Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 37-43

Omega-3 polyunsaturated fatty acids elevate the expression of ppar target genes

S.P. Kaplinskiy, A.M. Shysh, V.S. Nagibin, V.E. Dosenko, A.A. MoibenkO.

    O.O. Bogomoletz Institute of Physiology, National AcademySciences of Ukraine, Kyiv;Shemyakin-Ovchinnikov Institute of BioorganicChemistry, Russian Academy of Sciences, Russia;International Center of Molecular Physiology NationalAcademy Sciences of Ukraine, Kyiv


Abstract

In experiments on isolated neonatal cardiomyocytes and hearts of adult rats we investigated the influence of polyunsaturated fatty acids (PUFA) on expression of two peroxisome proliferator-activated receptor (PPAR) target genes - fatty acid transport protein (FATP) and interleukin-1 receptor antagonist (IL-1ra). We determined that cell membranes of adult rats heart and car-diomyocytes of neonatal rats which were born by females fed with w-3 PUFA for 4 weeks, contain more w-3 PUFA (alpha-linolenic and docosahexaenoic acids) in comparison with the control. It was shown that w-3 PUFA elevated FATP and IL-1ra mRNA expression in 2,25 (P=0,07) and 8,4 (P<0.00001) folds correspondingly in adult rats tissues. In isolated cardiomyo-cytes, the mRNA expression of FATP and IL-1ra were aug-mented 2.5 folds (P=0,22) and 8,1 folds (P<0.00001), accordingly. Our data show that w-3 PUFA stimulate PPAR target genes expression and allow to permit genetic mechanisms in cardio­protective effects of w-3 PUFA.

Full text: (PDF, in Ukrainian)

References

  1. Кейтс М. Техника липидологии. Выделение, анализ и идентификация липидов. -М.: Мир,1975. -С.322.
  2. Меерсон Ф.З., Машина СЮ. Влияние рыбьего жира с высоким содержанием полиненасыщенных жирных кислот (эйконола) на миогенный тонус и величину констрикторных и дилататорных реакций изолиро­ванной хвостовой артерии крысы // Бюл. экспер. биологии и медицины. - 1993. -№3. - С.229-231.
  3. Шиш А. М., Кукоба Т. В., Кириленко О. Є., Мойбенко О. О. Вплив препарату епадол на серцево-судинну систему при гострій ішемії–реперфузії міокарда // Вісн. Вінницьк. нац. мед. ун-ту. - 2007. -11,№2/1. -С.496-499
  4. Шиш A.M., Пивовар СМ., Кукоба Т.В., та in. Вплив модифікації жирнокислотного складу клітинних мембран на адренергічну реактивність серця та судин // Фізіол. жур. - 2004. - 50, № 6. -С.19-26.
  5. Abbate A., Salloum F.N., Vecile E. et al. Anakinra, a re­combinant human interleukin-1 receptor antagonist, in­hibits apoptosis in experimental acute myocardial infarc­tion // Circulation. - 2008. - 117, №20. - P.2670-2683.
  6. Ander B. P., Dupasquier MC, Prociuk M. A. Polyun­saturated fatty acids and their effects on cardiovascu­lar disease // Exp. Clin. Cardiol. - 2003. - 8, №4. -P.164-172.
  7. Ayalew-Pervanchon A.P., Grynberg A. Long-term effect of dietary б-linolenic acid or decosahexaenoic acid of incorporation of decosahexaenoic acid in membranes and its influence on rat heart in vivo // Amer. J. Physiol. Heart. Circulat. Physiol. - 2007. - 293. - P.H2296-H2304.
  8. Bonetti A., Marchini M., Ortolani F. Immunolocali-zation of interleukin-1 receptor antagonist in healthy and infarcted myocardium // Histol. Histopathol. -2008. - 23,№9. -P.1093-10102.
  9. 9. Breslow Jan L. n-3 Fatty acids and cardiovascular disease // Amer. J. Clin. Nutr. - 2006. - 83, №6. - P.1477-1482.
  10. 10. Calder P.C Polyunsaturated fatty acids and inflamma­tion // Biochem. Soc. Transactions. - 2005. - 33, Part 2. - P. 423-427.
  11. De Caterina R., Madonna R., Zucchi R., La Rovere M.T. Antiarrhythmic effects of omega-3 fatty acids: from epidemiology to bedside // Amer. Heart J. - 2003. -146,№3. - P.420-430.
  12. Dinarello C.A. Interleukin 1 and interleukin 18 as me­diators of inflammation and the aging process // Amer. J. Clin. Nutrit. - 2006. - 83. - P.447S-455S.
  13. Grimble R.F., Howell W.M., O’Reilly G. et al. The ability of fish oil to suppress tumor necrosis factor a production by peripheral blood mononuclear cells in healthy men is associated with polymorphisms in genes that influence tumor necrosis factor a production // Ibid. - 2002. - 76. - P.454-459.
  14. Heikkinen S., Auwerx J., Argmann C.A. PPARr in hu­man and mouse physiology // Bioch. Biophys. Acta. -2007. - 1771. - P.999-1013.
  15. Hirafuji M., Machida T., Hamaue N., Minami M. Car­diovascular protective effects of n-3 polyunsaturated fatty acids with special emphasis on docosahexaenoic acid // J. Pharmacol Sci. - 2003. - 92, №4. - P.308-316.
  16. Holm T., Berge R.K., Anderassen A.K. et al. Omega-3 fatty acids enhance tumor necrosis factor-alpha levels in heart transplant recipients // Transplantation. - 2001. -72. - P.706-711.
  17. Holub B.J. Clinical nutrition: 4. Omega-3 fatty acids in cardiovascular care // Canad. Med. Associat. J. -2002. - 166,№5. - P.608-615.
  18. Hooper L., Thompson R. L., Harrison R. A. et al. Risks and benefits of omega 3 fats for mortality, cardiovascu­lar disease, and cancer: systematic review // British. Med. J. - 2006. - 332. - P.752-760.
  19. 19. Knouff C, Auwerx J. Peroxisome proliferator-activated receptor-gamma calls for activation in moderation: les­sons from genetics and pharmacology // Endocrinol. Rev. - 2004. - 25. - P.899-918.
  20. 20. Kuroda T., Hirota H., Fujio Y. Carbacyclin induces carnitine palmitoyltransferase-1 in cardiomyocytes via peroxisome proliferator-activated receptor (PPAR) delta independent of the IP receptor signaling path­way // J. Mol. Cell. Cardiol. - 2007. - 43. - P.54-62.
  21. Leaf A., Kang X., Xiao Y., Billman G. Clinical Preven­tion of Sudden Cardiac Death by n-3 Polyunsaturated Fatty Acids and Mechanism of Prevention of Arrhyth-mias by n-3 Fish Oils // Circulation. - 2003. - 107. -P.264-266.
  22. Lehrke M., Lazar M.A. The many faces of PPARgam-ma // Cell. - 2005. - 123. - P.993-999.
  23. Owen A.J., Peter-Przyborowska B.A., Hoy A.J., McLennan P.L. Dietary fish oil dose- and time-response effects on cardiac phospholipid fatty acid composition // Lipids. - 2004. - 39, № 10. - P.955-961.
  24. Pound E.M., Kang J.X., Leaf A. Partitioning of poly­unsaturated fatty acids, which prevent cardiac arrhythmias, into phospholipid cell membranes // J. Lipid Res. - 2001. - 42. -P.346-351.
  25. Power G.W., Newsholme E.A. Dietary fatty acids influ­ence the activity and metabolic control of mitochondrial carnitine palmitoyltransferase I in rat heart and skeletal muscle // J. Nutr. - 1997. - 127, № 11. - P.2142-2150.
  26. Rosen E.D., Spiegelman B.M. PPARgamma: a nuclear regulator of metabolism, differentiation, and cell growth // J. Biol. Chem. - 2001. - 276. - P.37731-37734.
  27. Scheller J., Ohnesorge N., Rose-John S. Interleukin-6 trans-signaling in chronic inflammation and cancer // Scand. J. Immunol. - 2006. - 63. - P321-329.
  28. Stienstra R., Mandard S., Tan N.S. et al. The Interleukin-1 receptor antagonist is a direct target gene of PPARa in liver // J. Hepatol. - 2007. - 46. - P.869-877.
  29. 29. Sun H., Berquin I.M., Edwards I.J. Omega-3 polyun­saturated fatty acids regulate syndecan-1 expression in human breast cancer cells // Cancer Res. -2005. - 65, №10. - P.4442-4447.
  30. 30. Sun H., Berquin I.M., Owens R.T. et al. Peroxisome proliferator-activated receptor gamma-mediated up-regulation of syndecan-1 by n-3 fatty acids promotes apoptosis of human breast cancer cells // Ibid. - 2008. -688. - P.2912-2919.
  31. von Schacky C. n-3 PUFA in CVD: influence of cytokine polymorphism // Proc. Nutrit. Soc. - 2007. - 66. – P.166-170.
  32. Zhang M., Chen L. Status of cytokines in ischemia reperfusion induced heart injury // Cardiovasc. Hematol. Disord. Drug. Targets. - 2008. - 8, №3. -P.161-172.
  33. Zhou X.R., Sun C.H., Liu J.R., Zhao D. Dietary conjugated linoleic acid increases PPARg gene expres­sion in adipose tissue of obese rat, and improves insulin resistance // Grow. Horm. Res. - 2008. - 18, №5. -P.361-368.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.