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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(3): 37-43


SIRNA-MEDIATED SILENCING OF 5-LIPOXYGENASE GENE (ALOX5) REDUCES NECROSIS OF CARDIOMYOCYTES IN ANOXIA-REOXYGENATION

O.O. Lisovyy, V.S. Nagibin, O.V. Surova, L.V Tumanovska., V.E. Dosenko, A.A. Moibenko

    O.O. Bogomoletz Institute of Physiology NationalAcademy Sciences of Ukraine, Kyiv, Ukraine


Abstract

In experiments on the primary culture of isolated neonatal rat cardiomyocytes it was determined that cardiomyocytes express ALOX5 gene encoding enzyme 5-lipoxygenase. Anoxia-reoxygenation does not affect significantly the expression of 5-lipoxygenase mRNA in cardiomyocytes. Transfection of 5-lipoxygenase-specific small interfering RNA’s (siRNA) into cardiomyocytes lead to a significant reduction of 5-lipoxygenase mRNA expression in cardiomyocytes 24 hours after transfec-tion. ALOX5 gene silencing resulted in improved viability of cell population (by 13,3 % P<0,001) due to decreased number of necrotic (by 14,6 %, P<0,001), but not apoptotic, cells during anoxia-reoxygenation. Our results indicate that siRNA against ALOX5 effectively protects cardiomyocytes against anoxia-reoxygenation injury.

Keywords: Anoxia-reoxygenation, cardiomyocytes, 5-lipoxygenase mRNA

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