Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(4): 86-95


Laminar distribution of the active spinal cord neurons during the feeding-related stereotyped movements in the rat

Vlasenko OV, Dovhan' OV, Maĭs'kyĭ VO, Piliavs'kyĭ OI, Maznychenko AV

  1. M.I. Pirogov National Medical University, Vinnitsa, Ukraine
  2. O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz56.04.086

Abstract

The comparative study of expression of early c-fos-gene (marker of neuronal activation) and NADPH-diaphorase reactivity (NADPH-dr) was performed in the cervical spinal cord of rats in the control (intact) animal, in the state of starva­tion and after realization of long-lasting (repeated 4 to 12 times per minute for 30 min) motivated stereotyped food-procuring forelimb movements. In comparison with control rats; in the starving rats or rats showed forelimb movement to reach-to-grasp the food, the number of Fos-immunoreactive (Fos-ir) cells in the dorsal and ventral horns of a 40-um-thick slice was significantly greater (P < 0.05). The number of Fos-ir neurons in the starving state clearly exceeded that in the most layers after realization of movements. Increase of Fos immunoreactivity in the superficial (2i, 3) and deeper (4, 5) layers of the dorsal horn was initiated, evidently, by signals from peripheral and supraspinal structures. We also found labelled cells within layers 6-8, and 9 demonstrating the activ­ity of interneurons and motoneurons directly involved into generation of operant forelimb movements. According to our data, high density of NADPH-dr/NO-generating neurons in the C6/C7 segments are observed in the substance gelatinosa (layer 2i) and layers 7 and 10. NADPH-dr cells and Fos-ir neurons were intermixed within the layers but did not demon­strate double-labelling. It is possible to suggest that NADPH-dr/NO-generating cells of the spinal cord did not operate under realization of the studied operant reflexes, which did not in­clude nociceptive component.

Keywords: operant reflex, expression c-fos, NADPH-diaphorase reactivity, spinal cord, rat.

References

  1. Vlasenko O.V., Dovgan' O.V., Shlyavs'kii O.I., Mais'kii V.O., Maznichenko A.V. Zmini ekspresii c-fos ta NADFN-diaforaznoi aktivnosti v riznih yadrah gipotalamusa pid chas vidstoronennya vid izhi abo realizatsii operantnih izhodobuvnih ruhiv u shchuriv . Neirofiziologiya . Neurophysiology. 2009. 41, N 2. S. 173-182. CrossRef  
  2. Vlasenko OV, Maysky VO, Maznichenko AV, Shlyavsky OI, Moroz VM Investigation of the expression of c-fos and NADPH-diaphorase activity in the spinal cord and brain in the development of neck muscle fatigue in rats . Fiziol zh. . 2006. 52, N 6. p. 3-14.
  3.  
  4. Vlasenko O.V., Pilyavskii A.I., Maiskii V.A., Maznichenko A.V. Fos-immunoreaktivnost' i NADFN-d-reaktivnost' v kore bol'shih polu­sharii kris, realizuyushchih motivirovannie stereo­tipnie dvizheniyah perednei konechnost'yu . Neiro­fiziologiya . Neurophysiology. 2008. 40, N 4. S. 351-361. CrossRef  
  5. Dovgan' O.V., Vlasenko O.V., Mais'kii V.O., Shlyavs'kii O.I., Maznichenko A.V. Topografiya Fos-imunoreaktivnih ta NADFN-d-reaktivnih neironiv u limbichnih strukturah osnovi peredn'ogo mozku ta gipotalamusi pri realizatsii motivovanih stereo­tipnih ruhiv u shchuriv . Neirofiziologiya . Neuro­physiology. 2009. 41, N 1. S. 19-27.
  6.  
  7. Maysky VA, Pilyavsky OI, Maznichenko AV, Datsenko VV, Pavlyuchenko VB, Kostyukov O.1., Moibenko OO Central neural circuits of the Betzold Jarish reflex in rats . Fiziol zh. . 2005. 51, N 2. p. 24-38.
  8.  
  9. Abbadie C., Skinner K., Mitrovic I., Basbaum A.I. Neurons in the dorsal column white matter of the spinal cord: complex neuropil in an unexpected location . Proc. Natl. Acad. Sci. USA. 1999. 96. P. 260-265. CrossRef PubMed PubMedCentral
  10.  
  11. Adkins D.L., Boychuk J., Remple M.S., Kleim J.A. Motor training induces experience-specific patterns of plasticity across motor cortex and spinal cord . J. Appl. Physiol. 2006. 101. P. 1776-1782. CrossRef PubMed
  12.  
  13. Ahn S.N., Guu J.J., Tobin A.J., Edgerton V.R., Tillakaratne N.J. Use of c-fos to identify activity-de­pendent spinal neurons after stepping in intact adult rats . Spinal Cord. 2006. 44. P. 547-559. CrossRef PubMed PubMedCentral
  14.  
  15. Bannatyne B.A., Edgley S.A., Hammar I., Jankowska E., Maxwell D.J. Differential projections of excitatory and inhibitory dorsal horn interneurons relaying information from group II muscle afferents in the cat spinal cord . J. Neurosci. 2006. 26. P. 2871-2880. CrossRef PubMed PubMedCentral
  16.  
  17. Brown A.G. Organization of the Spinal Cord. The Anatomy and Physiology of Identified Neurons. New York: Springer, 1981. 238 p.
  18.  
  19. Dai X., Noga B.R., Douglas J.R., Jordan L.M. Localization of spinal neurons activated during locomotion using the c-fos immunohistochemical method . J. Neurophysiol. 2005. 93. P. 3442-3452. CrossRef PubMed
  20.  
  21. Gamboa-Esteves F.O., Tavares I., Almeida A., Batten T.F., McWilliam P.N., Lima D. Projection sites of su­perficial and deep spinal dorsal horn cells in the nucleus tractus solitarii of the rat . Brain Res. 2001. 921. P. 195-205. CrossRef  
  22. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase tech­niques: a comparison between ABC and unlabelled anti­body (PAP) procedures . J. Histochem. Cytochem. 1981. 29. P. 577-580. CrossRef PubMed
  23.  
  24. Hunt S.P., Pini A., Evan G. Induction of c-fos-like pro­tein in spinal cord neurons following sensory stimula­tion . Nature. 1987. 328. P. 632-634. CrossRef PubMed
  25.  
  26. Jasmin L., Gogas K.R., Ahlgren S.C., Levine J.D., Basbaum A.I. Walking evokes a distinctive pattern of Fos-like immunoreactovity in the caudal brainstem and spinal cord of the rat . Neurosci. 1994. 58. P. 275-286. CrossRef  
  27. Kalueff A.V., Maisky V.A., Pilyavskii A.I., Makarchuk N.E. Persistent c-fos expression and NADPH-d reactivity in the medulla and the lumbar spinal cord in rat with short-term peripheral anosmia . Neurosci. Lett. 2001. 301. P. 131-134. CrossRef  
  28. Kleim J.A., Lussnig E., Schwarz E.R., Comery T.A., Greenough W.T. Synaptogenesis and Fos expression in the motor cortex of the adult rat after motor skill learning . Neurosci. 1996. 16. P. 4529-4535. CrossRef PubMed PubMedCentral
  29.  
  30. Luth H.J., Hedlich A., Hilbig H., Winkelmann E., Mayer B. Morphological analyses of NADPH-diaphorase. ni-tric oxide synthase positive structures in human visual cortex . J. Neurocytol. 1994. 23. P. 770-782. CrossRef PubMed
  31.  
  32. Marsala J., Lukacova N., Cizkova D., Lukac I., Kucharova K., Marsala M. Premotor nitric oxide synthase immuno-reactive pathway connecting lumbar segments with the ventral motor nucleus of the cervical enlargement in the dog . J. Chem. Neuroanat. 2004. 27. P. 43-54. 20.Maznychenko A.V., Pilyavskii A.I., Kostyukov A.I., Lyskov E., Vlasenko O.V., Maisky V.A. Coupling of c-fos expression in the spinal cord and amygdala induced by dorsal neck muscles fatigue . Histochem. Cell Biol. 2007. 128. P. 85-90. CrossRef PubMed
  33.  
  34. Monfils M.H., Plautz E.J., Kleim J.A. In search of the motor engram: motor map plasticity as a mechanism for encoding motor experience . Neuroscientist. 2005. 11. P. 471-483. CrossRef PubMed
  35.  
  36. Morgan J.I., Cohen D.R., Hempstead J.L., Curran T. Mapping patterns of c-fos expression in the central nervous system after seizure . Science. 1987. 237. P. 192-197. CrossRef PubMed
  37.  
  38. Paxinos G., Watson C. The rat brain in stereotaxic coordinates. San Diego: Acad. Press, 1997.
  39.  
  40. Pilyavskii A.I., Maisky V.A., Kalezic I., Ljubisavljevic M., Kostyukov A.I., Windhorst U., Johansson H. c-fos expression and NADPH-diaphorase reactivity in spinal neurons after fatiguing stimulation of hindlimb muscles in the rat . Brain Res. 2001. 923. P. 91-102. CrossRef  
  41. Vincent S.R., Kimura H. Histochemical mapping of nitric oxide synthase in the rat brain . Neuroscience. 1992. 46. P. 755-784. CrossRef  
  42. Wang Y., Pillai S., Wolpaw J.R., Chen X.Y. Motor learning changes GABAergic terminals on spinal mo­toneurons in normal rats . Eur. J. Neurosci. 2006. 23. P. 141-150. CrossRef PubMed
  43.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.