Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(3): 3-9

The mitochondrial membrane potential and oxygen consumption in the heart of spontaneously hypertensive rats

Dorofeieva NO, Hoshovs'ka IuV, Sahach VF

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz57.03.003


Abstract

We investigated the mitochondrial membrane potential and processes of respiration and oxidative phosphorylation in sus­pension of cardiac mitochondria from 6 month old spontane­ously hypertensive rats (SHR) and Wistar (as a control) male rats. The mitochondrial membrane potential and the speed of oxygen consumption were measured using the method de­scribed by M.Brand (1995). Processes of respiration and oxi­dative phosphorylation in cardiac mitochondria were measured using Oxygraph (Hansatech instruments, Norfolk, England). It has been found that in SHR the mitochondrial membrane potential was lower (-113,76mV ±3,65 mV) compared to Wistar rats (AV = -152,85 mV±13,52mV, p<0,01). In SHR, the respiration rate in state V2 by Chance and V3 were increased compared to Wistar rats (p<0,001). The respiration control by Chance was depressed by 23,9% in SHR compared to Wistar rats. Our data demonstrate, that SHR have some features of functioning of cardiac mitochondria which distin­guish them from the Wistar rats. Our data suggest a functional link between mitochondrial energy supply and arterial hyper­tension.

Keywords: mitochondria, hypertension, membrane potential.

Full text: (PDF, in Ukrainian)

References

  1. Budnikov E.Yu., Postnov A.Yu., Doroshchuk A.D., Afanasyeva G.V., Postnov Yu.V. Reduced ATP-synthesizing ability of spontaneously hypertensive rat mitochondria of liver (SHR): the role of calcium overload of mitochondria . Cardiology. 2002. N 12. WITH. 47-50.
    2.  
  2. Gogin E.E. Arterial hypertension syndrome as a sign of maladaptation disorders . Klin. medicine. 2002. N 11. p. 4-9.
    3.  
  3. Doroshchuk AD, Postnov A.Yu., Afanasyeva GV, Budnikov EY, Postnov YV Reduced ATP-synthesizing ability of rat brain mitochondria with spontaneous hypertension (SHR) . Cardiology. 2004. N 3. P. 64-65.
    4.  
  4. Emelina LP Some phosphorus-energy parameters of blood in patients with hypertension . Doctor. business. 1972. N 8. p. 10-13.
    5.  
  5. Kosterin S.A., Bratkova N.F., Kursky M.D. The role of sarcolemma and mitochondria in providing calcium control of myometrial relaxation . Biochemistry. 1985. 50, N 8. P. 1350-1361.
    6.  
  6. Lukyanova LD Bioenergy hypoxia: concept, mechanisms and methods of correction . BEBiM. 1997. 124, N 9. P. 244-254.
    7.  
  7. Nagornaya NV, Pshenichna EV, Bordyugova EV Hypertension in children. Modern approaches to diagnostics, treatment, prevention . Tavr. medical-biol. news 2009. 12, N 2 (46). P.105-111.
    8.  
  8. Pisarenko OP, Studneva IM, Postnov A.Yu. Peculiarities of the energy state of tissues in spontaneous rat hypertension . Cardiology. 1998. 12, N 37. p. 37-40.
    9.  
  9. Postnov Yu.V. On the development of the membrane concept of pathogenesis of primary hypertension: impaired mitochondrial function and energy deficiency . Cardiology. 2000. N 10. p. 4-12.
    10.  
  10. Postnov Yu.V., Orlov SN, Budnikov E.Yu., Doroshchuk AD, Postnov A.Yu. Disruption of energy conversion in cell mitochondria with a decrease in ATP synthesis as a cause of steady increase in systemic blood pressure . Cardiology. 2008. N 8. P. 49-59.
    11.  
  11. Tkachenko GM, Kurgalyuk NM Influence of KAT-channel activator pinacidin on functioning of rat liver mitochondria with different resistance to hypoxia under stress . Ukr. biochem. . 2004. 76, N 1. P. 56-64.
    12.  
  12. Fomin IG, Dyakova TA Left ventricular hypertrophy in arterial hypertension and risk of arrhythmias . Cardiovas. therapy and prevention. 2006. N 5 (8). P. 83.
    13.  
  13. Chernobrivenko AA Angiotensin-converting enzyme inhibitors and hypertension . News of medicine and pharmacy. 2005. N 16. P.13-14.
    14.  
  14. Brand M.D. Measurement of mitochondrial proton-motive force. Bioenergetics: A Pactical Approach, IRL Press, Oxford. 1995. P. 39-62.
    15.  
  15. Chance B.,Williams G. The respiratory Chain and Oxydative Phosphorylation .Adv. Enzymol. 1956. 17. P. 65-134. CrossRef PubMed
    16.  
  16. Duchen Michael R. Mitochondria in health and disease: perspectives on a new mitochondrial biology . Mol. Aspects Med. 2004. 25. P. 365-451. CrossRef PubMed
    17.  
  17. Lowry O.H., Rosenbrough N.J., Farr A.L. Protein measurement with the Folling phenol reagent . J. Biol. Chem. 1951. 193, N 1. P. 265-275.
    18.  
  18. Nadtochiy S.M., Tompkins A., Brookes P.S. Different mechanisms of mitochondrial proton leak in ischaemia. reperfusion injury and precondition: implications for pathology ancardioprotection . Biochem J. 2006. 395. P. 611-618. CrossRef PubMed PubMedCentral
    19.  
  19. Nicholls D.G. Mitochondrial membrane potential and aging . Aging Cell. 2004. N 3. P. 35-40. CrossRef PubMed
    20.  
  20. Resnick L.M., Gupta R.K., Barbagallo M., Laragh J.H. Is the higher incidence of ischemic disease in patients with hypertension and diabetes related to introcellular depletion of high energy metabolites?. Amer. J. Med. Sci. 1994. 307: Suppl 1. P. 66-69.
    21.  
  21. Ronquist G., Soussi D., Frithz G. Disturbed energy bal­ance in skeletal muscle of patients with untreated prima­ry hypertension . J. Intern. Med. 1995. 238. P.167-174. CrossRef PubMed
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.