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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(3): 118-124

Acid-base status and fructose diphosphatase activity in rats exposed to fluoride and induced periodontitis

J. Khalili, H.F. Biloklytska, H.V. Pogrebnyak

    Department of Therapeutic Stomatology, National Medical Academy of Postgraduate Education, named after P. L. Shupyk, Kiev, Ukraine


This study was conducted to evaluate acid-base status and fructose diphosphatase (FDPase) activity in 40 (4 groups of 10) male Wistar rats. One group of rats was left untreated as control, fed a standard diet, and given distilled water. Periodontitis model induced with 5 mg/kg NH4Cl (group 1), exposed to sodium fluoride (NaF) at the concentration 5 mg/l (group 2), exposed to NaF (5 mg/l) and supplemented with minerals and vitamins (group 3). At the termination of experimental period (30 days) the pH and pCO2 value of arterial blood were analysed. Then, the FDPase activity in the hemogenized heart, kidney, liver, mandible, pelvis, and teeth were determined by measuring inorganic phosphate that converts from fruc-tose-1.6-diphosphate and using spectrophotometer at 350 nm. The differences in the acid-base status and FDPase activity in the groups 1 and 2 were statistically significant in comparison with the control and group 3 (P<0.001). Increased FDPase activities are associated with acid-base status. The minerals and vitamins supplementation proved to restore acid-base balance, reduce toxicity and establish steady enzyme activity, which has not been previously reported.

Keywords: fluoride, ammonium chloride, pH, pCO2 , fructose diphosphatase, minerals and vitamins, rats


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