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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 30-36

Radiation does not affect calcium-dependent pathway ofnitroglycerine-evoked relaxation of rat aorta.

S.M. Zelenskyi,A.I. SolovyoV.

    Institute of Pharmacology and Toxycology AcademyMedical Sciences of Ukraine, Kyiv


Abstract

The effects of nitroglycerine (NG, 10-7 -10-4 M) on contractile force and intracellular calcium concentration ([Ca2+]i) in deendotelized thoracic aorta smooth muscles (SM) precontracted with high K+ in control and g-irradiated rats (6Gy) were investigated. NG-induced activation of soluble guanylate cyclase (sGC) as dominating pathway of NG- induced SM relaxation without related decrease in [Ca2+]i level is demonstrated. Radiation was without effect on this sGC-dependent and [Ca2+]i - independent NG-induced relaxation of vascular smooth muscle but decreased the basal levels of [Ca2+]i by (28,4±11,9 )% (6Gy, 9th-day).

Full text: (PDF, in Ukrainian)

References

  1. Blatter L.A., Wier W.G. Nitric oxide decreases [Ca2+] in vascular smooth muscle by inhibition of the calciu m current // Cell Calcium. - 1994. - 15, Feb. - P. 122-131.
  2. Clapp L., Garney A. Modulation of calcium movements by nitroprusside in isolated smooth muscle cells // Pflug. Arch. Eur. J. Physiol. - 1991. - 418, № 5. - P. 462–470.
  3. Collins P., Griffith T.M., Henderson A.H., Lewis M.J. Endothelium-derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate-mediated effect // J. Physiol. - 1986. - 381, Dec. - P. 427-437.
  4. Furukawa K.-I., Ohshima N., Yawada-Iwata Y., Shigekawa M. Cyclic cGMP stimulates Na+/Ca2+ ex­change in vascular smooth muscle cells in primary culture // J. Biol. Chem. - 1991. - 266, № 19. -P. 12377-123341.
  5. Gregory R.J. Thatcher, Adrian C. Nicolescu, Brian M. Bennett, Violeta Toader. Nitrates and NO release: contemporary aspects in biological and medicinal chemistry //J. Free Radical. Biol. and Med. - 2004. -37, №8. - P. 1122-1143.
  6. Griffith T.M., Edwards D.H., Lewis M.J., Henderson A.H. Evidence that cyclic guanosine monophosphate (cGMP) mediates endothelium-dependent relaxation // Eur. J. Pharmacol. - 1985. - 112, № 2. - P. 195-202.
  7. Grynkiewicz G., Poenie G.M., Tsien R.Y. A new generation of Ca2+ indicators with greatly improved fluores­cence properties // J. Biol. Chem. - 1985. - 260, March. -P. 3440-3450.
  8. Himpens B., Matthijs G., Somlyo A.V. et al. Cytoplasmic free calcium, myosin light chain phosphorylation, and force in phasic and tonic smooth muscle// J. Gen. Physiol. - 1988. - 92, Dec. - P. 713-729.
  9. 9. Kleschyov A.L., Oelze M., Daiber A. et al. Does Nitricoxide mediate the vasodilator activity of nitroglycerin?// Circulat. Res. - 2003. - 93. - P.104-112.
  10. 10. Lehen’kyi V.V., Zelensky S.N., Stefanov A.V., Soloviev A.I. Effects of nitric oxide donors on vascular smooth muscles depend on a type of vascular smooth-muscle preactivation // Cardiovascular. Toxicol. - 2002. - 2, №2. - P. 151-160.
  11. Lincoln T., Komalavilas P., Cornwell T. Pleiotropic regulation of vascular smooth muscle tone by cyclic GMP-dependent protein kinase // Hypertension. - 1994. -23. - P. 1141-1147.
  12. Menendez J.C, Casanova D, Amado J.A. et al. Effects of radiation on endothelial function // Intern. J.Rad. Oncol, Biol, Phys. - 1998. - 41, № 4. - P. 905-913.
  13. Schrammel A., Behrends S., Schmidt K. et al. Characterization of 1H-[1,2,4]oxadiazolo[4,3-a]quino-xalin-1-one as a heme-site inhibitor of nitric oxide-sen­sitive guanylyl cyclase // Mol. Pharmacol. - 1996. -50,№1. - P.1-5.
  14. Soloviev A., Lehen’kyi V., Zelensky S., Hellstrand P. Nitric oxide relaxes rat tail artery smooth muscle by cyclic GMP-independent decrease in calcium sensitivity of myofilaments // Cell Calcium. - 2004. - 36, № 2. -P. 165-173.
  15. Soloviev A., Stefanov A., Tishkin S. et al. Saline containing phosphatidylcholine liposomes possess the ability to restore endothelial function damaged resulting from r-irradiation // J. Physiol. and Pharmacol. - 2002. -53. – P. 707-712.
  16. Soloviev A., Tishkin S., Parshikov A. et al. Mechanisms of endothelium dysfunction after ionized radiation: se­lective impairment of the nitric oxide component of endo-thelium-dependent vasodilation // Br. J. Pharmacol. -2003. - 138. - 5. - P.837-844.
  17. Soloviev A., Tishkin S., Zelensky S. et al. Ionizing radiation alters myofilament calcium sensitivity in vas­cular smooth muscle: potential role of protein kinase // Amer. J. Physiol. - 2005. - 289. - P.755-762.
  18. Somlyo A.P., Somlyo A.V. Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II:Modulated by G Pro­teins, Kinases, and Myosin Phosphatase // Physiol. Rev. -2003. - 83. - P.1325-1358.
  19. 19. Tran Nguyen N. P., Spitzbarth E., Robert A. et al. Ni­tric oxide lowers the calcium sensitivity of tension in the rat tail artery// J. Physiol. - 1998. - 507, № 1. -P. 163-174.
  20. 20. Zhong G.Z., Chen F.R., Bu D.F. et al. Cobalt-60 gamma radiation increased the nitric oxide generation in cultured rat vascular smooth muscle cells// Life Sci. - 2004. -74,№25. - P.3055-3063.
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