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Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(3): 40-48

The influence of calcitonin on the liver secretory function in rats

Horenko ZA, Karabovs'ka LS, Vasheka IP, Vesel's'kyĭ SP

    Taras Shevchenko National University of Kyiv, Ukraine


The influence of calcitonin on the choleresis level and bile acids spectrum was investigated in acute experiments on the rats with common biliary duct cannulated. We determined the intensity of bile secretion during 3 hours and quantitative content of conjugated and free bile acids using thin-layer chromatography. It was shown that intramuscular introduc­tion of synthetic salmon calcitonin (200 ng/kg) increases the secreted bile volume and the content of conjugated bile acids in the bile. The administration of salmon calcitonin resulted in increase of bile volume (+17,4% (P<0.05)), taurotrihyd-roxycholates (+58,3% (P<.001)), taurodihydroxycholates (+68,5% (P<0.01)), glycodihydroxycholates (+120,8% (P<0.01)), while the total content of free bile acids was not significantly altered as compared with control. At the same time, under the hormone influence we observed bile acid spec­trum redistribution and a part of conjugated cholates increased against a decreasing of free bile acids part. The present results suggest that the salmon calcitonin intensifies the processes of conjugation of bile acids with amino acids taurine and glycine in hepatocytes and canalicular secretion that results in improvement of solubilization properties of the bile, ability of the bile to hold cholesterol in solute state and prevent the formation of calculi in biliary tracts.

Keywords: calcitonin, bile formation, taurocholates,glycocholates, free bile acids.


  1. A.c. 1624322 USSR, MBI G 01 N 33. 50 Method of Determination of Bile Acids in Biological Liquid . SP. Veselsky, PS Lyashchenko, I.A. Lukyanenko (USSR); N 4411066. 14; claimed 01. 25. 1988; Published January 30, 1991, Bul.No. 4.
  3. Andreotti G., Mendez B.L., Amodeo P., Morelli M.,Nakamuta H., Motta A. Structural determinants of salmon calcitonin bioactivity. The role of the leu-based amphipathic b-helix . J. Biol. Chem. 2006. 281, N 34. P.24193-24203. CrossRef PubMed
  5. Azria M. Possible mechanisms of the analgesic action of calcitonin . Bone. 2002. 30, N 5. P.80S-83S. CrossRef  
  6. Bello N.T., Kemm M.H., Moran T.H. Salmon calcitonin reduces food intake through changes in meal sizes in male rhesus monkeys . Amer. J. Physiol. Regul. Integr. Comp. Physiol. 2008. 295. P.R76-R81. CrossRef PubMed PubMedCentral
  8. Bracq S., Machairas M., Clement B. Pidoux E., Andreoletti M., Moukhtar M.S., Jullienne A. Calcitonin gene expression in normal human liver . FEBS Lett. 1993. 331. P.15-18. CrossRef  
  9. Chen S., Morimoto S., Tamatani M., Fukuo K., Nakahashi T., Nishibe A., Jiang B., Ogihara T. Calcitonin prevents CCl induced hydroperoxide gen-eration and cytotoxicity possibly through C1b receptor in rat hepatocytes . Boichem. Biophys. Res. Com. 1996. 218. P.865-71. CrossRef PubMed
  11. Davey R.A., Turner A.G., McManus J.F., Chiu W.S., Tjahyono F., Moore A.J., Atkins G.J., Anderson P.H., Ma C, Glatt V., MacLean H.E., Vincent C, Bouxsein M., Morris H.A., Findlay D.M., Zajac J.D. Calcitonin receptor plays a physiological role to protect against hypercalcemia in mice . J. Bone Miner. Res. 2008. 23 (8). P.1182-1193. CrossRef PubMed PubMedCentral
  13. Dubay D., Ephgrave K.S., Cullen J.J., Broadhurst K.A. Intracerebroventricular calcitonin prevents stress-in­duced gastric dysfunction . J. Surg. Res. 2003. 119. P.188-192. CrossRef  
  14. Eiden S., Daniel C, Steinbrueck A. Schmidt I., Simon E. Salmon calcitonin a potent inhibitor of food intake in states of impaired leptin signalling in laboratory rodents . J. Physiol. 2002. 541 (3). P.1041-1048. CrossRef PubMed PubMedCentral
  16. Franceschini R., Cataldi A., Cianciosi P., Garibaldi A., Consini G., Barreca T., Rolandi E. Calcitonin and v-endorphin secretion . Biomed. Pharmacother. 1993. 47. P.305-309. CrossRef  
  17. Gatmaitan Z.C., Nies A.T., Arias I.M. Regulation and translocation of ATP-dependent apical membrane pro­teins in rat liver . Amer. J. Physiol. Gastrointest. Liver Physiol. 1997. 272. P.G1041-G1049. CrossRef PubMed
  19. Hilton M.J., Dowton M., Houssami S., Sexton P.M. Identification of key components in the irreversibility of salmon calcitonin binding to calcitonin receptors . J. Endocrinol. 2000. 166. P.213-226. CrossRef PubMed
  21. Hotz J., Goebell H., Ziegler R. Calcitonin and exocrine pancreatic secretion in man: inhibition of enzymes stimulated by CCK-pancreozymin, caerulein, or calcium no response to vagal stimulation . Gut. 1977. 18. P.615-622. CrossRef PubMed PubMedCentral
  23. Jonderko K., Bueno L. Effect of peripherally and cen­trally administered calcitonin on gallbladder emptying in dogs . J. Gastroenterol. 1997. 32. P.380-388. CrossRef PubMed
  25. Kanis J., McCloskey E. Effect of calcitonin on verte­bral and other fracture . Q. J. Med. 1999. 92. P.143-150. CrossRef PubMed
  27. Krzysik-Walker S.M., Ocon-Grove O.M., Maddineni S.B., Hendricks III G.L., Ramachandran R.. Identifica­tion of calcitonin expression in the chicken ovary: influence of follicular maturation and ovarian steroids . Biol. Reprod. 2007. 77. P.626-635. CrossRef PubMed
  29. Kubitz R., Sutfels G., Kuhlkamp T., Kolling R., Haussinger D. Trafficking of the bile salt export pump from the Colgi to the canalicular membrane is regulated by the p38 MAP kinase . Gastroenterology. 2004. 126. P.407-419. CrossRef PubMed
  31. Kullak-Ublick G.A., Stieger B., Meier P.G. Enterohepatic bile salt transporters in normal physiology and liver dis­ease . Ibid. 2004. 126 (1). P.322-342. CrossRef PubMed
  33. Li Q., Wang J., Armant R., Bagchi M.K., Bagchi I.C. Calcitonin down regulates E-cadherin expression in rodent uterine epithelium during implantation . J. Biol. Chem. 2002. 277, N 48. P.46447-46455. CrossRef PubMed
  35. Lutz T.A., Tschudy S., Rushing P.A., Scharrer E. Amylin receptors mediate the anorectic action of salmon calcitonin (sCT) . Peptides. 2000. 21. P.233-238. 21.Menta N.M., Malootian A., Gilligan J.P. Calcitonin for osteoporosis and bone pain . Curr. Pharm. Des. 2003. 9. P.2659-2676. CrossRef  
  36. Nishizawa Y., Okui Y., Inaka M., Yukicka K., Miki T., Watanabe Y, Morii H. Calcium. calmodulin-mediated action of calcitonin on lipid metabolism in rats . J. Clin. Invest. 1988. 82. P.1165-1172. CrossRef PubMed PubMedCentral
  38. Pham V., Wade J.D., Purdue B.W., Sexton P.M. Spatial proximity between a photolabile residue in position 19 of salmon calcitonin and the amino terminus of the human calcitonin receptor . J. Biol. Chem. 2004. 279,N 8. P.6720-6729. CrossRef PubMed
  40. Poyner D.R., Sexton P.M., Marshall I., Smith D.M., Quirion R., Born W., Muff R., Fisher J., Foord S.M. International union of pharmacology. XXXII. The mammalian calcitonin gene-related peptides, adreno-medullin, amylin, and calcitonin receptors . Pharmacol. Rev. 2002. 54. P.233-246. CrossRef PubMed
  42. Purdue B., Tilakaratne N., Sexton P.M. Molecular pharmacology of the calcitonin receptor . Recept. Cennels. 2002. 8 (3-4). P.243-255. CrossRef PubMed
  44. Ren Y, Chien J., Sun Y.P., Shan G.V. Calcitonin is ex­pressed in gonadotropes of the anterior pituitaty gland: its possible role in paracrine regulation of lactotrope function . J. Endocrinol. 2001. 171. P.217-228. CrossRef PubMed
  46. Schonhoff C.M., Webster C.R.L., Anwer M.S. Cyclic AMP stimulates Mrp2 translocation by activating p386 MAPK in hepatic cells . Amer. J. Physiol. Gastrointest. Liver Physiol. 2010. 298. P.G667-G674. CrossRef PubMed PubMedCentral
  48. Seema M., Sushima B., Ranjan J. Evaluation of the analgesic effect of salmon calcitonin in metastatic bone pain . Unit Anaesthesiol. 2003. 9 (1). P.8-13.
  50. Shah G.V., Rayford W., Noble M.J., Austenfeld M., Weidel J., Vamos Mebust W.K. Calcitonin stimulates growth of human prostate cancer cells through receptor-mediated increase in cyclic adenosine 3',5'-monophos-phates and cytoplasmic Ca2+ transient . Endocrinol­ogy. 1994. 134. P.596-602. CrossRef PubMed
  52. Sommerville B.A., Fox J. Changes in renal function of the chicken associated with calcitonin and parathyroid hormone . Gen. Comp. Endocrinol. 1987. 66. P.381-386. CrossRef  
  53. Tarnawski A., Bogdal J., Dura K., Marszalek Z., Jedrychowski A. Effect of calcitonin on the formation, composition, and enzymatic activity of the hepatic bile in guinea pigs . Gut. 1974. 15, N 9. P.703-705. CrossRef PubMed PubMedCentral
  55. Walters H.C., Craddock A.L., Fusegawa H., Willingham M.C., Dawson P.A. Expression, transport properties, and chromosomal location of organic anion transporter subtype 3 . Amer. J. Physiol. Gastrointest. Liver Physiol. 2000. 279. P.G1188-G1200. CrossRef PubMed
  57. Watanabe S., Phillips M.J. Ca2+ causes active contraction of bile canaliculi: direct evidence from mi-croinjection studies . Proc. Natl. Acad. Sci. USA. 1984. 81. P.6164-6168. CrossRef PubMed PubMedCentral
  59. Yamaguchi M. Calcitonin stimulates gluconeogenesis in fasted rats . Endocrinol. Jpn. 1981. 28. P.51-57. CrossRef PubMed
  61. Yamaguchi M. Stimulatory effect of calcitonin on Ca2+ inflow in isolated rat hepatocytes . Mol. Cell. Endocrinol. 1991. 75 (1). P.65-70. CrossRef  

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