Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(1): 72-81

Disorders of uric acid metabolism in rats with fructose-induced experimental insulin resistance syndrome

Shuprovych AA, Hurina NM, Korpacheva-Zinych OV

    State Institution V.P. Komissarenko Institute of Endocrinology and Metabolism of National Àñademy of Medical Sciences, Kyiv, Ukraine


Consumption of dietary fructose has been recently suggested to be one of the environmental factors contributing to develop­ment of obesity and accompanying abnormalities of the insulin resistance syndrome (IRS). IRS was induced in rats by adding 10% fructose to drinking water. Within 8 wk insulin resistance, carbohydrate metabolism disorder, weight gain, hyper-trigyceridemia and hyperuricemia were developed. The signs of IRS were more pronounced in male than in female rats. An increase of uric acid (UA) daily excretion was revealed only in males, suggesting a hyperproductoin of UA. There was not significant disorder of kidney filtration, although an increase of UA clearance was revealed due to enhanced kidney urate reabsorbtion which may be connected to the hypouricosuric action of insulin but not the testosterone level changes. It was first revealed an increased activity of enzymes catalyzing early stages of purine catabolism (5'-nucleotidase and adeno-sindesaminase) in liver homogenate. Activity of enzyme of purine reutilisatoin (hypoxantin-guanine-phosphoribosil trans­ferase) was found increased that may have a compensatory significance under conditions of increased purine degradation.

Keywords: uric acid, insulin resistance syndrome, fructosediet, rats, purine degradation enzymes.


  1. Preclinical drug studies (guidelines) . Ed. OV Stefanova. K .: Avicenna. 2001. 528 p.
  3. Madanov IV, Balabolkin MI, Markov DS, Markova TN The main causes of hyperuricemia in diabetes mellitus . Therap. archive. 2000, N 2. pp. 58-60. CrossRef  
  4. Mukhin NA, Balkarov IM, Britov AN Tubulo-interstitial nephritis and hypertension clinical and population value . Therap. archive. 1997. 69, N 7. p. 5-10.
  6. Manual of Clinical Laboratory Diagnosis . Ed. Menshikova VV. M .: Medicine, 1982. 576 p.
  8. Magarlamov A.G. Asparaginase and adenosine-deaminase activity in rat liver tissue under different conditions of protein and protein-free nutrition . Ukr. biochem. . 1980. 52, N 4. p. 446-452.
  10. Bantle J.P., Raatz S.K., Thomas W., Georgopoulos A. Effects of dietary fructose on plasma lipids in healthy subjects . Amer. J. Clin. Nutr. 2000. 72. P. 1128-1134. CrossRef PubMed
  12. Dai S., McNeill J.H. Fructose-induced hypertension in rats is concentration- and duration-dependent . J. Pharmacol. Toxicol. Methods. 1995. 33. P. 101-107. CrossRef  
  13. Dennison B.A., Rockwell H.L., Baker S.L. Excess fruit juice consumption by preschool-aged children is asso­ciated with short stature and obesity . Pediatrics. 1997. 99. P. 15-22.
  15. 9. Facchini F., Chen Y.D., Hollenbeck C.B., Reaven G.M. Relationship between resistance to insulin-mediated glu­cose uptake, urinary uric acid clearance, and plasma uric acid concentration . JAMA. 1991. 266. P. 3008-3011. CrossRef PubMed
  17. 10. Galipeau D., Verma S., McNeill J. Female rats are pro­tected against fructose-induced changes in metabolism and blood pressure . Amer. J. Physiol. Heart. Circ. Physiol. 2002. 283, N 6. P. H2478-H2484. CrossRef PubMed
  19. Hwang I.S., Ho H., Hoffman B.B., Reaven G.M. Fruc­tose-induced insulin resistance and hypertension in rats . Hypertension. 1987. 10. P. 512-516. CrossRef PubMed
  21. Ludwig D.S., Peterson K.E., Gortmaker S.L. Relation between consumption of sugar-sweetened drinks and childhood obesity: a prospective, observational analysis. Lancet. 2001. 357. P. 505-508. CrossRef  
  22. Masuo K., Kawaguchi H., Mikami H., Ogihara T., Tuck M.L. Serum uric acid and plasma norepinephrine concentrations predict subsequent weight gain and blood pressure elevation . Hypertension. 2003. 42. P. 474-480. CrossRef PubMed
  24. Michel R.M., Hawthorne S. N. The site of diphos-phoinozitole synthesis in rat liver . Biochem. Biophys. Res. Commun. 1965. 21, N 4. P. 333-338. CrossRef  
  25. Nakagawa T., Hanbo Hu, Zharikov S., Tuttle K.R., Short R.A., Glushakova O., Xiaosen Ouyang, Feig D.I., Block E. R., Herrera-Acosta J., Patel J. M., Johnson R. J. A causal role for uric acid in fructose-induced metabolic syndrome . Amer. J.Physiol. Renal. Physiol. 2006. 290. P. F625-F631. CrossRef PubMed
  27. Pizzichini M., Leoncini R., Vannoni D. The influence of testosterone on purine nucleotide metabolism in rat liver . Life Sci. 1995. 57. P. 2127-2135. CrossRef  
  28. Sanchez-Lozada L., Tapia E., Jimnez A. Fructose-in­duced metabolic syndrome is associated with glom­erular hypertension and renal microvascular damage in rats . Amer. J. Physiol. Renal Physiol. 2006.- 292. P. F423-F429. CrossRef PubMed
  30. Hare M.B., Manson J.E., Ludwig D.S. Sugar-sweet­ened beverages, weight gain, and incidence of type 2 diabetes in young and middle-aged women . JAMA. 2004. 292. P. 927-934. CrossRef PubMed
  32. 19. Sekine T., Miyazaki H., Endou H. Molecular physiol­ogy of renal organic anion transporters . Amer. J. Physiol. Renal Physiol. 2006. 290. P. F251-F261. CrossRef PubMed
  34. 20. Song D., Arikawa E., Galipeau D., Battell M., McNeill J.H. Androgens are necessary for the development of fructose-induced hypertension . Hypertension. 2004. 43. P. 667-672. CrossRef PubMed
  36. Stirpe F., Della Corte E., Bonetti E. Fructose-induced hyperuricaemia . Lancet. 1970. N 2. P. 1310-1311. CrossRef  
  37. Tsunoda S., Kamide K., Minami J., Kawano Y. De­creases in serum uric acid by amelioration of insulin resistance in overweight hypertensive patients: effect of a low-energy diet and an insulin-sensitizing agent . . Amer. J. Hypertens. 2002. 15. P. 697-701. CrossRef  
  38. Vasudevan H., Xiang H., McNeill J.H. Differential regulation of insulin resistance and hypertension by sex hormones in fructose-fed male rats . Amer. J. Physiol. Heart Circ. Physiol. 2005. 289. P. H1335-H1342. CrossRef PubMed
  40. Vizzotto L., Vartemati M., Marinello E., Leoncini R., Pagani R., Pizzichini M. Effect of testosterone on purine metabolism and morphometric parameters in the rat liver . . Mol. Cell. Endocrinol. 1996. 119. P. 123-127. CrossRef  
  41. Xiang G., Lu Qi, Qiao N. Intake of added sugar and sugar-sweetened drink and serum uric acid concent­ration in US men and women . Hypertension. 2007. 50. P. 306-316. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.