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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(6): 3-11


Effects ofgenipin on no synthesis and ishemia/reperfusion-induced oxidaive stress in old rathearts

Y.V. Goshovska, Y.P. Korkach, T.V. Shimanskaya, A.V. Kotsuruba, V.F. Sagach

    Ін-т фізіології ім. О.О. Богомольця НАН України, Київ


Abstract

Genipin is aglycone of geniposide, one of the active compounds of Gardenia gasminoides Ellis. The gardenia fruit extract has been used in traditional Chinese medicine to relieve the symp­toms of type 2 diabetes that is accompanied with extensive oxidative stress and endothelial dysfunction of NO produc­tion. Besides, genipin was shown to inhibit UCP-depended proton leak through the inner mitochondrial membrane that leads to increased membrane potential and ATP production. We studied the effects of genipin at ischemia/reperfusion-in-duced oxidative stress and activity of NOS isozymes using Langendorf perfused old rat heart model. Ischemia/reperfusion is well-known oxidative agent, and showed significant increas­ing of superoxide radical, hydrogen peroxide and hydroxyl radical. Genipin application in doze 10-5 mol/L for 15 min before prolonged ischemia exerted powerful antiradical and antilipoperoxidative effects. Heart ischemia/reperfusion was supported with peroxynitrite generation and nitrozative stress. We demonstrated the inhibitory property of genipin on iNOS expression that possibly occurs via protein kinase A inhibition and stabilization of I-kB-NF-kB complex. Genipin stimu­lated cNOS activity seemingly activating PI3K/Akt signaling pathway. Although, post-ischemic recovery of cardiodynamic parameters of old rat hearts were depressed due to “switching off the NO production by inducible NOS which is important in early period of reperfusion. Thus, we conclude that genipin is powerfull antioxidant and posses insulin-like activity due to its property of managing the NO production at intracellular signal transduction cascade level.

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