OMEGA-3 POLYUNSATURATED FATTY ACIDS ELEVATE THE EXPRESSION OF PPAR TARGET GENES
S.P. Kaplinskiy, A.M. Shysh, V.S. Nagibin, V.E. Dosenko, A.A. Moibenko
O.O.Bogomoletz Institute of Physiology, National Academy of Sciences, Kyiv, Ukraine

Abstract
In experiments on isolated neonatal cardiomyocytes and hearts of adult rats we investigated the influence of polyunsaturated fatty acids (PUFA) on expression of two peroxisome proliferator-activated receptor (PPAR) target genes - fatty acid transport protein (FATP) and interleukin-1 receptor antagonist (IL-1ra). We determined that cell membranes of adult rats heart and car-diomyocytes of neonatal rats which were born by females fed with w-3 PUFA for 4 weeks, contain more w-3 PUFA (alpha-linolenic and docosahexaenoic acids) in comparison with the control. It was shown that w-3 PUFA elevated FATP and IL-1ra mRNA expression in 2,25 (P=0,07) and 8,4 (P<0.00001) folds correspondingly in adult rats tissues. In isolated cardiomyo-cytes, the mRNA expression of FATP and IL-1ra were aug-mented 2.5 folds (P=0,22) and 8,1 folds (P<0.00001), accordingly. Our data show that w-3 PUFA stimulate PPAR target genes expression and allow to permit genetic mechanisms in cardioprotective effects of w-3 PUFA.
Keywords:
cardiomyocytes, rats, peroxisome proliferator-activated receptor,
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