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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(1): 29-35

The immunophenotypic characteristics of two functionally different natural killer cell subpopulations in peripheral human blood

Donskoĭ BV, Chernyshov VP, Osypchuk DV

    Insitute of Pediatrics, Obstetrics and Gynecology of AMS of Ukraine, Kyiv, Ukraine


NK cell cytotoxicity and immunophenotypic characteristics of activated natural killer (NK) cells after co-incubation with K562 target cells in women were investigated. An increase in CD69, HLADR, CD95 expression on target-activated NK cells was demonstrated. Conversely, CD62L expression in NK cells after activation was decreased. The results showed that only a part of NK cells after incubation with K562, became CD69 positive. Moreover, even after lowering of “efector - target ” ratio and extensions of time of stimulation, the amount of CD69+NK cells did not exceed 60% (50%±1.7%). Expres­sion of CD69, CD95 and HLADR on NK cells before co-incubation didn`t correlate with NK cytotoxicity. However, size of population of activated CD69+ NK cells (after stimu­lation) was correlated with NK cytotoxicity. We also found that the activation capacity after cocultivation with K562 cells is related to loss in CD62L on NK cell`s surface. Only CD62Lneg and less CD62LlowNK cells had the ability to be activated with K562. In conclusion, we demonstrated that incubation with K562 target cells enhanced the expression of CD69 on the major part of NK cell population and the size of CD69+NK cell population is strongly correlated with NK cytotoxicity.

Keywords: natural killer cells, markers of activation, NKcell‘s cytotoxicity.


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