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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 17-23


O.I. Bondarenko, V.F. Sagach

    O.O. Bogomoletz Institute of Physiology, National AcademySciences of Ukraine, Kyiv, Ukraine


Because the sustained component of hyperpolarization of en­dothelial cells evoked by acetylcholine in isolated rat aorta may partially be mediated by the reversed Na+-Ca2+ exchanger and Na+-K+ATPase, the mechanisms which transport Na+ out of cells, we compared the electrical responses of endothelial cells from isolated rat aorta to acetylcholine with other Ca2+ mobilizing agents and studied the effect of nicotine on endothelial membrane potential in order to asses the functional activity of nicotinic cholinoreceptors. Ca2+ ionophores A23187 and ionomycin, as well as inhibitors of endoplasmic reticulum Ca2+ ATPase cyclopiazonic acid and 2,5-di-tert-butylhydroquinone evoked a short-lived hyperpolarization which turned to a sustained depolar­ization of endothelial cells, a time course that substantially dif­fered from that evoked by acetylcholine. Nicotine evoked a Na+ dependent depolarization of endothelial cells confirming functional activity of nicotinic cholinoreceptors in rat aortic endothelial cells. The results suggest that stimulation of Na+ permeant nicotinic receptors by acetylcholine may contribute to sustained hyperpolarizatiom via stimulation of Na+ extrusion mechanisms.

Keywords: en­dothelial cells, Na+-Ca2+ exchanger, Na+-K+ATPase


    Bondarenko OI, Sagach VF Participation of mitochondria in hyperpolarization of endothelial cells under the action of acetylcholine . Fiziol Zh. 2006. 52, N 5. P.6-11. Argacha J. F., Fontaine D., Adamopoulos D. et al. Acute effect of sidestream cigarette smoke extract on vascu­lar endothelial function . J.Cardiovasc.Pharmacol. 2008. 52. N 3. P.262-267. Blaustein M.P., Lederer W.J. Sodium. calcium exchange: its physiological implications . Physiol. Rev. 1999. 79. P.763-854. Bondarenko A. Sodium-calcium exchanger contributes to membrane hyperpolarization of intact endothelial cells from rat aorta during acetylcholine stimulation . Brit. J. Pharmacol. 2004. 143. P.9-18. Bondarenko A., Sagach V. Na+, K+-ATPase is involved in the sustained Ach-induced hyperpolarization of en­dothelial cells from rat aorta . Ibid. 2006. 149. P.958-965. Bruggmann D., Lips K. S., Pfeil U. et al. Rat arteries contain multiple nicotinic acetylcholine receptor alpha-subunits . Life Sci. 2003. 72. P.2095-2099. Conklin B. S., Surowiec S. M., Ren Z. et al. Effects of nicotine and cotinine on porcine arterial endothelial cell function . J.Surg.Res. 2001. 95. N 1. P.23-31. Cooke J. P. Angiogenesis and the role of the endothe­lial nicotinic acetylcholine receptor . Life Sci. 2007. 80. P.2347-2351. Chadha P.S., Moffatt J.D., Lever R. The effect of a se­lective a7-nicotinic acetylcholine receptor antagonist on en-dothelium-dependent relaxation in rat mesenteric arteries . Proc. Austral. Physiolog. Soc. 2007. 38. P.88. Itoh T., Kanmura Y., Kuriyama H. A23187 increases calcium permeability of store sites more then of sur­face membranes in the rabbit mesenteric artery . J. Physiol. 1985. 359. P.467-484. Kawashima K., Watanabe N., Oohata H. et al. Synthe­sis and release of acetylcholine by cultured bovine arterial endothelial cells . Neurosci Lett. 1990. 119. P.156-158. Macklin K.D., Maus A.D.J., Pereira E.F.R. et al. Hu­man vascular endothelial cells express functional nicotinic acetylcholine receptors . J. Pharmacol. Exp. Therap. 1998. 287. P.435-439. Malli R., Frieden M., Osibow K. et al. Sustained Ca2+ transfer across mitochondria is essential for mitochon­drial Ca2+ buffering, store-operated Ca2+ entry, and Ca2+ store refilling . J. Biol. Chem. 2003. 278. N 45. P.769-779. Moccia F., Frost C, Berra-Romani R. et al. Expres­sion and function of neuronal nicotinic ACh receptors in rat microvascular endothelial cells . Amer. J.Physiol. Heart Circulat. Physiol. 2004. 286. N 2. H486-H491. Suzuki N., Ishii Y., Kitamura S. Effects of nicotine on production of endothelin and eicosanoid by bovine pul­monary artery endothelial cells . Prostagland. Leukot. Essent. Fatty Acids. 1994. 50. P.193-197. Tracey W. R . Peach M. J. Differential muscarinic receptor mRNA expression by freshly isolated and cultured bovine aortic endothelial cells . Circulat. Res. 1992. 70 (2). P. 234-240. Tonnessen B.H., Severson S.R., Hurt R.D., Miller V.M. Modulation of nitric-oxide synthase by nicotine . J. Pharmacol. Exp. Therap. 2000. 295. P.601-606. Tsuneki H., Ito K., Sekizaki N. et al. Nicotinic enhancement of proliferation in bovine and porcine ce­rebral microvascular endothelial cells . Biol. Pharm. Bull. 2004 27. N 12. P.1951-1956. Usachev Y.M., Marchenko S.M., Sage S.O. Cytosolic calcium concentration in resting and stimulated endot­helium of excised intact rat aorta . J. Physiol. 1995. 489. P.309-317. Wang Y, Pereira E.F., Maus A.D. et al. Human bron­chial epithelial and endothelial cells express alpha 7 nicotinic acetylcholine receptor . Mol. Pharmacol. 2001. 60. P.1201-1209. Ziche M., Morbidelli L., Masini E. et al. Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P . J. Clin. Invest. 1994. 94, N 5. P. 2036-2044.

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