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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 10-16


T.Yu. Korol, E.P. Kostyuk, P.G. Kostyuk

    O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine


Anomalous accumulation of b-amyloid peptide in cerebral neurons plays central role in pathogenesis of Alzheimer’s dis­ease (AD). One of the essential pathogenetic factors at AD is disturbance of calcium homeostasis in neurons of central nervous system. It was determined in this work that 24-hour incubation of hippocampal cell culture with b-amyloid pep­tide caused more than twofold elevation of basal calcium concentration relatively to control value (153,4±11,5 and 71,7±5,4 nM respectively; P < 0,05, n=7). Using whole cell patch-clamp technique it was detected that calcium current density in b-amyloid-treated cells was 70 % higher (P<0.05, n=12) than in control ones. Obtained data broaden our comprehension of disturbance of molecular mechanisms of calcium homeostasis in neurons in AD, particularly mecha­nisms of elevation of basal calcium concentration by means of enhancement of calcium influx through plasmalemmal voltage-gated calcium channels.

Keywords: β-amyloid, Alzheimer’s disease, hippocampal cell culture, voltage-gated calcium channels, calcium homeostasis.


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