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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(4): 86-95

Laminar distribution of the active spinal cord neurons during the feeding-related stereotyped movements in the rat

Vlasenko OV, Dovhan' OV, Maĭs'kyĭ VO, Piliavs'kyĭ OI, Maznychenko AV

  1. M.I. Pirogov National Medical University, Vinnitsa, Ukraine
  2. O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine


The comparative study of expression of early c-fos-gene (marker of neuronal activation) and NADPH-diaphorase reactivity (NADPH-dr) was performed in the cervical spinal cord of rats in the control (intact) animal, in the state of starva­tion and after realization of long-lasting (repeated 4 to 12 times per minute for 30 min) motivated stereotyped food-procuring forelimb movements. In comparison with control rats; in the starving rats or rats showed forelimb movement to reach-to-grasp the food, the number of Fos-immunoreactive (Fos-ir) cells in the dorsal and ventral horns of a 40-um-thick slice was significantly greater (P < 0.05). The number of Fos-ir neurons in the starving state clearly exceeded that in the most layers after realization of movements. Increase of Fos immunoreactivity in the superficial (2i, 3) and deeper (4, 5) layers of the dorsal horn was initiated, evidently, by signals from peripheral and supraspinal structures. We also found labelled cells within layers 6-8, and 9 demonstrating the activ­ity of interneurons and motoneurons directly involved into generation of operant forelimb movements. According to our data, high density of NADPH-dr/NO-generating neurons in the C6/C7 segments are observed in the substance gelatinosa (layer 2i) and layers 7 and 10. NADPH-dr cells and Fos-ir neurons were intermixed within the layers but did not demon­strate double-labelling. It is possible to suggest that NADPH-dr/NO-generating cells of the spinal cord did not operate under realization of the studied operant reflexes, which did not in­clude nociceptive component.

Keywords: operant reflex, expression c-fos, NADPH-diaphorase reactivity, spinal cord, rat.


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