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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 30-36

RADIATION DOES NOT AFFECT CALCIUM-DEPENDENT PATHWAY OF NITROGLYCERINE-EVOKED RELAXATION OF RAT AORTA

S.M. Zelenskyi, A.I. Solovyov

    Institute of Pharmacology and Toxycology AcademyMedical Sciences of Ukraine, Kyiv, Ukraine


Abstract

The effects of nitroglycerine (NG, 10-7 -10-4 M) on contractile force and intracellular calcium concentration ([Ca2+]i) in deendotelized thoracic aorta smooth muscles (SM) precontracted with high K+ in control and g-irradiated rats (6Gy) were investigated. NG-induced activation of soluble guanylate cyclase (sGC) as dominating pathway of NG- induced SM relaxation without related decrease in [Ca2+]i level is demonstrated. Radiation was without effect on this sGC-dependent and [Ca2+]i - independent NG-induced relaxation of vascular smooth muscle but decreased the basal levels of [Ca2+]i by (28,4±11,9 )% (6Gy, 9th-day).

Full text: (PDF, in Ukrainian)

References

  1. Blatter L.A., Wier W.G. Nitric oxide decreases [Ca2+] in vascular smooth muscle by inhibition of the calciu m current . Cell Calcium. 1994. 15, Feb. P. 122-131.
  2. Clapp L., Garney A. Modulation of calcium movements by nitroprusside in isolated smooth muscle cells . Pflug. Arch. Eur. J. Physiol. 1991. 418, N 5. P. 462-470.
  3. Collins P., Griffith T.M., Henderson A.H., Lewis M.J. Endothelium-derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate-mediated effect . J. Physiol. 1986. 381, Dec. P. 427-437.
  4. Furukawa K. I., Ohshima N., Yawada-Iwata Y., Shigekawa M. Cyclic cGMP stimulates Na+. Ca2+ ex­change in vascular smooth muscle cells in primary culture . J. Biol. Chem. 1991. 266, N 19. P. 12377-123341.
  5. Gregory R.J. Thatcher, Adrian C. Nicolescu, Brian M. Bennett, Violeta Toader. Nitrates and NO release: contemporary aspects in biological and medicinal chemistry .J. Free Radical. Biol. and Med. 2004. 37, N 8. P. 1122-1143.
  6. Griffith T.M., Edwards D.H., Lewis M.J., Henderson A.H. Evidence that cyclic guanosine monophosphate (cGMP) mediates endothelium-dependent relaxation . Eur. J. Pharmacol. 1985. 112, N 2. P. 195-202.
  7. Grynkiewicz G., Poenie G.M., Tsien R.Y. A new generation of Ca2+ indicators with greatly improved fluores­cence properties . J. Biol. Chem. 1985. 260, March. P. 3440-3450.
  8. Himpens B., Matthijs G., Somlyo A.V. et al. Cytoplasmic free calcium, myosin light chain phosphorylation, and force in phasic and tonic smooth muscle. J. Gen. Physiol. 1988. 92, Dec. P. 713-729.
  9. Kleschyov A.L., Oelze M., Daiber A. et al. Does Nitricoxide mediate the vasodilator activity of nitroglycerin?. Circulat. Res. 2003. 93. P.104-112.
  10. Lehen’kyi V.V., Zelensky S.N., Stefanov A.V., Soloviev A.I. Effects of nitric oxide donors on vascular smooth muscles depend on a type of vascular smooth-muscle preactivation . Cardiovascular. Toxicol. 2002. 2, N 2. P. 151-160.
  11. Lincoln T., Komalavilas P., Cornwell T. Pleiotropic regulation of vascular smooth muscle tone by cyclic GMP-dependent protein kinase . Hypertension. 1994. 23. P. 1141-1147.
  12. Menendez J.C, Casanova D, Amado J.A. et al. Effects of radiation on endothelial function . Intern. J.Rad. Oncol, Biol, Phys. 1998. 41, N 4. P. 905-913.
  13. Schrammel A., Behrends S., Schmidt K. et al. Characterization of 1H-[1,2,4]oxadiazolo[4,3-a]quino-xalin-1-one as a heme-site inhibitor of nitric oxide-sen­sitive guanylyl cyclase . Mol. Pharmacol. 1996. 50,N 1. P.1-5.
  14. Soloviev A., Lehen’kyi V., Zelensky S., Hellstrand P. Nitric oxide relaxes rat tail artery smooth muscle by cyclic GMP-independent decrease in calcium sensitivity of myofilaments . Cell Calcium. 2004. 36, N 2. P. 165-173.
  15. Soloviev A., Stefanov A., Tishkin S. et al. Saline containing phosphatidylcholine liposomes possess the ability to restore endothelial function damaged resulting from r-irradiation . J. Physiol. and Pharmacol. 2002. 53. P. 707-712.
  16. Soloviev A., Tishkin S., Parshikov A. et al. Mechanisms of endothelium dysfunction after ionized radiation: se­lective impairment of the nitric oxide component of endo-thelium-dependent vasodilation . Br. J. Pharmacol. 2003. 138. 5. P.837-844.
  17. Soloviev A., Tishkin S., Zelensky S. et al. Ionizing radiation alters myofilament calcium sensitivity in vas­cular smooth muscle: potential role of protein kinase . Amer. J. Physiol. 2005. 289. P.755-762.
  18. Somlyo A.P., Somlyo A.V. Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II:Modulated by G Pro­teins, Kinases, and Myosin Phosphatase . Physiol. Rev. 2003. 83. P.1325-1358.
  19. Tran Nguyen N. P., Spitzbarth E., Robert A. et al. Ni­tric oxide lowers the calcium sensitivity of tension in the rat tail artery. J. Physiol. 1998. 507, N 1. P. 163-174.
  20. Zhong G.Z., Chen F.R., Bu D.F. et al. Cobalt-60 gamma radiation increased the nitric oxide generation in cultured rat vascular smooth muscle cells. Life Sci. 2004. 74,N 25. P.3055-3063.
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