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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2011; 57(1): 3-8


Calcium signaling induced by tonic firing in the rat eye retinal ganglion cells

Kuznietsov KI, Maslov VIu, Fedulova SA, Veselovs'kyĭ MS.

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz57.01.003

Abstract

On rat retinal preparation in whole-cell mode retinal ganglion cells (RGC) tonic firing induced calcium signals were recorded and their modulation by Kv3 potassium channels was studied. RGC depolarization with 500 ms current steps caused tonic action potentials (AP) generation and development of calcium signals which amplitude depended quite linearly on mean AP frequency with mean slope 1.4 ± 0.2 s-nmol/l (n = 5). The calcium signals mean tau of decay value was 6.8 ± 1.4 s (n = 5). After application of tetraethylammoniun (500 мM), which in such concentration blocks Kv3.1/Kv3.2 potassium channels, a decrease in firing frequency, an increase of AP width and a decrease of afterhyperpolarization amplitude were observed. In the presence of the blocker the calcium signal amplitude vs mean AP frequency dependency slope mean value increased to 2.1 ± 0.3 s-nmol/l. The calcium signals tan of decay not changed significantly, the mean value was 8.9 ± 1.6 s (n = 5). Thus, voltage-dependent Kv3.1/Kv3.2 potassium channels, which play a key role in tonic firing pattern generation in RGC, also modulate appropriate calcium signals and prevent the cells from significant intracellular calcium increasing and its possible cytotoxic action.

Keywords: retinal ganglion cells, tonic firing, calcium signals,Kv3 potassium channels

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