Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(1): 3-8

Calcium signaling induced by tonic firing in the rat eye retinal ganglion cells

Kuznietsov KI, Maslov VIu, Fedulova SA, Veselovs'kyĭ MS.

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine


On rat retinal preparation in whole-cell mode retinal ganglion cells (RGC) tonic firing induced calcium signals were recorded and their modulation by Kv3 potassium channels was studied. RGC depolarization with 500 ms current steps caused tonic action potentials (AP) generation and development of calcium signals which amplitude depended quite linearly on mean AP frequency with mean slope 1.4 ± 0.2 s-nmol/l (n = 5). The calcium signals mean tau of decay value was 6.8 ± 1.4 s (n = 5). After application of tetraethylammoniun (500 мM), which in such concentration blocks Kv3.1/Kv3.2 potassium channels, a decrease in firing frequency, an increase of AP width and a decrease of afterhyperpolarization amplitude were observed. In the presence of the blocker the calcium signal amplitude vs mean AP frequency dependency slope mean value increased to 2.1 ± 0.3 s-nmol/l. The calcium signals tan of decay not changed significantly, the mean value was 8.9 ± 1.6 s (n = 5). Thus, voltage-dependent Kv3.1/Kv3.2 potassium channels, which play a key role in tonic firing pattern generation in RGC, also modulate appropriate calcium signals and prevent the cells from significant intracellular calcium increasing and its possible cytotoxic action.

Keywords: retinal ganglion cells, tonic firing, calcium signals,Kv3 potassium channels


  1. Veselovskaya ZF, Veselovskaya NN Comparative efficacy of norvask and amlodipine on the results of dynamic changes in objective indicators of the functional state of the macular area of the retina in patients with open-angle glaucoma and non-proliferative diabetic retinopathy without diabetic macular edema . Nauk. hanging nat. honey. to them. OO Bogomolets. Topical issues of glaucoma and neuroophthalmology. 2009 20 (special issue). WITH. 41-44.
  3. Luk'ianets' OO, Iavors'kyĭ VA. [The action of pilocarpine on the accommodation properties of isolated phasic neurons of the rat hippocampus]. . Fiziol Zh. 1999. 45 N 4 , p. 35-40.
  5. Cheung W., Guo L., Cordeiro M.F. Neuroprotection in glaucoma: drug-based approaches . Optom. Vis. Sci. 2008. 85, N 6. P. 406-416. CrossRef PubMed PubMedCentral
  7. Henne J, Jeserich G Maturation of spiking activity in trout retinal ganglion cells coincides with upregulation of Kv3.1- and BK-related potassium channels . J. Neurosci. Res. 75,N 1. P. 44-54. CrossRef PubMed
  9. Grienkiewicz G., Poenie M., Tsien R.Y. A new genera­tion of Ca2+ indicators with greatly improved fluores­cence properties . J.Biol.Chemistry. 2005. 260, N 6. P. 3440-3450.
  11. Gutman G.A., Chandy K.G., Grissmer S., Lazdunski M., McKinnon D., Pardo L.A., Robertson G.A., Rudy,B., Sanguinetti M.C., Stuhmer W., Wang X. International Union of Pharmacology. LIII. Nomenclature and molecu­lar relationships of voltage-gated potassium channels . Pharmacol. Rev. 2005. 57, N 4. P. 473-508. CrossRef PubMed
  13. Mann M., Haq W., Zabel T., Guenter E., Zrenner E., Ladewig T. Age-dependent changes in the regulation mechanisms for intracellular calcium ions in ganglion cells of the mouse retina . Eur. J. Neurosci. 2005. 22, N 11. P. 2735-2743.
  14. CrossRef PubMed  
  15. O'Brien B.J., Isayama T., Richardson R., Berson, D.M. Intrinsic physiological properties of cat retinal ganglion cells . J.Physiol. 2002. 538, N 3. P. 787-802. CrossRef PubMed PubMedCentral
  17. Rudy B., McBain C.J. Kv3 channels: voltage-gated K+ channels designed for high-frequency repetitive firing. Trends Neurosci. 2001. 24, N 9. P. 517-526. CrossRef  
  18. Wang, G. Y, Robinson, D. W., Chalupa, L. M. Calcium-activated potassium conductances in retinal ganglion cells of the ferret . J.Neurophysiol. 1998. 79, N 1. P. 151-158. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.