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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(6): 63-68


HYPOXIC PULMONARY HYPERTENSION: THEROLE OF PHOSPHATIDYLCHOLINE-SPECIFICHYPOXIC PULMONARY HYPERTENSION: THE ROLE OF PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE C

Z.A.gorenko, L.S.karbovska, Sepideh Parchami Ghazaee Mehdi, I.A.lukyanenko,S.P.veselsky

    ДУ “Ін-т фармакології та токсикології АМНУкраїни”; Київ


Abstract

The aim of the study was to investigate the role of phosphati-dylcholine-specific phospholipase C (PC-PLC) in the deve­lopment of hypoxic pulmonary hypertension. Induction of hypoxic hypoxia in rats resulted in a biphasic increase in sys­tolic right ventricular pressure (RVP). The first phase was transient (5.4 ± 0,9 min), while the second phase was sus­tained and persisted during the whole time course of hypoxia (120 min). An inhibition of cNOS with L-NAME (25 mg/kg; i/v) did not cause any change in the nature of the hypoxic reaction, despite the RVP values were significantly higher in normoxia and hypoxia. These results demonstrate that NO synthase is not substantially involved in the hypoxic pulmo­nary vasoconstriction. Injection of PKC inhibitor chelerythrine (0.5 mg/kg; i/v) prevented the development of the first phase of the RVP increase and reduced the second phase by 40%. An inhibition of PC-PLC with D609 (5 mg/kg; i/v) completely abolished pulmonary hypertension. The results obtained sug­gest that PC-PLC possibly plays a key role in the develop­ment and maintenance of hypoxic pulmonary hypertension.

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