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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(4): 10-16


INFLUENCE OF β-AMYLOID PEPTIDE ON PLASMALEMMAL CALCIUM CHANNELS OF HIPPOCAMPAL CELL CULTURE

T.Yu. Korol, E.P. Kostyuk, P.G. Kostyuk

    O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz55.04.010

Abstract

Anomalous accumulation of b-amyloid peptide in cerebral neurons plays central role in pathogenesis of Alzheimer’s dis­ease (AD). One of the essential pathogenetic factors at AD is disturbance of calcium homeostasis in neurons of central nervous system. It was determined in this work that 24-hour incubation of hippocampal cell culture with b-amyloid pep­tide caused more than twofold elevation of basal calcium concentration relatively to control value (153,4±11,5 and 71,7±5,4 nM respectively; P < 0,05, n=7). Using whole cell patch-clamp technique it was detected that calcium current density in b-amyloid-treated cells was 70 % higher (P<0.05, n=12) than in control ones. Obtained data broaden our comprehension of disturbance of molecular mechanisms of calcium homeostasis in neurons in AD, particularly mecha­nisms of elevation of basal calcium concentration by means of enhancement of calcium influx through plasmalemmal voltage-gated calcium channels.

Keywords: β-amyloid, Alzheimer’s disease, hippocampal cell culture, voltage-gated calcium channels, calcium homeostasis.

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