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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(4): 19-25


INCREASED EXPRESSION OF VOLTAGEDEPENDENT ANION CHANNEL AND ADENINE NUCLEOTIDE TRANSLOCASE AND THE SENSITIVITY OF CA2+ -INDUCED MITOCHONDRIAL PERMEABILITY TRANSITION OPENING PORE IN OLD RAT HEART

S.V. Chorna, V.E. Dosenko, N.A. Strutynska, G.L. Vavilova, V.F. Sagach

    O.O. Bogomoletz Institute of Physiology, National Academyof Science of Ukraine, Kyiv


Abstract

We investigated mRNA and protein expression of voltage-dependent anion channel (VDAC), mRNA adenine nucleotide translocase (ANT) as well as the sensitivity of the mitochondrial permeability transition pore opening (MPTP) to Ca2+ in the adult and old rat heart. It was shown that in the old rats hearts VDAC mRNA expression increased by 1,7 (p<0,05) times and mRNA ANT expression increased by 1,8 (p<0,05) times in comparison with adult animals. The Western Blot analysis showed that the level of VDAC protein expression in the old rat hearts also significantly increased compared with adult animals. In the hearts of old rats, the sensitivity of MPTP opening to calcium (10-7 - 10-4 mol/l) determined by mitochon­dria swelling, increased two-fold (p<0,05). Therefore, an in­creased VDAC and ANT expression, as the main structural functional components of the MPTP, and an increased sensi­tivity of MPTP opening to Ca2+ caused an increase in the permeability of mitochondrial membranes in aging. Each of these factors may contribute to alterations in mitochondrial barrier properties and lead to mitochondrial dysfunction.

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