Warning: include(phj2-admin/kernel/config.php): failed to open stream: No such file or directory in /zstorage3/vhosts/fz.kiev.ua/httpdocs/index.php on line 45

Warning: include(): Failed opening 'phj2-admin/kernel/config.php' for inclusion (include_path='.:/usr/local/share/pear') in /zstorage3/vhosts/fz.kiev.ua/httpdocs/index.php on line 45

Warning: Cannot modify header information - headers already sent by (output started at /zstorage3/vhosts/fz.kiev.ua/httpdocs/index.php:45) in /zstorage3/vhosts/fz.kiev.ua/httpdocs/kernel/content.php on line 3

Warning: array_unique() expects parameter 1 to be array, null given in /zstorage3/vhosts/fz.kiev.ua/httpdocs/kernel/content.php on line 77

Warning: Invalid argument supplied for foreach() in /zstorage3/vhosts/fz.kiev.ua/httpdocs/kernel/content.php on line 86
Fiziologichnyi Zhurnal - <a id="lj1_ret_bk_to_jl0_href_id" href="?list0=12">Fiziol. Zh. 2010;</a> <a id="lj1_ret_bk_to_jl0_href_id" href="?list1=58">56(1):</a> 118-126
Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(1): 118-126


CALCIUM-DEPENDENT REGULATION OF DEPRESSION OF INHIBITORY SYNAPTIC TRANSMISSION BY BLOCKER OF N-TYPE CA2+ CHANNELS IN CULTURED HIPPOCAMPAL NEURONS

O.P. Mizerna, S.A. Fedulova, N.S. Veselovsky.

    O.O.Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv


Abstract

Multiple types of high-voltage-activated Ca2+ channels trigger neurotransmitter release at the mammalian central synapse. Now it is clear that N-type Ca2+ channels contribute to synap-tic transmission at many of CNS synapses. However, it is not known whether presynaptic N-type Ca2+ channels contribute to calcium-dependent paired pulse depression (PPD) medi­ated by GABA release at inhibitory synapses of cultured hippocampal neurons. We studied the sensitivity of GABAergic depression to selective N-type high-voltage-activated Ca2+ channels blocker omega-conotoxin- GVIA (w-CgTx) under dif­ferent calcium concentrations. Evoked inhibitory postsynap-tic currents were studied using patch-clamp technique in whole-cell configuration in postsynaptic neuron and local exteracellular paired - pulse stimulation of single presynaptic axon by rectangular pulse with 0.4 ms duration, the interpulse interval in pair was 150 ms. The present data show that the relationship between eIPSC and extracellular Ca2+ concentration are highly nonlinear. The fraction of N-type Ca2+ channel in calcium-dependent PPD was estimated in presence of w-CgTx (200 nM). The application of w-CgTx did not change nonlinear relationship. It was shown that PPD is qualitatively affected by the extracellular Ca2+ concentration. Lowering external Ca2+ concentration reduced the depression powerfully in presence of w-CgTx than in absence. In addition we found difference between the degree of cooperativity for transmitter release in the presence and absence of the N-type Ca2+ channel blocker. So, at inhibitory synapses in the hippocampus more than one Ca2+ ions act in a cooperative manner to cause release of GABA. After w-CgTx application the degree of cooperativity nH was increased. These results confirm that N-type Ca2+ channels are highly involved in calcium-dependent paired pulse depres­sion at inhibitory synapse in cultured hippocampal neurons.

Keywords: N-type calcium channels, omega-conotoxin GVIA,GABAergic synaptic transmission, paired pulse depression,cooperative action of calcium.

References

  1. Мізерна О.П., Федулова С.А., Веселовський М.С. Вплив тапсигаргіну на гальмівну синаптичну передачу в культурі нейронів гіпокампа щура// Нейрофизио­логия/Neurophysiology. - 2007. - 39, №4/5. - P. 374-376.
  2. Debanne D., Gu&ineau N.C., Gflhwiler B.H., Thompson S.M. Paired-pulse facilitation and depression at unitary syn­apses in rat hippocampus: quantal fluctuation affects subse­quent release// J. Physiol. - 1996. - 491. - P. 163-176.
  3. Dodge Jr. F.A., Rahamimoff R. Co-operative action of calcium ions in transmitter release at the neuromuscular junction//J. Physiol (Lond). - 1967. - 193. - P. 419-432.
  4. Fagg G.E.,. Foster A.C. Amino acid neurotransmitters and their pathways in the mammalian central nervous system// Neuroscience - 1983. - V 9. - P. 701-719.
  5. Forsythe I. D., Tsujimoto T., Barnes-Davies M., Cuttle M.F., Takahashi T. Inactivation of presynaptic calcium current contributes to synaptic depression at a fast central synapse//Neuron. - 1998. - 20. - P. 797-807.
  6. Horne A.L., Kemp J.A. The effect of щ-conotoxin GVIA on synaptic transmission within the nucleus accumbens and hippocampus of the rat in vitro// Brit. J. Pharmacol. - 1991.- 103. - P.1733 - 1739.
  7. Jensen K., Lambert J.D., Jensen M.S. Activity-dependent depression of GABAergic IPSCs in cultured hippocampal neurons// J. Neurophysiol. - 1999. - 82. - P. 42-49.
  8. Katz B., Miledi R. The effect of calcium on acetylcho­line release from motor nerve terminals. // Proc. R. Soc. Lond B Biol. Sci. - 1965. - 161. - P. 496-503.
  9. 9. Katz B., Miledi R. The role of calcium in neuromuscular facilitation// J. Physiol. - 1968. - 195. - P.481-492.
  10. 10. Luebke J.l., Dunlap K., Turner T.J. Multiple calcium channel types control glutamatergic synaptic transmission in the hippocampus// Neuron. -1993. - 11. - P.895– 902.
  11. Macdonald R. L. and Olsen R. W. GABAA receptor channels// Annu. Rev. Neurosci. - 1994. - 17. - P. 569-602.
  12. Ohno-Shosaku T., Hirata K., Sawada S., Yamamoto C. Contributions of multiple calcium channel types to GABAergic transmission in rat cultured hippocampal neurons// Neurosci. Lett. - 1994. - 181. - P. 145-148.
  13. Reid C.A., Bekkers J.M., Clements J.D. N-and P/Q-Type Ca2+ channels mediate transmitter release with a similar cooperativety at rat hippocampal autaps// J. Neuroscience. - 1998. - 18. - P. 2849-2855.
  14. Reid C.A., Clements J.D., Bekkers J.M. Nonuniform distribution of Ca2+ channel subtypes on presynaptic terminals of excitatory synapses in hippocampal cultures// J. Neurosci. - 1997. - 15. - P. 2738-2745.
  15. Stanley E. F. Decline in calcium cooperativity as the basis of facilitation at the squid giant synapse// J. Neu­rosci. - 1986. - 6(3). - P. 782 - 789.
  16. Takahashi T., Momiyama A. Different types of calcium channels mediate central synaptic transmission// Na­ture. -1993. - 366. - P.156 -158.
  17. Veselovsky N.S., Engert F, Lux H.D. Fast local superfusion technique//Pflugers Arch. -1996. - 432. - P. 351-354.
  18. Weiss J.N. The Hill equation revisited: uses and misuses//FASEB J. - 1997. - 11. - P. 835-841.
  19. 19. Wu L.G., Borst J.G. The reduced release probability of releasable vesicles during recovery from short-term synaptic depression//Neuron. - 1999. - 23. - P. 821-832.
  20. 20. Xiaoming Wang, S.N. Treistman, J.R. Lemos. Two types of high-threshold calcium currents inhibited by co-conotoxin in nerve terminals of rat neurohypophysis// J. Physiol. - 1992. - 445. - P. 181-199.
  21. Zucker R.S., Regehr W.G. Short-term synaptic plas­ticity// Annu. Rev. Physiol. - 2002. - 64. - P. 355-405.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.