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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(3): 3-9

The mitochondrial membrane potential and oxygen consumption in the heart of spontaneously hypertensive rats

Dorofeieva NO, Hoshovs'ka IuV, Sahach VF

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


We investigated the mitochondrial membrane potential and processes of respiration and oxidative phosphorylation in sus­pension of cardiac mitochondria from 6 month old spontane­ously hypertensive rats (SHR) and Wistar (as a control) male rats. The mitochondrial membrane potential and the speed of oxygen consumption were measured using the method de­scribed by M.Brand (1995). Processes of respiration and oxi­dative phosphorylation in cardiac mitochondria were measured using Oxygraph (Hansatech instruments, Norfolk, England). It has been found that in SHR the mitochondrial membrane potential was lower (-113,76mV ±3,65 mV) compared to Wistar rats (AV = -152,85 mV±13,52mV, p<0,01). In SHR, the respiration rate in state V2 by Chance and V3 were increased compared to Wistar rats (p<0,001). The respiration control by Chance was depressed by 23,9% in SHR compared to Wistar rats. Our data demonstrate, that SHR have some features of functioning of cardiac mitochondria which distin­guish them from the Wistar rats. Our data suggest a functional link between mitochondrial energy supply and arterial hyper­tension.

Keywords: mitochondria, hypertension, membrane potential.


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