Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(2): 3-10


S.V. Goncharov1, V.E. Dosenko1, N.V. Haitovich2, A.A.Moibenko1

  1. O.O. Bogomoletz Institute of Physiology, National Academy Sciences of Ukraine, Kyiv, Ukraine
  2. O.O. Bogomoletz National Medical University, Kyiv, Ukraine


Large multifunctional protease LMP2 (Arg60 -> His), LMP7 (Lys145 -> Gln) and PSMA6 (C–8 -> G) gene allelic polymor­phisms in 147 young patients with essential hypertension andin 208 practically healthy people were determinated. It was shown that interrelation of genotypes Arg/Arg, Arg/His and His/His in LMP2 gene polymorphisms account 42,5 %, 46,4% and 11,1% correspondingly (in control - 63,9%, 28,6%, 7,5%; P=0.001 by c2-test). Allelic variants of PSMA6 dispense the next manner: C/C - 76,2%, C/G - 21,1%, G/G - 2,7% in adoles-cents with EH (in control - 69,8%, 29,7% and 0,5% correspondingly, P=0,047). Analysis of LMP7 gene poly­morphism showed identical frequency of different genotypes in patients (Lys/Lys - 92,4%, Lys/Gln - 7,6%, Gln/Gln - 0%) and practically healthy people (97,3%, 2,7%, 0% correspon­dingly; P=0.16). Obtained data suggest the LMP2 and PSMA6 gene polymorphisms significance as the risk factors of essen­tial hypertension in adolescents.

Keywords: protease LMP2


    Goldberg A., Eledge C, Harper J.W. Mechanisms of cell death . World of Science. 2001, N 2. p. 32-37. Dosenko VE, Zagoriy VY, Moybenko AA Influence of proteasomal proteolysis on NO-synthase activity in isolated platelets . Ukr. biochemistry. magazine. 2005. 77. P.39-42. Dosenko VE, Mikhalchuk DV, Zagoriy VY et al. Frequency of allelic polymorphism of genes encoding catalytic subunits of the immunoproteasome in patients with acute coronary syndrome . Cytology and Genetics. 2005. 39, N 6. P.50-54. Dosenko VE, Mikhalchuk DV, Zagory VY etc. Functional significance of allelic polymorphism of genes encoding catalytic subunits of the immunoproteasome . Fiziol Zh. 2005. 51, N 6. P.3-10. Maidannik VG, Khaitovich MV, Missouri LI etc. Violation of the daily profile of blood pressure in children with autonomic dysfunction . Pediatrics, obstetrics and gynecology. 2003. N 6. P.23-28. Bokkala S., Joseph S.K. Angiotensin II-induced down-regulation of inositol trisphosphate receptors in WB rat liver epithelial cells. Evidence for involvement of the proteasome pathway . J. Biol. Chem. 1997. 272, N 19. P. 12454-12461. Cunha F.M., Berti D.A., Ferreira Z.S. et al. Intracellu­lar peptides as natural regulators of cell signaling . Ibid. 2008. 283, N 36. P.24448-24459. Deng G.Y., Muir A., Maclaren N.K., She J.X. Associa­tion of LMP2 and LMP7 genes within the major histo­compatibility complex with insulin-dependent diabetes mellitus: population and family studies . Amer. J. Hum. Genet. 1995. 56. P. 528-534. Egerer K., Kuckelkorn U., Rudolph P.E. et al. Circulat­ing proteasomes are markers of cell damage and immunologic activity in autoimmune diseases . J. Rheumatol. 2002. 29, N 10. P. 2045-2052. Gaczynska M., Goldberg A.L., Tanaka K. et al. Proteasome subunits X and Y alter peptidase activi­ties in opposite ways to the interferon-gamma-induced subunits LMP2 and LMP7 . J. Biol. Chem. 1996. 271,N 29. P.17275-17280. Goldberg A.L., Cascio P., Saric T., Rock K.L. The importance of the proteasome and subsequent pro­teolytic steps in the generation of antigenic peptides . Mol. Immunol. 2002. 39, N 3 4. P. 147-164. Goldberg AL. Protein degradation and protection against misfolded or damaged proteins . Nature. 2003. 426. P.895-899. Kawaguchi Y., Ikegami H., Fukuda M. et al. Absence of association of TAP and LMP genes with type 1 (insulin-dependent) diabetes mellitus . Life Sci. 1994. 54,N 26. P. 2049-2053. Kuzushita N., Sugimoto Y, Sasaki Y, Hayashi N. Involvement of TAP2 and LMP7 gene polymorphisms in HCV infection . Nippon Rinsho. 2001. 59. P.1248-1253. Li H., Armando I., Yu P. et al. Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells . J. Clin. Invest. 2008. 118, N 6. P.2180-2189. Li N., Chen L., Yi F. et al. Salt-sensitive hypertension induced by decoy of transcription factor hypoxia-induc­ible factor-1alpha in the renal medulla . Circulat. Res. 2008. 102,N 9. P.1101-1108. Maksymowych W.P., Russell A.S. Polymorphism in the LMP2 gene influences the relative risk for acute ante­rior uveitis in unselected patients with ankylosing spondylitis . Clin. Invest. Med. 1995. 18. P.42-46. Maksymowych W.P., Wessler A., Schmitt-Egenolf M. Et al. Polymorphism in an HLA linked proteasome gene influences phenotypic expression of disease in HLA-B27 positive individuals . J. Rheumatol. 1994. 21, N 4. P.665-669. Marfella R., Di Filippo C, Portoghese M. et al. Proteasome activity as a target of hormone replacement therapy-dependent plaque stabilization in postmeno­pausal women . Hypertension. 2008. 51, N 4. P.1135-1141. Nagata N., Oshida T., Yoshida N.L. et al. Analysis of highly expressed genes in monocytes from atopic dermatitis patients . Int. Arch. Allergy Immunol. 2003. 132,N 2. P.156-167. Okamoto H., Takaoka M., Ohkita M. et al. A proteasome inhibitor lessens the increased aortic endothelin-1 content in deoxycorticosterone acetate-salt hypertensive rats . Eur. J. Pharmacol. 1998. 350, N 1. P.11-12. Ozaki K, Sato H, Iida A. A functional SNP in PSMA6 confers risk of myocardial infarction in the Japanese population . Nat. Genet. 2006. 38, N 8. P.921-925. Page E.L., Chan D.A., Giaccia A.J. et al. Hypoxia-inducible factor-1{alpha} stabilization in nonhypoxic conditions: role of oxidation and intracellular ascorbatedepletion . Mol. Biol. Cell. 2008. 19,N 1. P.86-94. Pryhuber K.G., Murray K.J., Donnelly P. et al. Poly­morphism in the LMP2 gene influences disease sus­ceptibility and severity in HLA-B27 associated juve­nile rheumatoid arthritis . J. Rheumatol. 1996. N 23. P.747-752. Stangl V., Lorenz M., Meiners S. et al. Long-term up-regulation of eNOS and improvement of endothelial function by inhibition of the ubiquitin-proteasome pathway . FASEB J. 2004. 18, N 2. P.272-279. Takaoka M., Ohkita M., Itoh M. et al. A proteasome inhibitor prevents vascular hypertrophy in deoxycor­ticosterone acetate-salt hypertensive rats . Clin. Exp. Pharmacol. Physiol. 2001. 5, N 6. P.466-468. Takaoka M., Okamoto H., Ito M. et al. Antihyperten­sive effect of a proteasome inhibitor in DOCA salt hypertensive rats . Life Sci. 1998. 63, N 4. P.65-70. Takashima N., Shioji K., Kokubo Y. et al. Validation of the association between the gene encoding proteasome sub-unit alpha type 6 and myocardial infarction in a Japanese population . Circulat. J. 2007. 71, N 4. P.495-498. The fourth report on the diagnosis, evaluation, and treatment of high blood pressure in children and ado­lescents . Pediatrics. 2004. 114, N 2. P.555-574. van Endert P.M., Liblau R.S., Patel S.D. et al. Major histocompatibility complex-encoded antigen process­ing gene polymorphism in IDDM . Diabetes. 1994. 43, N 1. P.110-117. Vinasco J., Fraile A., Nieto A. et al. Analysis of LMP and TAP polymorphisms by polymerase chain reaction-restric­tion fragment length polymorphism in rheumatoid arthritis . . Ann. Rheum. Dis. 1998. 57. P.33-37. Zhang W., Meng H., Li Z.H. et al. Regulation of STIM1, store-operated Ca2+ influx, and nitric oxide generation by retinoic acid in rat mesangial cells . Amer. J. Physiol. Renal. Physiol. 2007. 292, N 3. F1054-1064. Zoeger A., Blau M., Egerer K. et al. Circulating proteasomes are functional and have a subtype pat­tern distinct from 20S proteasomes in major blood cells . Clin. Chem. 2006. 52, N 11. P.2079-2086.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.