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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 24-31


PARTICIPATION OF CA2+-ATPASE IN CALCIUM HOMEOSTASIS OF CEREBELLAR NEURONS IN CRUCIAN

I.A. Lukyanets, P.G. Kostyuk, E.A. Lukyanetz

    O.O.Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz55.04.024

Abstract

In our experiments the participation of endoplasmic reticulum Ca2+-ATPase (SERCA) was studied as a separate intracellular participant of calcium homeostasis in neurons of cerebellum from hypoxia-tolerant fish species – crucian Carassius gibelio. The SERCA’s blockers cyclopiazonic acid and tapsigargin were used. Intracellular Ca2+ concentration ([Ca2+]i) was measured by Ca2+-sensitive dye Fura-2AM and microfluorescent method for measuring free Ca2+ concentration. We established that cerebellar neurons of crucian have a well expressed system to clean the cytoplasm from Ca2+ overflow that is presented by SERCA-pump of endoplasmic reticulum. The switching off a SERCA-pump by means of its selective blockers results in increase of the Ca2+-transient amplitude and Ca2+ entrance in the cell approximately by 20% -100 % in comparison to con­trol depending on duration of depolarization. It is assumed, that SERCA-pump of crucian neurons like in mammals sub­stantially affects temporary and amplitude characteristics of calcium signals in neurons and plays a considerable role in cleaning of cytoplasm from Ca2+ during functional activity of the cell.

Keywords: Calcium ATPase - SERCA- pump, hypoxia-tolerant species, European carp, calcium, neurons, cerebellum

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