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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 17-23


O.P. Mizerna, S.A. Fedulova, N.S. Veselovsky

    O.O.Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine


Now it is clear that N-type Ca2+ channels contribute to synap­tic transmission at many of CNS synapses. However, it is not known whether presynaptic N-type Ca2+ channels contribute to short - term synaptic plasticity (STP) mediated by GABA release at inhibitory synapses of cultured hippocampal neurons. We studied the sensitivity of GABAergic paired pulse depres­sion (PPD) as a common form of STP to selective N-type high-voltage-activated Ca2+ channels blocker omega-conotoxin (CgTx). Evoked inhibitory postsynaptic currents (eIPSCs) were studied using patch-clamp technique in whole-cell configuration in postsynaptic neuron and local exteracellular paired pulse stimulation of single presynaptic axon by rectangular pulse with 0.4 ms duration, the interpulse interval in pair was 150 ms. CgTx (200 nM; 1 uM) in a dose-depen­dent manner irreversibly reduced the amplitude of paired eIPSCs by 25 - 49% and decreased PPD by 11 - 22 % compared with control. These results confirm that N-type Ca2+ channels are highly involved in inhibitory synaptic transmission and short-term synaptic plasticity in cultured hippocampal neurons.

Keywords: N-type calcium channels, ω-conotoxin GVIA, GABAergic synaptic transmission, paired pulse depression


  1. Veselovskii N.S., Fedulova S.A., Kostyuk P.G. Biofizika odinochnogo sinapsa. K.: Nauk. dumka, 2004. 118 p.
  2. Brody D.L., Yue D.T. Release-independent short-term synaptic depression in cultured hippocampal neurons. J. Neurosci. 2000. 20. P. 2480-2494.
  3. Dietrich D., Kirschstein T., Kukley M. et al. Func­tional specialization of presynaptic Cav2.3 Ca2+ channels. Neuron. 2003. 39. P. 483-496.
  4. Fagg G.E., Foster A.C. Amino acid neurotransmitters and their pathways in the mammalian central nervous system. Neuroscience. 1983. 9. P. 701-719.
  5. Forsythe I. D., Tsujimoto T., Barnes-Davies M. et al. Inactivation of presynaptic calcium current contributes to synaptic depression at a fast central synapse. Neuron. 1998. 20. P. 797-807.
  6. Gasparini S., Kasyanov A.M., Pietrobon D. et al. Pre­synaptic R-type calcium channels contribute to fast excitatory synaptic transmission in the rat hippocampus. J. Neurosci. 2001. 21. P. 8715-8721.
  7. Horne A.L., Kemp J.A. The effect of w conotoxin GVIA on synaptic transmission within the nucleus accumbens and hippocampus of the rat in vitro. Brit. J. Pharmacol. 1991. 103. P.1733-1739.
  8. Jensen K., Jensen M.S., Lambert J.D. Role of presyn­aptic L-type Ca2+ channels in GABAergic synaptic transmission in cultured hippocampal neurons. J. Neurophysiol. 1999. 81. P. 1225-1230.
  9. 9. Jensen K., Lambert J.D., Jensen M.S. Activity-depen­dent depression of GABAergic IPSCs in cultured hippocampal neurons. J. Neurophysiol. 1999. 82. P. 42-49.
  10. 10. Katz B., Miledi R. The role of calcium in neuromuscular facilitation. J. Physiol. 1968. 195. P.481-492.
  11. Luebke J.l., Dunlap K., Turner T.J. Multiple calcium channel types control glutamatergic synaptic trans­mission in the hippocampus. Ibid. 1993. 11. P.895-902.
  12. Neher E. Vesicle pools and Ca2+ microdomains: new tools for understanding their roles in neurotransmitter release. Neuron. 1998. 20. P. 389-399.
  13. Ohno-Shosaku T., Hirata K., Sawada S., Yamamoto C. Contributions of multiple calcium channel types to GABAergic transmission in rat cultured hippocampal neurons. Neurosci. Lett. 1994. 181. P. 145-148,.
  14. Takahashi T., Forsythe I.D., Tsujimoto T. et al. Pr­esynaptic calcium current modulation by a metabotropic glutamate receptor. Science. 1996. 274. P. 594 597.
  15. Takahashi T., Momiyama A. Different types of calcium channels mediate central synaptic transmission. Na­ture. 1993. 366. P.156-158.
  16. Veselovsky N.S., Engert F., Lux H.D. Fast local superfusion technique . Pflug. Arch. 1996. 432. P. 351-354.
  17. Wilcox K.S., Dichter M.A. Paired pulse depression in cultured hippocampal neurons is due to a presynaptic mechanism independent of GABAB autoreceptor activation. J. Neurosci. 1994. 14. P. 1775-1788.
  18. Wu L.G., Borst J.G. The reduced release probability of releasable vesicles during recovery from short-term synaptic depression . Neuron. 1999. 23. P. 821-832.
  19. 19. Zucker R.S., Regehr W.G. Short-term synaptic plasticity. Annu. Rev. Physiol. 2002. 64. P. 355-405.

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