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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 17-23


Stimulation of nicotinic cholinoreceptors modulates theelectrical responses of endothelial cells to acetylcholine

O.I. Bondarenko, V.F. SagacH.

    O.O. Bogomoletz Institute of Physiology, National AcademySciences of Ukraine, Kyiv


Abstract

Because the sustained component of hyperpolarization of en­dothelial cells evoked by acetylcholine in isolated rat aorta may partially be mediated by the reversed Na+-Ca2+ exchanger and Na+-K+ATPase, the mechanisms which transport Na+ out of cells, we compared the electrical responses of endothelial cells from isolated rat aorta to acetylcholine with other Ca2+ mobilizing agents and studied the effect of nicotine on endothelial membrane potential in order to asses the functional activity of nicotinic cholinoreceptors. Ca2+ ionophores A23187 and ionomycin, as well as inhibitors of endoplasmic reticulum Ca2+ ATPase cyclopiazonic acid and 2,5-di-tert-butylhydroquinone evoked a short-lived hyperpolarization which turned to a sustained depolar­ization of endothelial cells, a time course that substantially dif­fered from that evoked by acetylcholine. Nicotine evoked a Na+ dependent depolarization of endothelial cells confirming functional activity of nicotinic cholinoreceptors in rat aortic endothelial cells. The results suggest that stimulation of Na+ permeant nicotinic receptors by acetylcholine may contribute to sustained hyperpolarizatiom via stimulation of Na+ extrusion mechanisms.

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