THE INFLUENCE OF LEY-ENKEPHALIN ON THE BILE SECRETION FUNCTION IN RAT LIVER DURING PHARMACOLOGICAL MODULATION OF THE AUTONOMOUS NERVOUS SYSTEM
L.A. Latyshenko, E.M.Reshetnik, S.P.Veselsky, M.Yu.Makarchuk
Taras Shevchenko Kyiv National University, Ukraine
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Abstract
In acute experiments on rats the influence of ley-enkephalin (1 mkg/100 g) on liver bile secretion function and changes of organic contents of bile were studied. For studying the influence of opioid peptide under conditions of altered state of adreno-and cholinoreceptors, the antagonists of a- and b-adreno-receptors phentolamin (25 mkg/100 g) and obzidan (5 mkg/100 g), and the blocker of M-holinoreceptors atropine (2 mkg/100 g) were used. The pharmacological modulation of autonomous nervous systems alters the effects of ley-enkephalin on cholesecretions. Opioid peptide in the presence of antagonists of a-adrenoreceptors stimulates the processes of bile acids conjugation, in the presence of antagonists of b-adrenoreceptors it cancels the effects of ley-enkephalin. The antagonists M-holinoreceptors reverse the effects of ley-enkephalin for the opposite ones: the synthesis and transport of free bile asides and cholisterine are suppressed.
Keywords:
rats, ley-enkephalin, liver bile
References
- Lishmanov Yu.B., Kondratiev Yu.B. Interaction of opioid and sympathetic-adrenal system in ischemic heart damage . Ros.fiziol. magazine. them. Sechenova. 1995 81. P. 77-85.
- Lishmanov Yu.B., Maslov LN Opioid neuro-peptides, stress and adaptive protection of the heart. Tomsk: Izd-vo Tomsk.un-ta, 1994. 352 p.
- Masyuk TV, Veselsky SP., Masyuk A.I. Secretory function of the liver under the action of enkephalins . Physiol. magazine. 1995. 41, N 3-4. S.3-8.
- Medvedev MA, Rudin IV, Garaeva AF The role of opioid receptors in the liver in the regulation of bile secretion . Bull. experiment. biology and medicine. 2006. 142, N 11. WITH. 494-496.
- Patent 99031324, Ukraine, MBN A61V5 . 14. The method of preparation of probioridines for determining the content of substances of lipid nature . SP. Veselsky, PS Lyashchenko, SI. Kostenko, FOR. Gorenko, L.F. Kurovskaya. N 33564A; Application 10. 05. 1999; publ. 15.02.2001; Bull. N 1.
- Rudin IV, Medvedev MA Opioid peptides modulate the secretion of the main determinants of bile . Bull. experiment. biology and medicine. 1997 123, N 5. WITH. 498-500.
- Method for determination of bile acids in biological fluids: A.s.4411066 . 14 USSR, MBP G 01 N 33. 50 . SP. Vesel'skii, P.S. Lyashchenko, I.A. Lukyanenko (USSR). N 1624322; Claimed 25.01. 1988; Publ. 30.01.1991, Bull.N 4.
- Bergasa N.V., Zhou J., Ravi J., Shi Q. The opioid peptide analog D-Ala2-Met-enkephalinamide decreases bile flow by a central mechanism . Peptides. 1999. 20,N 8. P. 979-986.
- Dhawan B.N., Cesselin F., Raghubiz R. et al. Classification of opioid receptors . Pharmacol. Rew. 1996. 48, N 4. P. 567-592.
- Fuder H. Opioid receptor mechanisms in the peripheral regulation of noradrenaline release on the heart. In: Regulatory roles of opioid peptid. New York, 1988. P. 205-217.
- Giaroni C, Somaini L., Marino F. et al. Modulation of enteric cholinergic neurons by hetero- and autoreceptors: cooperation among inhibitory inputs . Life Sci. 1999. N 65. P. 813-821.
- Holaday J.W. Cardiorespiratory effects of mu and delta opiate agonists following third or fourth ventricular injections . Peptides. 1982. N 3. P. 1023-1029.
- Ills P., Bettermann R., Ramme D. Sympathoinhibitory opioid receptors in the cardiovascular system . Brain Peptid. and Catecholamin. in Cardiovascular. Reg. 1987. N 4. R101-108.
- Ruth J.A., Eiden L.B. Leucine-enkephalin modulation of catecholamine positive chronotropy in rat atria is receptor-specific and calcium-dependent . Neuropeptides. 1984. N 4. P. 101-108.
- Wittert G. Hope P., Pyle D. Tissue distribution of opioid receptor gene expression in the rat . Biochem. and Biophys. Res. Commun. 1996. 218, N 3. P. 877-881.
- Wei E.T., Lee A., Chang J.K. Cardiovascular effects of peptides related to the enkephalins and beta-caso-morphin . Life Sci. 1980. 26. P. 1517-1522.
- Xiao R. P., Pepe S., Spurgeon H. A. et al. Opioid peptide receptor stimulation reverses v-adrenergic effects in rat heart cells . Amer. J. Physiol. 1997. N 272. H797-H805.
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