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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(5): 3-12

Role of nitric oxide in the development of the myocardial contractile reactions in trained animals

Shymans'ka TV, Hoshovs'ka IuV, Sahach VF.

    O.O. Bogomoletz Institute of Physiology, National Academy of Science of Ukraine, Kyiv, Ukraine


Intensive constitutive production of nitric oxide (NO) during physical training improves vasodilatation and heart function. However, it remains unclear how NO takes part in myocardial adaptation to workload, which is accompanied by an increased heart inflow and intracellular calcium content. Using isolated rat heart by Langendorf preparation, we studied myocardial response to gradually increased left ventricular volume (Frank-Starling low) and increasing concentration of Ca2+ in the perfu-sion solution (from 1.7mM to 12,5 mM) in trained and un­trained rats. It was shown that 4 weeks swimming course improved heart function: heart rate was decreased; contractile activity (dP/dt max) and coronary flow were increased by 20% and 33%, respectively. Equal volume stretching of bal­loon in left ventricle provoked greater contraction in trained comparing to untrained hearts, demonstrating extended func­tional reserves after swimming course. Mitochondrial membrane potential was significantly increased in hearts of trained rats. Furthermore, training prevented fast increase of the end dias-tolic pressure during calcium upload. Mitochondrial factor release due to opening of mitochondrial permeability transi­tion pore (MPTP) in trained hearts was detected at higherconcentrations of calcium that reveals extended calcium capacity of mitochondria and lesser sensitivity of MPTP to its induc­tor – calcium. Blockade of NO synthesis with L-NAME ap­plication of (10-4 M for 15 min) abolished reaction of trained heart during Frank-Starling and calcium upload. Thus, heart adaptation to physical training and extension of functional reserves in heart are provided by endogenous NO production.

Keywords: nitric oxide, Frank-Starling low, physical training,calcium upload, mitochondrial permeability transition,membrane potential


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