Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(3): 67-74


THE INFLUENCE OF LEY-ENKEPHALIN ON THE BILE SECRETION FUNCTION IN RAT LIVER DURING PHARMACOLOGICAL MODULATION OF THE AUTONOMOUS NERVOUS SYSTEM

L.A. Latyshenko, E.M.Reshetnik, S.P.Veselsky, M.Yu.Makarchuk

    Taras Shevchenko Kyiv National University, Ukraine


Abstract

In acute experiments on rats the influence of ley-enkephalin (1 mkg/100 g) on liver bile secretion function and changes of organic contents of bile were studied. For studying the influ­ence of opioid peptide under conditions of altered state of adreno-and cholinoreceptors, the antagonists of a- and b-adreno-receptors phentolamin (25 mkg/100 g) and obzidan (5 mkg/100 g), and the blocker of M-holinoreceptors atropine (2 mkg/100 g) were used. The pharmacological modulation of autonomous nervous systems alters the effects of ley-enkephalin on cholesecretions. Opioid peptide in the presence of antagonists of a-adrenoreceptors stimulates the processes of bile acids conjugation, in the presence of antagonists of b-adrenoreceptors it cancels the effects of ley-enkephalin. The antagonists M-holinoreceptors reverse the effects of ley-enkephalin for the opposite ones: the synthesis and transport of free bile asides and cholisterine are suppressed.

Keywords: rats, ley-enkephalin, liver bile

References

  1. Lishmanov Yu.B., Kondratiev Yu.B. Interaction of opioid and sympathetic-adrenal system in ischemic heart damage . Ros.fiziol. magazine. them. Sechenova. 1995 81. P. 77-85.
  2. Lishmanov Yu.B., Maslov LN Opioid neuro-peptides, stress and adaptive protection of the heart. Tomsk: Izd-vo Tomsk.un-ta, 1994. 352 p.
  3. Masyuk TV, Veselsky SP., Masyuk A.I. Secretory function of the liver under the action of enkephalins . Physiol. magazine. 1995. 41, N 3-4. S.3-8.
  4. Medvedev MA, Rudin IV, Garaeva AF The role of opioid receptors in the liver in the regulation of bile secretion . Bull. experiment. biology and medicine. 2006. 142, N 11. WITH. 494-496.
  5. Patent 99031324, Ukraine, MBN A61V5 . 14. The method of preparation of probioridines for determining the content of substances of lipid nature . SP. Veselsky, PS Lyashchenko, SI. Kostenko, FOR. Gorenko, L.F. Kurovskaya. N 33564A; Application 10. 05. 1999; publ. 15.02.2001; Bull. N 1.
  6. Rudin IV, Medvedev MA Opioid peptides modulate the secretion of the main determinants of bile . Bull. experiment. biology and medicine. 1997 123, N 5. WITH. 498-500.
  7. Method for determination of bile acids in biological fluids: A.s.4411066 . 14 USSR, MBP G 01 N 33. 50 . SP. Vesel'skii, P.S. Lyashchenko, I.A. Lukyanenko (USSR). N 1624322; Claimed 25.01. 1988; Publ. 30.01.1991, Bull.N 4.
  8. Bergasa N.V., Zhou J., Ravi J., Shi Q. The opioid peptide analog D-Ala2-Met-enkephalinamide decreases bile flow by a central mechanism . Peptides. 1999. 20,N 8. P. 979-986.
  9. Dhawan B.N., Cesselin F., Raghubiz R. et al. Classification of opioid receptors . Pharmacol. Rew. 1996. 48, N 4. P. 567-592.
  10. Fuder H. Opioid receptor mechanisms in the peripheral regu­lation of noradrenaline release on the heart. In: Regulatory roles of opioid peptid. New York, 1988. P. 205-217.
  11. Giaroni C, Somaini L., Marino F. et al. Modulation of enteric cholinergic neurons by hetero- and autoreceptors: cooperation among inhibitory inputs . Life Sci. 1999. N 65. P. 813-821.
  12. Holaday J.W. Cardiorespiratory effects of mu and delta opiate agonists following third or fourth ventricular in­jections . Peptides. 1982. N 3. P. 1023-1029.
  13. Ills P., Bettermann R., Ramme D. Sympathoinhibitory opioid receptors in the cardiovascular system . Brain Peptid. and Catecholamin. in Cardiovascular. Reg. 1987. N 4. R101-108.
  14. Ruth J.A., Eiden L.B. Leucine-enkephalin modulation of catecholamine positive chronotropy in rat atria is receptor-specific and calcium-dependent . Neuro­peptides. 1984. N 4. P. 101-108.
  15. Wittert G. Hope P., Pyle D. Tissue distribution of opioid receptor gene expression in the rat . Biochem. and Biophys. Res. Commun. 1996. 218, N 3. P. 877-881.
  16. Wei E.T., Lee A., Chang J.K. Cardiovascular effects of peptides related to the enkephalins and beta-caso-morphin . Life Sci. 1980. 26. P. 1517-1522.
  17. Xiao R. P., Pepe S., Spurgeon H. A. et al. Opioid peptide receptor stimulation reverses v-adrenergic effects in rat heart cells . Amer. J. Physiol. 1997. N 272. H797-H805.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2022.