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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(1): 109-117


The role of nitric oxide in theregulation of colonic water andelectrolyte transport in normalcolon and during acetic acidexperimental colitis in rats

T.M. Savka, G.M Tolstanova, Ja.M. Savitsky,V.I. Kimakovich.

    Danylo Galytskyi Lviv National University;Taras Shevchenko Kyiv National University


Abstract

Despite numerous studies about the role of NO in the regulation of intestinal water and ion transport, the data about colonic fluid movement are controversial and scanty. The aim of the present study was to investigate the contribution of NO in the basal net water and electrolytes (Na+, K+, Cl-) transport in normal colon and during experimental ulcerative colitis in rats. The net water and electrolytes movements were evaluated by isolated colonic loop perfusion technique in vivo on anaesthetized male Wistar rats. Experimental colitis was modeled by rectal injection of 1 ml 4% acetic acid. In the present study, precursor of NO L-arginine (500 mg/kg, i.p.) had no effect on net water,K+ and Cl- transport, but significantly decreased (P<0.05) Na+ ab­sorption. Injection of inhibitor NOS L-NAME (10mg/kg, i.v.) did not affect the net water and СI- movement, but induced elevation of the net Na+ absorption and a slight amelioration of K+ secretion with a significant difference P<0.01. We found profound downregulation of net water, Na+ absorption and K+ secretion which were associated with colonic mucosal ulceration at 3rd day after acetic acid introduction. Despite restoration of colonic mucosae at 7th day, Na+ absorption was not restored completely, moreover K+ secretion was still significantly de­creased (P<0.01). L-arginine administration decreased the net Na+ absorption and didn’t affect the net water, K+ and Cl-movement at 1st and 3rd days after acetic acid enema. L-NAME slightly increased the net Na+ absorption (P<0.01) only at 1st day and had any effect at 3rd day. Conclusion: NO might have slight pro-secretory influence on colonic fluid transport in vivo via regulation of Na+ and K+ transport.

Keywords: nitric oxide, ion transport, water transport, colon,ulcerative colitis

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