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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(3): 25-32


Diabetes-evokedchanges in Na2+-dependent potassium conductance of isolated smooth muscle cells of the ratbladder

D.A. Erishtal, I.M. Paduraru, I... Boldyrev, I.Iu. E.t, V.V. Rekalov Ia.M. Shuba.

    Ін-т фізіології ім. О.О. Богомольця НАН України, Київ;Міжнарод. центр молекуляр. фізіології НАН України, Київ


Abstract

Activation of large conductance Ca2+-dependent potassium channels (BK channels) influences repolarization of the action potential and the level of the resting potential of detrusor smooth muscle cells (SMC). Overactive bladder syndrome (OBS) is one of the complications of diabetes. Here using whole-cell patch clamp technique we show sizable reduction of depolarization-evoked BK current (T) and decrease in the amplitude and frequency of spontaneous transient outward currents (STOCs) in isolated SMC from detrusor of rats with streptozocin-induced diabetes compared to control animals. Under the diabetes L_ density at step depolarization to +50 mV decreased from control value of 15.0±0.4 pA/pF to 10.0і0.5 pA/pF, whereas the mean values of the STOCs’ frequency and amplitude at holding potential -20 mV were reduced from 12.0±1.5 Hz to 2.4±0.6 Hz and from 0.9±0.1 pA/pF to 0.5±0.1 pA/pF, respectively. Using real time RT-PCR it was found that the expression of mRNA for the BK-channel primary pore-forming KCa 1.1-subunit increases under the diabetes, whilst that for the auxiliary BKC (31-subunit de­creases. It is concluded that the observed changes in the BK-channel currents can enhance excitability of the detrusor SMCs thereby promoting myogenic OBS. However, further studies are needed to determine how the decrease in BK p1 expres­sion under the diabetes impairs functional properties of BK channels and to establish possible changes in calcium signals that modulate channel activation.

Keywords: bladder, detrusor, smooth muscles, diabetes mel-litus, calcium dependent potassium channels, BK-channels

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