Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(1): 72-78


Effect of nitrogen oxides and hydrogen peroxide to transport Ca2 + in the reticulum of the permeabilized rat uterine myocytes

Iu.V. Danylovych.

    O.V. Palladin Biochemical Institute NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz56.01.072

Abstract

Investigations were conducted on a model of digitonin-permeabilized myocytes from uterus of pig with the use of 45Ca2+. It is set that in presence 10 mM of sodium azide which reliably represses the energy-dependent accumulation of Ca2+ in mitochondria, sodium nitropruside (10 uM - 0,1 mM), nitrite-anione (0,1 uM - 10 uM) and hydrogen peroxide (0,1 uM - 10 uM) stimulated the energy-dependent including of Ca2+ in sarcoplasmic reticulum. At probed processes of the passive freeing of Ca2+ from this pool which was preliminary accumulated in a ATP-dependent process, it is discovered that sodium nitropruside (10 uM), nitrite-anione (10 nM - 0,1 mM) and hydrogen peroxide (10 nM) result in the decline of the passive release of Ca2+, while 0,1 mM H2O2 resulted in an opposite effect. The findings allow to suggest that the probed chemicals can reduce the concentration of Ca2+ in myoplasm, and hence, the contractile activity, operating at the sarcoplas-mic reticulum level.

Keywords: nitrogen oxide, hydrogen peroxide, calcium, sar-coplasmic reticulum, utera.

References

  1. Babich LG, Shlykov SG, Borisova LA Energy-dependent transport of Ca2 + in intracellular smooth muscle structures . Biochemistry. 1994. 69, no. 8. P. 1218-1229.
  2.  
  3. Danilovich GV, Danilovich YV Influence of nitric oxides and hydrogen peroxide on Ca2 +, Mg2 + ATP-azne and Mg2 + ATP-azine activity of myometrial sarcolemma . Ukr. biochem. . 2007. 79, N 2. P. 30-37.
  4.  
  5. Danilovich Yu.V. The influence of steroid hormones and oxytocin on the formation of NO and H O in the endometrium . Ukr. biochem. 2004. 76, N 1. P. 828-96.
  6.  
  7. Danilovich Yu.V. Characteristics of passive Ca2 + exit from the sarcoplasmic reticulum of rat myometrial cells . Fiziol zh.  - 2007. 53, N 1. pp. 55-61.
  8.  
  9. Adachi T., Matsui R., Weisbrod R.M. Reduced sarco. endoplasmic reticulum Ca2+ uptake activity can account for the reduced response to NO, but not sodium nitro-prusside, in hypercholesterolemic rabbit aorta . Cir­culation. 2001. 104, N 9. P. 1040-1045. CrossRef PubMed
  10.  
  11. Adachi T., Weisbrod R.M., Pimentel D.R. S-Glutathiolation by peroxynitrite activates SERCA dur­ing arterial relaxation by nitric oxide . Nat. Med. 2004. 10, N 11. P. 1200-1207. CrossRef PubMed
  12.  
  13. Babich L.G., Burdyga Th.V., Shlykov S.G. Evidence for the intracellular nonmitochondrial calcium store in uterine smooth muscle cells .Ukr.biohim.zhurn. 1997. 69,N 2. p. 19-29.
  14.  
  15. Barata H., Thompson M., Zielinska W. The role of cyclic-ADP-ribose-signaling pathway in oxytocin-in-duced Ca2+-transients in human myometrium cells . Endocrin. 2004. 145, N 2. P. 881-889. CrossRef PubMed
  16.  
  17. Brotto M.A., Nosek T.M. Hydrogen peroxide disrupts Ca2+ release from the sarcoplasmic reticulum of rat skeletal muscle fibers . J. Appl. Physiol. 1996. 81, N 2. P. 731-737. CrossRef PubMed
  18.  
  19. Buxton I.L. Regulation of uterine function: a biochemicalconundrum in the regulation of smooth muscle relaxation . Mol. Pharmacol. 2004. 65, N 5. P. 1051-1059. CrossRef PubMed
  20.  
  21. Chung D., Caruso R.L. Potential role for oxidative stress in 2,2'-dichlorobiphenyl-induced inhibition of uterine contractions but not myometrial gap junctions . Toxicol. Sci. 2006. 93, N 1. P. 172-179. CrossRef PubMed
  22.  
  23. Cohen R.A., Weisbrod R.M., Gericke M. Mechanism of nitric oxide-induced vasodilation: refilling of intrac­ellular stores by sarcoplasmic reticulum Ca ATPase and inhibition of store-operated Ca2+ influx . Circulat. Res. 1999. 84, N 2. P. 210-219. CrossRef PubMed
  24.  
  25. Fujimoto S., Asano T., Sakai M. Mechanisms of hydrogen peroxide-induced relaxation in rabbit mesenteric small artery . Eur. J. Pharmacol. 2001. 412, N 3. P. 291-300. CrossRef  
  26. Gen W., Tani M., Takeshita J. Mechanisms of Ca2+ overload induced by extracellular H O in quiescent isolated rat car-diomyocytes . Basic. Res. Car2diol. 2001. 96, N 6. P. 623-629. CrossRef PubMed
  27.  
  28. Goldhaber J.I., Liu E. Excitation-contraction coupling in single guinea-pig ventricular myocytes exposed to hydro­gen peroxide . J. Physiol. 1994. 477. N 1. P. 135-147. CrossRef PubMed PubMedCentral
  29.  
  30. Hoffman P., Stanke-Labesque F., Fanchin R. Effects of L-arginine and sodium nitroprusside on the spontane­ous contractility of human non-pregnant uterus . Human Reproduction. 2003. 18, N 1. P. 148-151. CrossRef PubMed
  31.  
  32. Kannan M.S., Prakash Y.S., Johnson D.E. Nitric oxide inhibits calcium release from sarcoplasmic reticulum of porcine tracheal smooth muscle cells . Amer. J. Physiol. 1997. 272, N 1. P.L. 1-7. CrossRef PubMed
  33.  
  34. Kargacin M.E., Kargacin G.J. Direct measurment of Ca2+ uptake and release by the sarcoplasmic reticulum of saponin permeabilized isolated smooth muscle cells . J. Gen. Physiol. 1995. 106. P. 467-484. CrossRef PubMed PubMedCentral
  35.  
  36. Kobayashi T., Taguchi K., Takenouchi Y. Insulin-induced impairment via peroxynitrite production of endothelium-dependent relaxation and sarco. endoplasmic reticulum Ca ATPase function in aortas from diabetic rats . Free Radic. Biol. Med. 2007. 43, N 3. P. 431-440. CrossRef PubMed
  37.  
  38. Kuenzli K.A., Bradley M.E., Buxton I.L. Cyclic GMP-inde-pendent effects of nitric oxide on guinea-pig uterine contractil­ity . Brit. J. Pharmacol. 1996. 119, N 4. P. 737-743. CrossRef PubMed PubMedCentral
  39.  
  40. Laporte R., Hui A., Laher J. Pharmacological modula­tion of sarcoplasmic reticulum in smooth muscle . Pharmacol. Rev. 2004. 46, N 4. P. 439-513. CrossRef PubMed
  41.  
  42. MacFarlane N.G., Miller D.J., Smith G.L. Effects of oxidants on the sarcoplasmic reticulum of saponin treated rat ventricular trabeculae . Cardiovascular. Res. 1994. 28, N 11. P. 1647-1652. CrossRef PubMed
  43.  
  44. Matoba T., Shimokawa H. Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in animals and humans . J. Pharmacol. Sci. 2003. 92. P. 1-6. CrossRef PubMed
  45.  
  46. Matthew A., Shmygol A., Wray S. Ca2+ entry, efflux and release in smoth muscle . Biol. Res. 2004. 37, N 4. P. 617-624. CrossRef PubMed
  47.  
  48. Mollard P., Mironneau J., Amedee T. Electrophysi­ological characterization of single pregnant rat myometrial cells in short-term primary culture . Amer. J. Physiol. 1986. 19, N 1. P.C. 47-54. CrossRef PubMed
  49.  
  50. Norman J.E., Cameron I.T. Nitric oxide in the human uterus . Rev. Reprod. 1996. 1. P. 61-68. CrossRef PubMed
  51.  
  52. Oba T., Kurono C, Nakajima R. H O activates ryano-dine receptor but has little effect on recovery of releasable Ca2+ content after fatigue . J. Appl. Physiol. 2002. 93, N 6. P. 1999-2008. CrossRef PubMed
  53.  
  54. Okawa T., Vedernikov Y.P., Saade G.R. Effect of nitric oxide on contractions of uterine and cervical tissues from pregnant rats . Gynecol. Endocrinol. 2004. 18, N 4. P. 186-193. CrossRef PubMed
  55.  
  56. Pucovsky V., Gordienko D.V., Bolton T.B. Effect of nitric oxide donors and noradrenaline on Ca2+ release sites and global intracellular Ca2+ in myocytes from guinea-pig small mesenteric arteries . J. Physiol. 2002. 539, N 1. P. 25-39. CrossRef PubMed PubMedCentral
  57.  
  58. Ross R., Klebanoff S.J. The smooth muscle cell. In vivo synthesis of connective tissue proteins . J. Cell Biol 1971. 50. P. 159-171. CrossRef PubMed PubMedCentral
  59.  
  60. Sanchez S., Fernandez-Belda F., Solef F. Functional effect of hydrogen peroxide on the sarcoplasmic reticulum membrane: uncoupling and irreversible inhibition of the Ca2+-ATPase protein . Arch. Biochem. and Biophys. 2004. 431, N 2. P. 245-251. CrossRef PubMed
  61.  
  62. Sanders K.M. Mechanisms of calcium handling in smooth muscles . J. Appl. Physiol. 2001. 91. P. 1438-1449. CrossRef PubMed
  63.  
  64. Shen J.Z., Zheng X.F., Wei E.Q. Evidence against inhi­bition of sarcoplasmic reticulum Ca2+-pump as mecha­nism of H O induced contraction of rat aorta . Acta Pharmacol. Sin. 2001. 22, N 6. P. 498-504.
  65.  
  66. Sladek S.M., Magness R.R., Conrad K.P Nitric oxide and preg-nancy . Amer. J. Physiol. 1997. 272, N 41. P.R. 441-463. CrossRef PubMed
  67.  
  68. Suzuki Y.J., Ford G.D. Inhibition of Ca2+-ATPase of vascular smooth muscle sarcoplasmic reticulum by reactive oxygen intermediates . Ibid. 1991. 261, N 2. PH. 568-574. CrossRef PubMed
  69.  
  70. Thomas S.R., Chen K., Keaney J.F. Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway . J. Biol. Chem. 2002. 277, N 8. P. 6017-6024. CrossRef PubMed
  71.  
  72. Wada S., Okabe E. Susceptibility of caffeine- and Ins (1,4,5)P3-induced contractions to oxidants in permeabilized vascular smooth muscle . Eur. J. Pharmacol. 1997. 320, N 1. P. 51-59. CrossRef  
  73. Warren AY., Matharoo-Ball B., Shaw R.W Hydrogen peroxide and superoxide anion modulate pregnant human myometrial contractility . Reproduction. 2005. 130. P. 539-544. CrossRef PubMed
  74.  
  75. Wray S., Kupittayanant S., Shmygol A. The physi­ological basis of uterine contractility: a short review . . Exp. Physiol. 2001. 86, N 2. P. 239-246. CrossRef PubMed
  76.  
  77. Ying J., Tong X., Pimentel D.R. Cystein-674 of the sarco. endoplasmic reticulum calcium ATPase is required for the inhibition of cell migration by nitric oxide . Arterioscler. Thromb. Vasc. Biol. 2007. 27, N 4. P. 783-790. CrossRef PubMed
  78.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.