High frequency firing properties of the rat retinal ganglion cells and role of 4-AP-sensitive potassium current in firing generation were studied using whole cell patch-clamp tech­niques. Potassium channel blocker 4-AP (0,2 mM) reduced depolarization-evoked firing rate by 62% (from 50 ± 5, n = 12 to 19 ± 2 Hz, n = 7). Single action potential parameters were affected: half-width was increased by 133% (from 1,2 ± 0,1, n = 12 to 2,8 ± 0,3 ms, n = 7), decay slope was decreased by 63% (from 102 ± 11, n = 12 to 38 ± 7 mV/ms, n = 7) and afterhyperpolarization amplitude by 83% (from 18 ± 2, n = 12 to 3 ± 2 mV, n = 7). 4-AP application (0,2 mM) reduced po­tassium current amplitude of the retinal neurons. 4-AP-sensi­tive current was obtained by digital subtraction of current that was registered in the presence of the blocker from control current records (1,61 ± 0,22 nA, n = 6). 4-AP-sensitive current was equal to 0,40 ± 0,09 nA (n = 6). Thus, moderate (25%) reducing of potassium current amplitude leads to essential changes in firing properties of retinal neurons. 4-AP-sensitive residual current was similar to Kv3.1/Kv3.2 potassium currents that were described in literature previously. Moreover, we excluded the possibility of 4-AP influence on Kv1 channels by specific blocker a-dendrotoxin (100 nM) application. Such applications significantly affected neither firing frequency nor single action potential parameters. The data obtained suggest that 4-AP-sensitive current plays a key role in high frequency firing generation in the rat retinal ganglion cells. We assume that the 4-AP-sensitive current is mediated by Kv3.1/Kv3.2 conductance.