Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(1): 29-35

The immunophenotypic characteristics of two functionally different natural killer cell subpopulations in peripheral human blood

Donskoĭ BV, Chernyshov VP, Osypchuk DV

    Insitute of Pediatrics, Obstetrics and Gynecology of AMS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz57.01.029


Abstract

NK cell cytotoxicity and immunophenotypic characteristics of activated natural killer (NK) cells after co-incubation with K562 target cells in women were investigated. An increase in CD69, HLADR, CD95 expression on target-activated NK cells was demonstrated. Conversely, CD62L expression in NK cells after activation was decreased. The results showed that only a part of NK cells after incubation with K562, became CD69 positive. Moreover, even after lowering of “efector - target ” ratio and extensions of time of stimulation, the amount of CD69+NK cells did not exceed 60% (50%±1.7%). Expres­sion of CD69, CD95 and HLADR on NK cells before co-incubation didn`t correlate with NK cytotoxicity. However, size of population of activated CD69+ NK cells (after stimu­lation) was correlated with NK cytotoxicity. We also found that the activation capacity after cocultivation with K562 cells is related to loss in CD62L on NK cell`s surface. Only CD62Lneg and less CD62LlowNK cells had the ability to be activated with K562. In conclusion, we demonstrated that incubation with K562 target cells enhanced the expression of CD69 on the major part of NK cell population and the size of CD69+NK cell population is strongly correlated with NK cytotoxicity.

Keywords: natural killer cells, markers of activation, NKcell‘s cytotoxicity.

Full text: (PDF, in Ukrainian)

References

  1. Benlahrech F.,Donaghy H., Rozis G., Goodier M., Klavinskis L., Gotch F., Patterson S. Human NK cell Up-regulation of CD69,HLA-DR, Interferon g Secre­tion and Cytotoxic Activity by Plasmocytoid Dendritic Cells is Regulated through Overlapping but Different Pathways.Sensors. 2009. 6. P.386-403. CrossRef PubMed PubMedCentral
    2.  
  2. Chalifour A., Jeannin P., Gauchat J- F.,Blaecke A.,Malissard M., N'Guyen T., Thieblemont N., Delneste Y. Direct bacterial protein PAMP recognition by human NK cells involves TLRs and triggers 6-defensin production . Blood. 2004. 104, N 6. P.1778-1783. CrossRef PubMed
    3.  
  3. Caligiuri M.A. Human natural killer cells.Ibid. 2008. 112,N 3. P.461-469. CrossRef PubMed PubMedCentral
    4.  
  4. Yun C.H., Lundgren A., Azem J., Sloling A., Holmgren J., Svennerholm A. M.,Lundin B.S. Natural killer cells and Helicobacter pylori infection: bacterial antigens and interleukin-12 act synergistically to induce gamma interferon production. Infect.Immun. 2005. 73, N 3. P.1428-1490. CrossRef PubMed PubMedCentral
    5.  
  5. Dosiou C, Giudice L.C. Natural killer cells in preg­nancy and recurrent pregnancy loss: endocrine and immunologic perspectives.Endocrine Rev. 2005. 26,N 1. P.44-62. CrossRef PubMed
    6.  
  6. Eriksson.M., Meadow S.K., Basu S., Mselle T.F., Wira C.R., Sentman C.L.. TLR Mediate IFN-g production by human uterine NK cells in endometrium. J.Immunol. 2006. 176. P.6219-6224. CrossRef PubMed
    7.  
  7. Young H.A., Ortaldo J. Cytokines as critical co-stimu­latory molecules in modulating immune response of natural killer cells. Cell Res. 2006. 16. P.16-24. CrossRef PubMed
    8.  
  8. Kogure T., Mantani N., Sakai S., Shimada Y, Tamura J., Terasawa K. Natural killer cytolytic activity is as­sociated with the expression of killer cell immuno-globulin-like receptors on peripheral lymphocytes in human. Med. Inflam. 2003. 12. P.117-121. CrossRef PubMed PubMedCentral
    9.  
  9. Kumar V., Medhi B. Emerging role of uterine natural killer cells in establishing pregnancy.Iran. J.Immunol. 2008. 5,N 2. P.71-81.
    10.  
  10. Zamai L., Ponti C, Mirandola P.,Gobbi G., Papa S.,Galeotti., Cocco L., Vitale M. NK cells and cancer. J. Immunol. 2007. 178. P.4011-4016. CrossRef PubMed
    11.  
  11. Ntrivalas E.I., Kwak-Kim J.Y.H., Gilman-Sachs A., Chung-Bang H., Ng S.CBeaman K.D., Mantouvalos H.P., Beer A.E. Status of peripheral blood natural killer cells in women with recurrent spontaneous abortions and infertility of unknown aetiology.Hum. Rreprod. 2001. 16,N 5. P.855-861. CrossRef PubMed
    12.  
  12. Redline R.W. Role of uterine natural killer cells and interferon g in placental development.J.Exp. Med. 2000. 17, N 2. P.1-4. CrossRef PubMed PubMedCentral
    13.  
  13. Farag S.S., Fehniger T.A., Ruggeri L., Caliguri M.A. Natural killer cell receptors: new biology and insights into the graft- versus-leukemia effect.Blood. 2002. 100,N 6. P.1935-1947. CrossRef PubMed
    14.  
  14. Chen S., Kawashima H., Lowe J.B., Lanier L.L.,Fukuda M. Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment. J.Exp.Med. 2005. 202, N 12. P.1679-1689. CrossRef PubMed PubMedCentral
    15.  
  15. Sun J.C., Lanier L.L. Natural killer cells remember: An evolutionary bridge between innate and adaptive immu­nity?. Eur. J. Immunol. 2009. N 39. P.2059-2064. CrossRef PubMed PubMedCentral
    16.  
  16. Warren H.S, Campbell A.J., Waldron J.C, Lanier L.L. Biphasic response expressing both activating and in­hibitory killer Ig-like.Intern.Immunol. 2001. 13, N 8. P.1043-1052. CrossRef PubMed
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.