, Sushkova VV Formaldehyde exchange by semicarboside intoxication . Ukr. biochem. . 2010. 82. P. 39-44.
  5. Zaides AL, Mikhailov AN, Pushchenko OI A modified method for the determination of oxyproline . Biochemistry. 1964. 29, N 1. p. 5-7.
  7. Merkuryeva RV Method of electrophoretic separation of glycosaminoglycans of different types of connective tissue and biological fluids . Vopr. honey. chemistry. 1975. 21 (4). P. 423-428.
  9. Abe M., Takahashi M., Horiuchi K., Nagano A. The changes in crosslink contents in tissues after formalin fixation. Anal. Biochem. 2003. 318(1). P.118-123. CrossRef  
  10. Barrow M.V., Simpson C.F., Miller E.J. Lathyrism: A Review .Quart. Rev. Biol. 1974. 49. P. 101-128. CrossRef PubMed
  12. Bondareva A., Downey Ch. M., Ayres F., Liu W., Boyd S. K., Hallgrimsson B., Jirik F.R. The lysyl oxidase inhibitor, beta-aminopropionitrile, diminishes the meta­static colonization potential of circulating breast cancer Cells.PLoS ONE. 2009. 4(5). 5620. P.1-10. CrossRef PubMed PubMedCentral
  14. El Rouby D.H., Bashir M.H., Korany N.S. Ultrastructural and histomorphometric alterations of rat jaw bones after experimental induction of lathyrism .Arch. Oral Biol. 2008. 53(10). P. 916-923. CrossRef PubMed
  16. Erler J.T., Kevin L., Bennewith K.L., Nicolau M., Dornhofer N., Kong C, Le Q.T. Lysyl oxidase is es­sential for hypoxia-induced metastasis . Nature. 2006. 440. P.1222-1226. CrossRef PubMed
  18. Giampuzzi M., Oleggini R., Di Donato A. Altered adhe­sion features and signal transduction in NRK-49F cells transformed by down-regulation of lysyl oxidase . Biochim. Biophys. Acta. 2003. 1647. P. 239-244. CrossRef  
  19. Harris E.D., Gonnerman W.A., Savage J.T. Connective tissue amine oxydase. Purification and partial characte­rization of lysyl oxydase from chick aorta . Ibid. 1974. 341. P. 332-344. CrossRef  
  20. Li W., Liu G., Chou I.N., Kagan H.M. Hydrogen per­oxide-mediated, lysyl oxidase-dependent chemotaxis of vascular smooth muscle cells . J. Cell Biochem. 2000. 78. P. 550-557. CrossRef  
  21. Maki J.M, Sormunen R., Lippo S., Kaarteenaho-Wiik R., Soininen R., Myllyharju J. Lysyl Oxidase Is Essential for Normal Development and Function of the Respiratory System and for the Integrity of Elastic and Collagen Fi­bers in Various Tissues . Amer. J. Pathol. 2005. 167, N 4. P.927-936. CrossRef  
  22. Maki J.M. Lysyl oxidases in mammalian development and certain pathological conditions . Histol Histo-pathol. 2009. 24(5). P.651-660.
  24. Maranghi F., Tassinari R., Lagatta V. Effects of the food contaminant semicarbazide following oral admin­istration in juvenile Sprague-Dawley rats . Food and Chem.Tox. 2009. 47. P.472-479. CrossRef PubMed
  26. Kusama-Eguchi K.., Ikegami F., Kusama T., Suda A., Ogawa Y., Igarashi K., Watanabe K. A rat model of neurolathyrism: repeated injection of beta-ODAP in­duces the paraparesis of the hind legs . Amino Acids. 2005. 28(2). P.139-143. CrossRef PubMed
  28. Palamakumbura A.H., Trackman PC. A Fluorometric as­say for detection of lysyl oxidase enzyme activity in biological samples . Anal. Biochem. 2002. 300. P.245-251. CrossRef PubMed
  30. Postovit L.M., Abbott D.E., Payne S.L., Wheaton W.W., Margaryan V. Hypoxia. reoxygenation: a dynamic regulator of lysyl oxidase-facilitated breast cancer migration . J. Cell Biochem. 2008. 103. P.1369-1378. CrossRef PubMed
  32. Szarvas T., Szatloczky E., Volford J. Determination of endogenous formaldehyde level in human blood and urine by dimedone-14C radiometric . J. Radioanal. and Nucl. Chem. 1986. 106(6). P. 357-367. CrossRef  

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