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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 91-99


Genderdifferences in the cold sensitivity: role of hormonal regulation of trpm8 channel

A. P. Kondratskyi, K. O. Kondratska, R. Skryma, N. Prevarskaya, IA. M. Shuba

    Міжнар. центр молекуляр. фізіології НАН України, Київ;Ін-т фізіології ім. О.О. Богомольця НАН України, Київ;Лільськ. ун-т наук та технологій, Франція


Abstract

We have studied the gender differences in the perception of cutaneous cold stimuli in the innocuous temperature range (5-30oC) in mice and rats. In the behavioral tests using two variable temperature plates technique female subjects displayed lower threshold for the sensation of cooling temperatures in the range of 15-25oC compared to males. Patch-clamp experi­ments carried out on dorsal root ganglion (DRG) neurons from male and female rats maintained in the short-term cultures in the presence of testosterone or 17b-estradiol, respectively, have revealed gender- and hormone-related differences in the electrophysiological properties of cold/menthol-sensitive TRPM8 channel: average density of menthol-activated ITRPM8 current density in females’ DRG neurons was higher compared to males’, and the steady-state voltage-dependent activation curve of TRPM8 in females was shifted towards hyper-polarized potentials compared to males. These distinctive TRPM8 properties vanished upon withdrawal of testostero­ne and 17b-estradiol from the culture mediums. We conclude that the observed differences in the behavioural sensitivity to innocuous cold and in functional properties of TRPM8 cold receptor are due to differential regulation of TRPM8 by sex steroid hormones, testosterone and/or 17b-estradiol.

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