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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(6): 31-38


Participation of synaptotagmin in release of catecholamines in rat adrenal chromaffin cells

Pochyniuk OV, Zaïka OL, Sadovyĭ OV, Iavors'ka OM, Kostiuk PH, Luk'ianets OO

  1. O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine]
  2. International Center Molecular Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz56.06.031

Abstract

Exocytosis is known to provide such a vital processes as the release of neurotransmitters in synaptic transmission or release of hormones during secretion. The main mechanism of exocytotic process occurs through the specialized protein complex called the SNARE-complex. Due to its activity the fusion of vesicular and plasma membrane occurrs and fusion pore is formed through which a content of vesicles is released outside. It is believed that just synaptotagmins which are Ca2+-dependent proteins, responsible for initiation of the process of Ca2+-dependent exocytosis. Synaptotagmins are located at the membrane of the vesicles and can bind two or three Ca2+ ions. In our research, we studied the role of one of the most common isoform of synaptotagmines - synaptotagmin-1. For this we used an injection of antibodies arised to synaptotagmin-1 (anti-STg-1) into isolated rat adrenal chromaffin cells to depress the function of this protein. Catecholamine secretion was measured by amperometric method. Our results showed that an exclusion of synaptotagmin-1 function in tested cells resulted in significant supression of secretion. These data allow us to conclude that synaptotahmin-1 is a key protein which is needed for Ca2+-dependent exocytosis in chromaffin cells.

Keywords: Chromaffin cells, calcium, synaptotagmin, SNARE-complex, exocytosis, secretion, catecholamines

References

  1. Augustine G.J., Neher E. Calcium requirements for secretion in bovine chromaffin cells . J.Physiol.(Lond). 1992. 450. P. 247-271. CrossRef PubMed PubMedCentral
  2.  
  3. Bhalla A., Chicka M.C., Tucker W.C.,Chapman E.R. Ca(2+)-synaptotagmin directly regulates t-SNARE function during reconstituted membrane fusion . Nat.Struct.Mol.Biol. 2006. 13. P. 323-330. CrossRef PubMed
  4.  
  5. Brose N., Petrenko A.G., Sudhof T.C.Jahn R. Synapto-tagmin: a calcium sensor on the synaptic vesicle surface . Science. 1992. 256. P. 1021-1025. CrossRef PubMed
  6.  
  7. Brunger A.T. Structural insights into the molecular mechanism of Ca(2+)-dependent exocytosis . Curr.Opin.Neurobiol. 2000. 10. P. 293-302. CrossRef  
  8. Burgoyne R.D., Morgan A. Ca2+ and secretory-vesicle dynamics . Trends Neurosci. 1995. 18. P. 191-196. CrossRef  
  9. Dai H., Shen N., Arac D.,Rizo J. A quaternary SNARE-synaptotagmin-Ca2+-phospholipid complex in neuro­transmitter release . J.Mol.Biol. 2007. 367. P. 848-863. CrossRef PubMed PubMedCentral
  10.  
  11. Fasshauer D., Bruns D., Shen B., Jahn R.,Brunger A.T. A structural change occurs upon binding of syntaxin to SNAP-25 . J.Biol.Chem. 1997. 272. P. 4582-4590. CrossRef PubMed
  12.  
  13. Fasshauer D., Otto H., Eliason W.K., Jahn R.,Brunger A.T. Structural changes are associated with soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor complex formation . J.Biol.Chem. 1997. 272. P. 28036-28041. CrossRef PubMed
  14.  
  15. Fasshauer D., Sutton R.B., Brunger A.T., Jahn R. Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs . Proc.Natl.Acad.Sci.USA. 1998. 95. P. 15781-15786. CrossRef PubMed PubMedCentral
  16.  
  17. Fernandez-Chacon R., Konigstorfer A., Gerber S.H., Garcia J., Matos M.F., Stevens C.F., Brose N., Rizo J., Rosenmund C,Sudhof T.C. Synaptotagmin I functions as a calcium regulator of release probability . Nature. 2001. 410. P. 41-49. CrossRef PubMed
  18.  
  19. Geppert M., Goda Y., Hammer R.E., Li C, Rosahl T.W., Stevens C.F.,Sbdhof T.C. Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse . Cell. 1994. 79. P. 717-727. CrossRef  
  20. He B., Guo W. The exocyst complex in polarized exocytosis . Curr.Opin.Cell Biol. 2009. 21. P. 537-542. CrossRef PubMed PubMedCentral
  21.  
  22. Jena B.P. Secretory vesicles transiently dock and fuse at the porosome to discharge contents during cell secretion . Cell Biol.Int. 2010. 34. P. 3-12.
  23.  
  24. Kostyuk P.G., Pochynyuk O.M., Zaika O.L.,Lukya-netz E.A. Roles of nicotinic and muscarinic receptors in calcium signaling and transmitter release . Neurophysiology. 2003. 35. P. 201-207. CrossRef  
  25. Lukyanetz E.A. Role of synaptic proteins in neurotransmitter release-related vesicular trafficking . Neurophysiology. 2008. 40. P. 137-141. CrossRef  
  26. Lukyanetz E.A. Calcium signaling in secretion of catecholamines in chromaffin cells . Fiziol.Zh. 2009. 55. P. 110-111.
  27.  
  28. Lukyanetz E.A., Neher E. Different types of calcium channels and secretion from bovine chromaffin cells . Eur.J.Neurosci. 1999. 11. P. 2865-2873. CrossRef PubMed
  29.  
  30. Marqueze B., Berton F.,Seagar M. Synaptotagmins in membrane traffic: which vesicles do the tagmins tag? . Biochimie. 2000. 82. P. 409-420. CrossRef  
  31. Neher E., Sakaba T. Multiple roles of calcium ions in the regulation of neurotransmitter release . Neuron. 2008. 59. P. 861-872. CrossRef PubMed
  32.  
  33. Otto H., Hanson P.I.,Jahn R. Assembly and disassem­bly of a ternary complex of synaptobrevin, syntaxin, and SNAP.25 in the membrane of synaptic vesicles . Proc.Natl.Acad.Sci.USA. 1997. 94. P. 6197-6201. CrossRef PubMed PubMedCentral
  34.  
  35. Perin M.S., Fried V.A., Mignery G.A., Jahn R.,Sudhof T.C. Phospholipid binding by a synaptic vesicle pro­tein homologous to the regulatory region of protein kinase C . Nature. 1990. 345. P. 260-263. CrossRef PubMed
  36.  
  37. Pochynyuk O.M., Zaika O.L.,Lukyanetz E.A. Role of the mitochondria in generation of acetylcholine-induced calcium transients in rat chromaffin cells . Neuro-fiziologiya. Neurophysiology. 2002. 34. P. 217-219. CrossRef  
  38. Rizo J., Sudhof T.C. C2-domains, structure and func­tion of a universal Ca2+-binding domain . J.Biol.Chem. 1998. 273. P. 15879-15882. CrossRef PubMed
  39.  
  40. Sadovyj A.V., Yavorskaya E.N.,Lukyanetz E.A. Voltammetric detection of quantal release of cathecho-lamines from chromaffin cells . Fiziol.Zh. 2009. 55. P. 140.
  41.  
  42. Schiavo G., Osborne S.L.,Sgouros J.G. Synaptotagmins: more isoforms than functions? . Biochem. Biophys. Res. Commun. 1998. 248. P. 1-8. CrossRef PubMed
  43.  
  44. Shao X., Fernandez I., Sudhof T.C.,Rizo J. Solution structures of the Ca2+-free and Ca2+-bound C2A do­main of synaptotagmin I: does Ca2+ induce a conformational change? . Biochemistry. 1998. 37. P. 16106-16115. CrossRef PubMed
  45.  
  46. Shoji-Kasai Y. , Yoshida A., Sato K., Hoshino T., Ogura A., Kondo S., Fujimoto Y., Kuwahara R., Kato R.,Takahashi M. Neurotransmitter release from synaptotagmin-deficient clonal variants of PC12 cells . Science. 1992. 256. P. 1821-1823. CrossRef PubMed
  47.  
  48. Sudhof T.C., Rizo J. Synaptotagmins: C2-domain pro­teins that regulate membrane traffic . Neuron. 1996. 17. P. 379-388. CrossRef  
  49. Sugita S., Han W., Butz S., Liu X., Fernandez-Chacon R., Lao Y.,Sudhof T.C. Synaptotagmin VII as a plasma membrane Ca(2+) sensor in exocytosis . Neuron. 2001. 30. P. 459-473. CrossRef  
  50. Sutton R.B., Davletov B.A., Berghuis A.M., Sudhof T.C.,Sprang S.R. Structure of the first C2 domain of synaptotagmin I: a novel Ca2+. phospholipid.binding fold . Cell. 1995. 80. P. 929-938. CrossRef  
  51. Zaika O.L., Pochiniuk O.M.,Luk'ianets E.A. Electro­chemical studies of induced secretion of catecholaminesfrom individual vesicles of rat chromaffin cells . Ukr.Biokhim.Zh. 2001. 73. P. 69-72.
  52.  
  53. Zaika O.L., Pochinyuk O.M.,Lukyanetz E.A. Compa­rative characteristics of the secretory responses of chromaffin and pheochromocytoma PC-12 cells to acetylcholine stimulation . Neurophysiology. 2000. 32. P. 174-176. CrossRef  
  54. Zaika O.L., Pochynyuk O.M.,Lukyanetz E.A. Comparative studies of calcium transients induced by acetylcholine in rat chromaffin cells . Neurophy-siology. 2002. 34. P. 261-263. CrossRef  
  55. Zaika O.L., Pochynyuk O.M., Sadovyj A.V., Kostyk P.G.,Lukyanetz E.A. Involvement of the Endoplasmic Reticulum of Chromaffin Cells of the Rat Adrenal Gland in Calcium Signaling . Neurofiziologiya. Neurophysiology. 2009. 41. P. 460-466. CrossRef  

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