Influence of supplementary vitamins and minerals on lipidperoxidation and redox state in heart, kidney and liver from rats exposed to fluoride
J. Khalili, H. F. Biloklytska
National Medical Academy of Postgraduate Education, Kyiv
Abstract
The effect of fluoride (F) and supplementary vitamins and minerals on lipid peroxidation (LPO) and redox state (RS) in heart, kidney and liver of 40 (4 groups of 10) male Wistar rats were studied. One group of rats was left untreated as control, group 1 was received 5 mg/l NaF in their drinking water, group 2 was received 5 mg/l NaF in their drinking water plus vitamins (A, C, and D) in their diet, and group 3 was received 5 mg/l NaF in their drinking water plus vitamins (A, C, and D) and minerals (Mg-, Mn-, Zn-sulfate, and Na-citrate) in their diet. In comparison with the group 2, 3 and controls, elevated malondialdehyde (MDA) content in the group 1 indicated an increase in LPO product. In addition, unsteady ratios of oxidized to reduced nicotinamide adenine dinucleotide (NAD+/NADH) reflected significant alterations in the RS status. These results demonstrate that the combination of vitamins and minerals supplementation proved to restore MDA content and establish steady RS status that has not been previously reported.
References
- Krishnamachari K.A. Skeletal fluorosis in humans: a review of recent progress in the understanding of the disease // Prog. Food Nutr. Sci. - 1986. - 10. - P. 279-314.
- Langseth L. Oxidants and antioxidants: some basic concepts. In: Oxidants, antioxidants and disease prevention. - Belgium: International Life Science Institute Press, 1996. - P. 1-4.
- Chinoy N.J., Patel D. Influences of fluoride on biological free radicals in ovary of mice and its reversal // Environ. Sci. - 1998. - 6. - P. 171-184.
- Carlson J.R., Suttie J.W. Pentose phosphate pathway enzymes and glucose oxidation in fluoride-fed rats // Amer. J. Physiol. - 1966. - 210. - P. 79-83.
- Vani M.L., Reddy K.P. Effects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice // Fluoride. - 2000. - 33. - P. 17-26.
- Park S., Ajtai K., Burghardt P. Inhibition of myosin ATPase by metal fluoride complexes // Biochem. Biophys. Acta. - 1999. - 1430. - P. 127-140.
- Khalili J., Biloklytska H.F., Pogrebnyak H.V. Acid-base status and fructose diphosphatase activity in rats exposed to fluoride and induced periodontitis // Fiziol. Zh. - 2009. - 55, №3. - P. 118-124.
- Pollak N., Dirlle C, Ziegler M. The power to reduce: pyridine nucleotides - small molecules with a multitude of functions // Biochem J. - 2007. - 402. -P. 205-218.
- 9. Palacios C, Joshipura K., Willett W. Nutrition and health: guidelines for dental practitioners // Oral Dis. -2009. - 15. - P. 369-381.
- 10. Abdollahi M., Ranjbar A., Shadnia S. et al. Pesticides and oxidative stress: a review // Med. Sci. Monit. -2004. - 10. - P. RA141-147.
- Stalnaya I.D., Garishvili T.G. The method of deter-mines the malondialdehyde with the aid of thiobar-bituric acid. In: Modern Methods in Biochemistry / Ed. V. N. Orekhovich. – Moscow: Meditsina, 1977. – P. 66–68.
- Putilina F.E. Determination of oxidized and reduced nicotinamide adenine dinucleotide in tissue. In: Methods of Biochemistry Investigations / Ed. M. I. Prohorova. – Leningrad: Leni Uni Press, 1982. – P. 161–166.
- Pendrys D.G. Fluoride ingestion and oral health // J. Nutrition. – 2001. – 17. – P. 979–980.
- Shanthakumari D., Srinivasalu S., Subramanian S. Effect of fluoride intoxication on the levels of intestinal antioxidants studied in rats // Methods Find Exp. Clin. Pharmacol. – 2007. – 29. – P. 93–99.
- Wang A.G., Xia T., Chu Q.L. et al. Effects of fluoride on lipid peroxidation, DNA damage and apoptosis in human embryo hepatocytes // Biomed. Environ. Sci. – 2004. – 17. – P. 217–222.
- Ghosh D., Das Sarkar S., Maiti R. et al. Testicular toxicity in sodium fluoride treated rats: association with oxidative stress // Reprod. Toxicol. – 2002. – 16. – P. 385–390.
- Zhan X.A., Xu Z.R., Li J.X. et al. Effects of fluorosis on lipid peroxidation and antioxidant systems in young pigs // Fluoride. – 2005. – 38. – P. 157–161.
- Kumar T., Mohan E.M., Ramesh N. et al. Toxicity of combination of fluoride and monocrotophos 36% SL to wistar rats. // J. Environ. Biol. – 1998. – 19. – P. 305–311.
- 19. Chlubek D., Grucka–Mamczar E., Birkner E. et al. Activity of pancreatic antioxidative enzymes and malondialdehyde concentrations in rats with hyperg-lycemia caused by fluoride intoxication. // J. Trace. Elem. Med. Biol. – 2003. – 17. – P. 57–60.
- 20. Rzeuski R., Chlubek D., Machoy Z. Interactions between fluoride and biological free radical reactions // Fluoride. – 1998. – 31. – P. 43–45.
- Suh J.H., Shigeno E.T., Morrow J.D. et al. Oxidative stress in the aging rat heart is reversed by dietary supplementation with (R)-a-lipoic acid // FASEB J. – 2001. – 15, N 3. – P. 700–706.
- Williamson J.R., Chang K., Frangos M. et al. Hyperglycemic pseudohypoxia and diabetic complications // Diabetes. - 1993. - 42. - P. 801-813.
- Diaz P.I., Zilm P.S., Wasinger V. et al. Studies on NADH oxidase and alkyl hydroperoxide reductase produced by Porphyromonas gingivalis // Oral Microbiol. Immunol. - 2004. - 19. - P. 137-143.
- Krebs H.A. Pyridine nucleotides and rate control. In: Rate Control of Biological Processes/ Ed. D. D. Davies. -Cambridge, England: Cambridge University Press, 1973. - P. 241-281.
- Williamson D.H., Lund P., Krebs H.A. Redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver // Biochem. J. - 1967. -103. - P. 514-527.
- Greenbaum A.L., Gumaa K.A., McLean P. The distribution of hepatic metabolites and the control of carbohydrate metabolism in animals of different dietary and hormonal states // Arch. Biochem. Biophys. -1971. - 143. - P. 617-663.
- Emsley J., Jones D.J., Miller J.M. et al. An Unexpectedly Strong Hydrogen Bond: ab initio Calculations and Spectroscopic Studies of Amide-Fluoride Systems // J. Am. Chem. Soc. - 1981. - 103. - P. 24-28.
- Wadhwani T.K. Prevention and mitigation of fluorosis (Endemic) Part II // Ind. Med. Sci. - 1954. - 36. -P. 64-69.
- 29. Hennon D.K., Stookey G.K., Muhler J.C. Fluoride Excretion with Sodium Fluoride-Vitamin Tablets // J. Dent. Res. - 1968. - 47. - P. 710-716.
- 30. Petrushko O.E., Zhedik A.N. Results of using Vitafluor for dental caries prevention in 1st graders in Brest // Stomatologiia (Mosk). - 1985. - 64. - P. 84-85 (Article in Russian).
- Margolis F.J., Nagler R.C., Holkeboer P.E. Short-term fluoride excretion in young children // Am. J. Dis. Child. -1967. - 113. - P. 673-676.
- Reddy D.R., Rao C.R., Nayak R.K. et al. Estimations of trace elements elements in drinking water supplies of Podili, Darsi and Kanigiri areas of Andhra Pradesh. In: Proceedings of the workshop on medical geology, IGCP-454. - Nagpur, 2004. - P. 384-96.
- Reddy D.R. Some observations of fluoride toxicity // NIMHANS J. - 1985. - 3. - P. 79-86.
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