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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 17-23

THE ROLE OF N-TYPE HIGH-VOLTAGEACTIVATED CA2+ CHANNELS TO GABAERGIC SHORT-TERM SYNAPTIC PLASTICITY IN CULTURED HIPPOCAMPAL NEURONS

O.P. Mizerna, S.A. Fedulova, N.S. Veselovsky

    O.O.Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine



Abstract

Now it is clear that N-type Ca2+ channels contribute to synap­tic transmission at many of CNS synapses. However, it is not known whether presynaptic N-type Ca2+ channels contribute to short - term synaptic plasticity (STP) mediated by GABA release at inhibitory synapses of cultured hippocampal neurons. We studied the sensitivity of GABAergic paired pulse depres­sion (PPD) as a common form of STP to selective N-type high-voltage-activated Ca2+ channels blocker omega-conotoxin (CgTx). Evoked inhibitory postsynaptic currents (eIPSCs) were studied using patch-clamp technique in whole-cell configuration in postsynaptic neuron and local exteracellular paired pulse stimulation of single presynaptic axon by rectangular pulse with 0.4 ms duration, the interpulse interval in pair was 150 ms. CgTx (200 nM; 1 uM) in a dose-depen­dent manner irreversibly reduced the amplitude of paired eIPSCs by 25 - 49% and decreased PPD by 11 - 22 % compared with control. These results confirm that N-type Ca2+ channels are highly involved in inhibitory synaptic transmission and short-term synaptic plasticity in cultured hippocampal neurons.

Keywords: N-type calcium channels, ω-conotoxin GVIA, GABAergic synaptic transmission, paired pulse depression

Full text: (PDF, in Ukrainian)

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