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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(4): 26-36


Characteristics of sensory neurotransmission in co-culture of neurons from the dorsal root ganglion and dorsal horn spinal cord in rats

Shypshyna MS, Veselovs'kyĭ MS

    O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz56.04.026

Abstract

We examined properties of chemical neurotransmission at the level of primary afferent inputs into spinal cord with the new easy-to-use in vitro model of contiguously-cultivated dissoci­ated both the dorsal root ganglion neurons (DRG) and the dorsal horn spinal cord neurons (DHSC) from newborn rats. The results of our studies showed the presence of excitatory and inhibitory DRG neurons synapses on the cells of DHSC. The excitatory afferent signaling in such synapses was medi­ated by presynaptic release of glutamate and a following acti­vation of both NMDA- and non-NMDA-receptor subclasses. In these cases the activation of non-NMDA-receptors makes a main contribution to realization of excitatory postsynaptic effects. Either glycine- or GABA-ergic DRG neurons were involved in transmission of inhibitory signals to the DHSC neurons. However, in vast majority of examined neuronal pairs the inhibitory synaptic transmission was mediated by pr-esynaptic release of glycine. As distinct from the previous similar methods, an in vitro model of co-culture of both the DRG and the DHSC neurons proposed here allows to use comprehensively modern technical approaches for examina­tion of the transmission of somatosensory information from the periphery to the CNS. The described model could be ac­ceptable for detailed investigation of specific properties of primary afferent synapses.

Keywords: dorsal root ganglion, dorsal horn spinal cord, coculture, synaptic transmission, NMDA- receptors, nonNMDA-receptors, GABA, glycine, evoked postsynaptic currents.

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