Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 41-49


Migration and differentiation of fetal neural progenitor cells in brainof ischemic animals

O.M.tsupykov, T.A.pivneva, A.O. Poddubna, V.M.kyryk, O.V. Kuchuk, G.M. Butenko,G.G.skibo

    Ін-т фізіології ім. О.О. Богомольця НАН України, КиївДУ «Ін-т генетич. та регенерат. медицини АМН України»


Abstract

The migration, integration and differentiation of fetal neural progenitor cells (NPCs) in the ischemic brain have been stud­ied. In our study the ischemic insult in FVB line mice was produced by occlusion of both carotid arteries during 20 min. A day after occlusion NPCs from GFP-transgenic 12.5 dpc embryos were suboccipitally transplanted to the ischemic brain. The migration and differentiation of GFP-positive NPCs in the recipient tissue were observed in different time points after their transplantation by immunohistochemical approaches using confocal scanning microscopy. It has been shown that GFP–positive NPCs survived, migrated and differentiated to the mature neurons and glial cells in CA1 area of hippocampus of ischemic animals.

References

  1. Остроумов Е.Н., Крашенинников М.Е., Гуреев СВ. и др. Визуализация распределения в миокарде аутологичных стволовых клеток костного мозга при их интракоронарном и интрамиокардиальном введении // Вест. трансплантология и искусств. органов. - 2004. -№1. -С. 26-29.
  2. Cho T., Bae J.H., Choi H.B. et al. Human neural stem cells: electrophysiological properties of voltage-gated ion channels // Neuroreport. - 2002 -13, № 43. -P. 1447-1452.
  3. Chu K., Kim M., Jeong S.W. et al. Human neural stem cells can migrate, differentiate, and integrate after in­travenous transplantation in adult rats with transient forebrain ischemia // Neurosci. Lett. - 2003. - 343, №2. - 129-133.
  4. Coulombel L. Identification of hematopoietic stem/ progenitor cells: strength and drawbacks of functional assays // Oncogene. - 2004. - 23, № 43. - P. 7210-7222.
  5. Euglund U., Bjorklund A., Wictorin K. et al. Grafted neural stem cells develop into functional pyramidal neurons and integrate into host cortical circuitry // Proc. Natl. Acad. Sci. U S A. - 2002. - 99, № 26. - P. 17089-17094.
  6. Gage F.H. Mammalian neural stem cells // Science. -2000. - 287, №5457. - P. 1433-1438.7. Jeong S.W., Chu K., Jung K.H. et al. Human neural stem cell transplantation promotes functional recovery in rats with experimental intracerebral hemorrhage // Stroke. - 2003. - 34, № 9. - P. 2258-2263.
  7. Kokaia Z., Lindvall O. Neurogenesis after ischaemic brain insults // Curr. Opin. Neurobiol. - 2003. - 13, №1. - P. 127-132.
  8. 9. Kuhn H.G., Dickinson-Anson H. and Gage F.H. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation // J. Neurosci. - 1996. - 16, № 6. - P. 2027-2033.
  9. 10. Lipton P. Ischemic cell death in brain neurons // Physiol. Rev. - 1999. - 79, №4. - P.1431-1568.
  10. Lois C, Alvarez-Buylla A. Proliferating subventricular zone cells in the adult mammalian forebrain can differ­entiate into neurons and glia // Proc. Natl. Acad. Sci. USA. - 1993. - 90, №5. - P. 2074-2077.
  11. Mattson M.P., Liu D. Energetics and oxidative stress in synaptic plasticity and neurodegenerative disorders // Neuromol. Med. - 2002. - 2, № 2. - P. 215-231.
  12. Mokudai T., Ayoub I.A., Sakakibara Y. et al. Delayed treatment with nicotinamide (vitamin B3) improves neurological outcome and reduces infarct volume after transient focal cerebral ischemia in Wistar rats // Stroke. -2000. - 31, №7. - P. 1679-1685.
  13. Morrison S.J., Wandycz A.M., Hemmati H.D. et al. Identification of a lineage of multipotent hematopoi­etic progenitors // Development. - 1997. - 124, №10. -P. 1929-39.
  14. Nakatomi H., Kuriu T., Okabe S. et al. Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of endogenous neural progeni­tors // Cell. - 2002. - 110, №4. - P. 429-441.
  15. Neuroglia / Edited by H.Kettenmann, B.R. Ransom. -2nd ed. - NY: Oxford University Press, 2005. - 601 p.
  16. O’Neill M.J., Clemens J.A. Rodent Models of Global Cerebral Ischemia // Current Protocols in Neuroscience. -2000. - Supp.12, unit 9.5. - P. 1-25.
  17. Parent J.M., Vexler Z.S., Gong C. et al. Rat forebrain neurogenesis and striatal neuron replacement after fo­cal stroke // Ann. Neurol. - 2002. - 52, №6. - P. 802-813.
  18. 19. Savitz S.I., Rosenbaum D.M., Dinsmore J.H. et al. Cell transplantation for stroke // Ann. Neurol. - 2002. -52, №3. - P. 266-275.
  19. 20. Song H.J., Stevens C.F., Gage F.H. Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons // Nat. Neurosci.- 2002. -5, №5. - P. 438-445.
  20. Toda H., Takahashi J., Mizoguchi A. et al. Neurons generated from adult rat hippocampal stem cells form functional glutamatergic and GABAergic synapses in vitro // Exp. Neurol. - 2000. - 165, №1. - P. 66-76.
  21. Van Praag H., Schinder A.F., Christie B.R. et al. Func­tional neurogenesis in the adult hippocampus // Na­ture. - 2002. - 415, № 6875. - P. 1030-1034.
  22. Vicario-Abejon C, Collin C, Tsoulfas P., McKay R.D.G. Hippocampal stem cells differentiate into ex­citatory and inhibitory neurons // Eur. J. Neurosci. -2000. - 12, №2. - P. 677-688.
  23. Winkelmann E. R., Charcansky A., Faccioni-Heuser M. C. et al. An ultrastructural analysis of cellular death in the CA1 field in the rat hippocampus after transient forebrain ischemia followed by 2, 4 and 10 days of reperfusion // Anat. Embryol. (Berl). - 2006. - 211, № 5. - P. 423-434.
  24. Wong A.M., Hodgesb H., Horsburgh K. Neural stem cell grafts reduce the extent of neuronal damage in a mouse model of global ischaemia // Brain Res. - 2005. -1063, №2. - P. 140-150.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.