Functional activity of monocytes and mechanisms of iNOS intracellular regulation during wound process
Barinova ME, Iel's'kyĭ VM, Barinov EF, Sulaieva OM
M. Gorky Donetsk National Medical University, Ukraine
DOI: https://doi.org/10.15407/fz57.01.036
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Abstract
To investigate the dynamics and mechanisms of iNOS activity during wound process, the peripheral blood monocytes of 22 patients with acute foot wounds were analyzed. The basal and LPS-stimulated nitrites production was estimated at 1, 3-4, 7-10 and 14-18 days of wound process. iNOS activity and its molecular regulation was estimated by the inhibitory analysis. It was shown that since 1st to 3-4 day of wound process the basal and LPS-stimulated activity of iNOS, PDE and 5-LOX were elevated initially and than decreased. The COX and PkC activities increased after 3 days and reached the maximum level at days 7-10. The activity of PkA, which inhibits iNOS, intensively increased form 7-10th to 14-18th days of healing, and was accompanied by arginase-1 stimulation. Thus monitoring of intracellular signaling system can be used for diagnostics and correction of wound healing.
Keywords:
wound healing, monocytes-macrophages, iNOS.
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