Participation of synaptotagmin in release of catecholamines in rat adrenal chromaffin cells
Pochyniuk OV, Zaïka OL, Sadovyĭ OV, Iavors'ka OM, Kostiuk PH, Luk'ianets OO
- O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine]
- International Center Molecular Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz56.06.031
![](/images/cite.png)
Abstract
Exocytosis is known to provide such a vital processes as the release of neurotransmitters in synaptic transmission or release of hormones during secretion. The main mechanism of exocytotic process occurs through the specialized protein complex called the SNARE-complex. Due to its activity the fusion of vesicular and plasma membrane occurrs and fusion pore is formed through which a content of vesicles is released outside. It is believed that just synaptotagmins which are Ca2+-dependent proteins, responsible for initiation of the process of Ca2+-dependent exocytosis. Synaptotagmins are located at the membrane of the vesicles and can bind two or three Ca2+ ions. In our research, we studied the role of one of the most common isoform of synaptotagmines - synaptotagmin-1. For this we used an injection of antibodies arised to synaptotagmin-1 (anti-STg-1) into isolated rat adrenal chromaffin cells to depress the function of this protein. Catecholamine secretion was measured by amperometric method. Our results showed that an exclusion of synaptotagmin-1 function in tested cells resulted in significant supression of secretion. These data allow us to conclude that synaptotahmin-1 is a key protein which is needed for Ca2+-dependent exocytosis in chromaffin cells.
Keywords:
Chromaffin cells, calcium, synaptotagmin, SNARE-complex, exocytosis, secretion, catecholamines
References
- Augustine G.J., Neher E. Calcium requirements for secretion in bovine chromaffin cells . J.Physiol.(Lond). 1992. 450. P. 247-271.
CrossRef
PubMed PubMedCentral
- Bhalla A., Chicka M.C., Tucker W.C.,Chapman E.R. Ca(2+)-synaptotagmin directly regulates t-SNARE function during reconstituted membrane fusion . Nat.Struct.Mol.Biol. 2006. 13. P. 323-330.
CrossRef
PubMed
- Brose N., Petrenko A.G., Sudhof T.C.Jahn R. Synapto-tagmin: a calcium sensor on the synaptic vesicle surface . Science. 1992. 256. P. 1021-1025.
CrossRef
PubMed
- Brunger A.T. Structural insights into the molecular mechanism of Ca(2+)-dependent exocytosis . Curr.Opin.Neurobiol. 2000. 10. P. 293-302.
CrossRef
- Burgoyne R.D., Morgan A. Ca2+ and secretory-vesicle dynamics . Trends Neurosci. 1995. 18. P. 191-196.
CrossRef
- Dai H., Shen N., Arac D.,Rizo J. A quaternary SNARE-synaptotagmin-Ca2+-phospholipid complex in neurotransmitter release . J.Mol.Biol. 2007. 367. P. 848-863.
CrossRef
PubMed PubMedCentral
- Fasshauer D., Bruns D., Shen B., Jahn R.,Brunger A.T. A structural change occurs upon binding of syntaxin to SNAP-25 . J.Biol.Chem. 1997. 272. P. 4582-4590.
CrossRef
PubMed
- Fasshauer D., Otto H., Eliason W.K., Jahn R.,Brunger A.T. Structural changes are associated with soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor complex formation . J.Biol.Chem. 1997. 272. P. 28036-28041.
CrossRef
PubMed
- Fasshauer D., Sutton R.B., Brunger A.T., Jahn R. Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs . Proc.Natl.Acad.Sci.USA. 1998. 95. P. 15781-15786.
CrossRef
PubMed PubMedCentral
- Fernandez-Chacon R., Konigstorfer A., Gerber S.H., Garcia J., Matos M.F., Stevens C.F., Brose N., Rizo J., Rosenmund C,Sudhof T.C. Synaptotagmin I functions as a calcium regulator of release probability . Nature. 2001. 410. P. 41-49.
CrossRef
PubMed
- Geppert M., Goda Y., Hammer R.E., Li C, Rosahl T.W., Stevens C.F.,Sbdhof T.C. Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse . Cell. 1994. 79. P. 717-727.
CrossRef
- He B., Guo W. The exocyst complex in polarized exocytosis . Curr.Opin.Cell Biol. 2009. 21. P. 537-542.
CrossRef
PubMed PubMedCentral
- Jena B.P. Secretory vesicles transiently dock and fuse at the porosome to discharge contents during cell secretion . Cell Biol.Int. 2010. 34. P. 3-12.
- Kostyuk P.G., Pochynyuk O.M., Zaika O.L.,Lukya-netz E.A. Roles of nicotinic and muscarinic receptors in calcium signaling and transmitter release . Neurophysiology. 2003. 35. P. 201-207.
CrossRef
- Lukyanetz E.A. Role of synaptic proteins in neurotransmitter release-related vesicular trafficking . Neurophysiology. 2008. 40. P. 137-141.
CrossRef
- Lukyanetz E.A. Calcium signaling in secretion of catecholamines in chromaffin cells . Fiziol.Zh. 2009. 55. P. 110-111.
- Lukyanetz E.A., Neher E. Different types of calcium channels and secretion from bovine chromaffin cells . Eur.J.Neurosci. 1999. 11. P. 2865-2873.
CrossRef
PubMed
- Marqueze B., Berton F.,Seagar M. Synaptotagmins in membrane traffic: which vesicles do the tagmins tag? . Biochimie. 2000. 82. P. 409-420.
CrossRef
- Neher E., Sakaba T. Multiple roles of calcium ions in the regulation of neurotransmitter release . Neuron. 2008. 59. P. 861-872.
CrossRef
PubMed
- Otto H., Hanson P.I.,Jahn R. Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin, and SNAP.25 in the membrane of synaptic vesicles . Proc.Natl.Acad.Sci.USA. 1997. 94. P. 6197-6201.
CrossRef
PubMed PubMedCentral
- Perin M.S., Fried V.A., Mignery G.A., Jahn R.,Sudhof T.C. Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C . Nature. 1990. 345. P. 260-263.
CrossRef
PubMed
- Pochynyuk O.M., Zaika O.L.,Lukyanetz E.A. Role of the mitochondria in generation of acetylcholine-induced calcium transients in rat chromaffin cells . Neuro-fiziologiya. Neurophysiology. 2002. 34. P. 217-219.
CrossRef
- Rizo J., Sudhof T.C. C2-domains, structure and function of a universal Ca2+-binding domain . J.Biol.Chem. 1998. 273. P. 15879-15882.
CrossRef
PubMed
- Sadovyj A.V., Yavorskaya E.N.,Lukyanetz E.A. Voltammetric detection of quantal release of cathecho-lamines from chromaffin cells . Fiziol.Zh. 2009. 55. P. 140.
- Schiavo G., Osborne S.L.,Sgouros J.G. Synaptotagmins: more isoforms than functions? . Biochem. Biophys. Res. Commun. 1998. 248. P. 1-8.
CrossRef
PubMed
- Shao X., Fernandez I., Sudhof T.C.,Rizo J. Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change? . Biochemistry. 1998. 37. P. 16106-16115.
CrossRef
PubMed
- Shoji-Kasai Y. , Yoshida A., Sato K., Hoshino T., Ogura A., Kondo S., Fujimoto Y., Kuwahara R., Kato R.,Takahashi M. Neurotransmitter release from synaptotagmin-deficient clonal variants of PC12 cells . Science. 1992. 256. P. 1821-1823.
CrossRef
PubMed
- Sudhof T.C., Rizo J. Synaptotagmins: C2-domain proteins that regulate membrane traffic . Neuron. 1996. 17. P. 379-388.
CrossRef
- Sugita S., Han W., Butz S., Liu X., Fernandez-Chacon R., Lao Y.,Sudhof T.C. Synaptotagmin VII as a plasma membrane Ca(2+) sensor in exocytosis . Neuron. 2001. 30. P. 459-473.
CrossRef
- Sutton R.B., Davletov B.A., Berghuis A.M., Sudhof T.C.,Sprang S.R. Structure of the first C2 domain of synaptotagmin I: a novel Ca2+. phospholipid.binding fold . Cell. 1995. 80. P. 929-938.
CrossRef
- Zaika O.L., Pochiniuk O.M.,Luk'ianets E.A. Electrochemical studies of induced secretion of catecholaminesfrom individual vesicles of rat chromaffin cells . Ukr.Biokhim.Zh. 2001. 73. P. 69-72.
- Zaika O.L., Pochinyuk O.M.,Lukyanetz E.A. Comparative characteristics of the secretory responses of chromaffin and pheochromocytoma PC-12 cells to acetylcholine stimulation . Neurophysiology. 2000. 32. P. 174-176.
CrossRef
- Zaika O.L., Pochynyuk O.M.,Lukyanetz E.A. Comparative studies of calcium transients induced by acetylcholine in rat chromaffin cells . Neurophy-siology. 2002. 34. P. 261-263.
CrossRef
- Zaika O.L., Pochynyuk O.M., Sadovyj A.V., Kostyk P.G.,Lukyanetz E.A. Involvement of the Endoplasmic Reticulum of Chromaffin Cells of the Rat Adrenal Gland in Calcium Signaling . Neurofiziologiya. Neurophysiology. 2009. 41. P. 460-466.
CrossRef
|