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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(6): 81-92

Long-term effect of fetal neural tissue late grafting on hippocampus cytoarchitecture after transient global cerebral ischemia in rats

Harashchuk OV, Tsupykov OM, Tsymbaliuk VI

  1. O.O. Bogomoletz National Medical University, Kyiv, Ukraine
  2. O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine


The effect of fetal neural tissue (FNT) grafting on regeneration of hippocampus structure has been investigated in postis­hemic rats. Transient global cerebral ischemia was induced by 20-min four-vessel occlusion in 13.2±2.4-month-old rats. FNT suspension was prepared from hippocampal CA1 area and primordial dentate gyrus of E18-E19 rat fetuses. 30 days after TGI, FNT was stereotactically transplanted into CA1 area of ischemic animals. Linear density of CA1 pyramidal neurons, stratum radiatum width, CA4 and dentate gyrus morphology were studied in hippocampal slices by light microscopy 2, 4 and 7 months after TGI and 1, 3 and 6 months after FNT grafting. It has been shown, that late FNT grafting provides significant and prolonged potentiation of a hippocampal cy-toarchitecture recovery after TGI in rats.

Keywords: fetal neural tissue, forebrain global ischemia, stereotaxic grafting, hippocampus


  1. Koval' I.N., Sarkisov G.T., Gambaryan L.S. Septo-gippokampal'naya sistema i organizatsiya povedeniya. Ierevan: Izd-vo AN ArmSSR, 1986. 127 s.
  3. Skibo G.G., Kovalenko T.M., Osadchenko I.O., Tsupikov O.M., Pivneva T.A. Strukturni zmini v gipokampi pri eksperimental'nii ishemii mozku . Ukr. nevrol. zhurn. 2006. N 1. S 86-92.
  5. Tupikov OM, Pivnev TA, Poddubna AA, Kirik VM, Kuchuk OV, Butenko GM, Skibo GG Migration and differentiation of transplanted fetal neurogenic cells in the brain of ischemic animals . Fiziol zh. . 2009. 55, N 4. p. 41-49.
  7. Bayer S.A. Development of the hippocampal region in the rat. I. Neurogenesis examined with 3H-thymidine autoradiography . J. Comp. Neurol. 1980. 190, N 1. P. 87-114. CrossRef PubMed
  9. Bird C.M., Burgess N. The hippocampus and memory: insights from spatial processing . Nat. Rev. Neurosci. 2008. 9, N 3. P. 182-194. CrossRef PubMed
  11. Bjqrklund A., Lindvall O. Cell replacement therapies for central nervous system disorders . Nat. Neurosci. 2000. 3, N 6. P. 537-544. CrossRef PubMed
  13. Blackstad T.W. Commissural connections of the hip­pocampal region in the rat, with special reference to their mode of termination . J. Comp. Neurol. 1956. 105, N 3. P. 417-537. CrossRef PubMed
  15. Braak H., Braak E. Evolution of neuronal changes in the course of Alzheimer's disease . J. Neural. Transm. Suppl. 1998. 53, N 1. P. 127-140. CrossRef PubMed
  17. Caronna J.J., Finklestein S. Neurological syndromes af­ter cardiac arrest . Stroke. 1978. N 9. P. 517-520. CrossRef PubMed
  19. Englund U., Bjorklund A., Wictorin K., Lindvall O., Kokaia M. Grafted neural stem cells develop into func­tional pyramidal neurons and integrate into host cortical circuitry . Proc. Natl. Acad. Sci. USA. 2002. 99, N 26. P. 17089-17094. CrossRef PubMed PubMedCentral
  21. Field P.M., Seeley P.J., Frotscher M., Raisman G. Se­lective innervation of embryonic hippocampal trans­plants by adult host dentate granule cell axons . Neuroscience. 1991. 41, N 2-3. P. 713-727. CrossRef  
  22. Hodges H., Sowinski P., Fleming P., Kershaw T.R., Sinden J.D., Meldrum B.S., Gray J.A. Contrasting ef­fects of fetal CA1 and CA3 hippocampal grafts on deficits in spatial learning and working memory in­duced by global cerebral ischaemia in rats . Neuro­science. 1996. 72, N 4. P. 959-988. CrossRef  
  23. Liu J., Solway K., Messing R.O., Sharp F.R. Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils . J. Neurosci. 1998. 18, N 19. P. 7768-7778. CrossRef PubMed PubMedCentral
  25. Nitatori T., Sato N., Waguri S., Karasawa Y., Araki H., Shibanai K., Kominami E., Uchiyama Y. Delayed neuronal death in the CA1 pyramidal cell layer of the gerbil hip­pocampus following transient ischemia is apoptosis . J. Neurosci. 1995. 15, N 2. P. 1001-1011. CrossRef PubMed PubMedCentral

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