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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 17-23


O.I. Bondarenko, V.F. Sagach

    O.O. Bogomoletz Institute of Physiology, National AcademySciences of Ukraine, Kyiv, Ukraine


Because the sustained component of hyperpolarization of en­dothelial cells evoked by acetylcholine in isolated rat aorta may partially be mediated by the reversed Na+-Ca2+ exchanger and Na+-K+ATPase, the mechanisms which transport Na+ out of cells, we compared the electrical responses of endothelial cells from isolated rat aorta to acetylcholine with other Ca2+ mobilizing agents and studied the effect of nicotine on endothelial membrane potential in order to asses the functional activity of nicotinic cholinoreceptors. Ca2+ ionophores A23187 and ionomycin, as well as inhibitors of endoplasmic reticulum Ca2+ ATPase cyclopiazonic acid and 2,5-di-tert-butylhydroquinone evoked a short-lived hyperpolarization which turned to a sustained depolar­ization of endothelial cells, a time course that substantially dif­fered from that evoked by acetylcholine. Nicotine evoked a Na+ dependent depolarization of endothelial cells confirming functional activity of nicotinic cholinoreceptors in rat aortic endothelial cells. The results suggest that stimulation of Na+ permeant nicotinic receptors by acetylcholine may contribute to sustained hyperpolarizatiom via stimulation of Na+ extrusion mechanisms.

Keywords: en­dothelial cells, Na+-Ca2+ exchanger, Na+-K+ATPase


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