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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2001; 47(1): 3-8


Spontaneous transient outward currentsin smooth muscle cells of the rat tail artery

V. L. Reznikov, M. F Shuba

    A. A. Bogomoletz Institute of Physiology,National Academy of Sciences of Ukraine, Kiev


Abstract

V. L. Reznikov, M. F Shuba SPONTANEOUS TRANSIENT OUTWARD CURRENTS IN SMOOTH MUSCLE CELLS OF THE RAT TAIL ARTERY Spontaneous transient outward currents (STOCs) were studied in the rat tail artery smooth muscle cells using standard patch-clamp recording techniques in the wholecell configuration. STOCs evoked by membrane depolarization from .30 to 20 mV varied in size from 50 to 1000 pA, their amplitude increased with membrane depolarization. These currents were inhibited by 1 mM TEA+. Both the frequency and the transferred charge (Q) were decreased in the absence of the extracellular Ca2+ or in the presence of the selective blocker of voltage-gated L-type Ca2+ channels nifedipine. Application of caffeine at 1 mM increased both Q and the frequency of STOCs generation. These results indicate that STOCs are carried by large conductance Ca2+-dependent K+ channels and Ca2+ influx plays an important role in their activation. A. A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev

References

  1. СПИСОК ЛІТЕРАТУРИ
  2. Бурый В.А., Гордиенко Д.В., Шуба М.Ф. Характеристика калиевой проводимости мембраны изолированной гладкомышечной клетки мезентериальной артерии //Биол.мембраны. — 1992. — 9, №6. — С. 595-601.
  3. Ганиткевич В.Я., Шуба М.Ф. Спонтанные выходящие токи в мембране изолированной гладкомышечной клетки коронарной артерии // Там же. — 1988. — 5, №12.— С. 1312-1320. Спонтанні вихідні струми. 8 ISSN 0201-8489 O?c?ie. ?o?i., 2001, O. 47, ? 1
  4. Anwer K., Oberti C., Perez G.J. et al. Calcium-activated K+ channels as modulators of human myometrial contractile activity //Amer. J.Physiol. ? 1993. ? 265, (Cell Physiol. 34). ? P. C976-C985.
  5. Bolton T.B., Imaizumi Y. Spontaneous transient outward currents in smooth muscle cells // Cell Calcium. ? 1996. ? 20, ?2. ? P. 141-152.
  6. Benham C.D. and Bolton T.B. Spontaneous transient outward currents in single visceral and vascular smooth muscle cells of rabbit //J.Physiol. ? 1986. ? 381. ? P. 385-406.
  7. Bychkov R, Gollasch M., Ried Ch. et al. Regulation of spontaneous transient outward potassium currents in human coronary arteries //Circulation. ? 1997. ? 95. P. 503-510.
  8. Bonev A.D., Jaggar J.H., Rubart M., Nelson M.T. Activators of protein kinase C decrease Ca2+ spark frequency in smooth muscle cells from cerebral arteries //Amer. J.Physiol. ? 1997. ? 273. ? P. C2090-C2095.
  9. Ganitkevich V.Ya., Isenberg G. Efficacy of peak Ca2+ currents (Ica) as trigger ofdarcoplasmic reticulum Ca2+ release in myocytes from the guinea-pig coronary artery // J.Physiol. ? 1995. ? 484, ?2. ? P. 287-306.
  10. 9. Hamil O.P., Marty A., Neher E. et al. Improved patch-clamp techniques for highresolution current recording from cells and cell-free membrane patches //Pflugers Arch. ? 1981. ? 391. ? P. 85-100.
  11. 10. Hatakeyama N., Mukhopadhyay D., Goyal R.K., Akbarali H.I. Tyrosine kinasedependent modulation of calcium entry in rabbit colonic muscularis mucosae //Amer. J.Physiol. ? 1996. ? 270. ? P. C1780-C1789.
  12. Hecker M. Endothelium-derived hyperpolarizing factor ? fact or fiction? //NIPS 2000. ? 15. ? P. 1-5.
  13. Kang T.M., So. I., Kim K.W. Caffeine- and histamine-induced oscillations K(Ca) current in single smooth muscle cells of rabbit cerebral artery //Pflugers Arch. 1995. ? 431. ? P. 91-100.
  14. Komori S., Bolton T.B. Actions of guanine nucleotides and cyclic nucleotides on calcium stores in single patch-clamped smooth muscle cells from rabbit portal vein //Brit.J.Pharmacol. ? 1989. ? 97. ? P. 973-982.
  15. Milesi V., Aiello E.A., Rebolledo A. et al. Role of Ca2+-activated K+-current in the maintenance of resting membrane potential of isolated, human, saphenous vein smoothmuscle cells //Pflugers Arch. ? 1999. ? 437. ? P. 455-461.
  16. Nelson M.T., Cheng H., Rubart M. et al. Relaxation of arterial smooth muscle by calcium sparks //Science. ? 1995. ? 270. ? P.633-637.
  17. Nelson M.T., Patlak J.B., Worley J.F., Standen N.B. Calcium chasnnels, potassium channels and voltage dependence of arterial smooth muscle cell tone // Amer. J. Physiol. ? 1990. ? 259, (Cell Physiol 28). ?P. C3-C18.
  18. Ohya Y., Kiramura K., Kuriyama H. Cellular calcium regulates outward currents in rabbit intestinal smooth muscle cell // Ibid. ? 1987. ? 252. ? P. C401-C410.
  19. Standen N.B., Quayle J.M. K+ channel modulation in arterial smooth muscle // Acta. Physiol. Scand. ? 1998. ? 164. ? P. 549-557.
  20. 19. Vandier C., Delpech M., Bonnet P. Spontaneous transient outward currents and delayed rectifier K+ current: effects of hypoxia //Amer. J. Physiol. ? 1998. ? 275, ?1. ?P.L145-L154.
  21. 20. Zholos A.V., Baidan L.V., Shuba M.F. Properties of the late transient outward currentin isolated intestinal smooth muscle cells of the guinea-pig //J.Physiol. ?1991. 443. ? P. 555-574.
  22. Zholos A.V., Baidan L.V., Shuba M.F. The inhibitory action of caffeine on calcium currents in isolated intestinal smooth muscle cells //Pflugers Arch. ? 1991. ? 419, ?3-4. ? P. 267-73.

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