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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2010; 56(1): 72-78


The action of nitrogen oxides andhydrogen peroxide on ca[sup]2+[/sup] transportin sarcoplasmic reticulumof permeabilizated myocytesof utera

Iu.V. Danylovych.

    O.V. Palladin Biochemical Institute NAS of Ukraine, Kyiv


Abstract

Investigations were conducted on a model of digitonin-permeabilized myocytes from uterus of pig with the use of 45Ca2+. It is set that in presence 10 mM of sodium azide which reliably represses the energy-dependent accumulation of Ca2+ in mitochondria, sodium nitropruside (10 uM - 0,1 mM), nitrite-anione (0,1 uM - 10 uM) and hydrogen peroxide (0,1 uM - 10 uM) stimulated the energy-dependent including of Ca2+ in sarcoplasmic reticulum. At probed processes of the passive freeing of Ca2+ from this pool which was preliminary accumulated in a ATP-dependent process, it is discovered that sodium nitropruside (10 uM), nitrite-anione (10 nM - 0,1 mM) and hydrogen peroxide (10 nM) result in the decline of the passive release of Ca2+, while 0,1 mM H2O2 resulted in an opposite effect. The findings allow to suggest that the probed chemicals can reduce the concentration of Ca2+ in myoplasm, and hence, the contractile activity, operating at the sarcoplas-mic reticulum level.

Keywords: nitrogen oxide, hydrogen peroxide, calcium, sar-coplasmic reticulum, utera.

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